Cell No. : Cell Name
RCB2311 : JFP-K2
update : 2025/01/16
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| Comment | Hybridoma producing monoclonal antibody against GFP. Rat IgG2a monoclonal antibody. |
| Comment from the depositor | |
| Terms and conditions | 1) Material is considered the proprietary property of Mitsubishi Kagaku Institute of Life Science (MITILA) and Mitsubishi Chemical Corporation (MCC) . 2) Recipient Investigator agrees not to use Material for profit making or commercial purposes. 3) Recipient Investigator shall acknowledge the source of Material in any publication containing experimental results obtained with the use of Material. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Neurochem 1999;19:3918-3925) designated by the DEPOSITOR is required. 5) At the conclusion of the research for which Material was requested, Recipient shall dispose of unused Material in an appropriate legal manner. 6) After providing Material, RIKEN BRC shall inform MCC of followings;names of Recipient and Recipient Investigator, purpose and date. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Takayama, Hajime
|
| Originator |
Fujita, Shinobu
|
| Year of deposit |
2006
|
| Another name |
858C10
|
| Animal |
_mouse x rat
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_C480
|
|
hybridoma
|
Fusion_cell_1 name |
P3-X63-Ag8.653
|
| Fusion_cell_1 strain |
mouse
|
| Fusion_cell_2 name |
spleen cell
|
| Fusion_cell_2 strain |
rat, Wistar
|
|
Fusion method |
PEG
|
|
Antibody name |
JFP-K2
|
| Subclass |
IgG2a
|
| Immunogen |
recombinant GFP
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|
Antigen_name |
GFP
|
| Antigen_m.w. |
30kDa
|
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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DMEM (low glucose) + 10% FBS
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| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
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Culture information
|
Passage ratio |
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1 : 4 split
|
| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Antibody subclass |
|
IgG2a
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
5
|
| User's Publication |
0
|
| Reference |
|
2150
Hennou S, Kato A, Schneider EM, Lundstrom K, Gähwiler BH, Inokuchi K, Gerber U, Ehrengruber MU. H
Homer-1a/Vesl-1S enhances hippocampal synaptic transmission
Eur J Neurosci
2003
18(4):811-9.
PubMed ID: 12925007
DOI: 10.1046/j.1460-9568.2003.02812.x
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2167
Hasuwa H, Kaseda K, Einarsdottir T, Okabe M.
Small interfering RNA and gene silencing in transgenic mice and rats
FEBS Lett
2002
532(1-2):227-30
PubMed ID: 12459495
DOI: 10.1016/s0014-5793(02)03680-3
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2218
Tamaki T, Akatsuka A, Ando K, Nakamura Y, Matsuzawa H, Hotta T, Roy RR, Edgerton VR.
Identification of myogenic-endothelial progenitor cells in the interstitial spaces of skeletal muscle
J Cell Biol
2002
157(4):571-7.
PubMed ID: 11994315
DOI: 10.1083/jcb.200112106
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3615
Hayashi K, Shirao T.
Change in the shape of dendritic spines caused by overexpression of drebrin in cultured cortical neurons
J Neurosci
1999
19(10):3918-25
PubMed ID: 10234022
DOI: 10.1523/JNEUROSCI.19-10-03918.1999
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2149
Hayashi K, Ishikawa R, Kawai-Hirai R, Takagi T, Taketomi A, Shirao T.
Domain analysis of the actin-binding and actin-remodeling activities of drebrin
Exp Cell Res
1999
253(2):673-80
PubMed ID: 10585290
DOI: 10.1006/excr.1999.4663
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