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Cell No. : Cell Name
RCB2309 : JFP-J1  update : 2024/01/17
CommentHybridoma producing monoclonal antibody against GFP. Rat IgG2a monoclonal antibody.
Comment from the depositor
Terms and conditions1) Material is considered the proprietary property of Mitsubishi Kagaku Institute of Life Science (MITILA) and Mitsubishi Chemical Corporation (MCC) . 2) Recipient Investigator agrees not to use Material for profit making or commercial purposes. 3) Recipient Investigator shall acknowledge the source of Material in any publication containing experimental results obtained with the use of Material. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Neurochem 1999;19:3918-3925) designated by the DEPOSITOR is required. 5) At the conclusion of the research for which Material was requested, Recipient shall dispose of unused Material in an appropriate legal manner. 6) After providing Material, RIKEN BRC shall inform MCC of followings;names of Recipient and Recipient Investigator, purpose and date.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takayama, Hajime
Originator Fujita, Shinobu
Year of deposit 2006
Another name 851D7
Animal _mouse x rat < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_C478
hybridoma Fusion_cell_1 name P3-X63-Ag8.653
Fusion_cell_1 strain mouse
Fusion_cell_2 name spleen cell
Fusion_cell_2 strain rat, Wistar
Fusion_cell_2 gender Female
Fusion method PEG
Antibody name JFP-J1
Subclass IgG2a
Immunogen recombinant GFP
Antigen_name GFP
Antigen_m.w. 30kDa
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2a
deposit info
lot info
Reference information Reference 1
User's Publication 3

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3615      J Neurochem  1999  19:3918-392   

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User's Publication
7078  Hasegawa K, Yasuda T, Shiraishi C, Fujiwara K, Przedborski S, Mochizuki H, Yoshikawa K.  Promotion of mitochondrial biogenesis by necdin protects neurons against mitochondrial insults.  Nat Commun  2016  7:10943  PubMed ID: 26971449   DOI: 10.1038/ncomms10943
14312  Nagai T, Furusho Y, Li H, Hasui K, Matsukita S, Sueyoshi K, Yanagi M, Hatae M, Takao S, Matsuyama T.  Production of a High-affinity Monoclonal Antibody Reactive with Folate Receptors Alpha and Beta  Monoclon Antib Immunodiagn Immunother  2015  34(3):181-90  PubMed ID: 26090596   DOI: 10.1089/mab.2014.0072
18237  Mazzolini R, Rodrigues P, Bazzocco S, Dopeso H, Ferreira AM, Mateo-Lozano S, Andretta E, Woerner SM, Alazzouzi H, Landolfi S, Hernandez-Losa J, Macaya I, Suzuki H, Ramón y Cajal S, Mooseker MS, Mariadason JM, Gebert J, Hofstra RM, Reventós J, Yamamoto H, Schwartz S Jr, Arango D.  Brush border myosin Ia inactivation in gastric but not endometrial tumors  Int J Cancer  2013  132(8):1790-9  PubMed ID: 23002058   DOI: 10.1002/ijc.27856

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