Cell No. : Cell Name
RCB2308 : 802C11
update : 2025/01/16
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| Comment | Hybridoma producing monoclonal antibody against MuSc. |
| Comment from the depositor | |
| Terms and conditions | 1) Material is considered the proprietary property of Mitsubishi Kagaku Institute of Life Science (MITILA) and Mitsubishi Chemical Corporation (MCC) . 2) Recipient Investigator agrees not to use Material for profit making or commercial purposes. 3) Recipient Investigator shall acknowledge the source of Material in any publication containing experimental results obtained with the use of Material. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Neurochem 1998;80:2810-2824) designated by the DEPOSITOR is required. 5) At the conclusion of the research for which Material was requested, Recipient shall dispose of unused Material in an appropriate legal manner. 6) After providing Material, RIKEN BRC shall inform MCC of followings;names of Recipient and Recipient Investigator, purpose and date. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Takayama, Hajime
|
| Originator |
Fujita, Shinobu
|
| Year of deposit |
2006
|
| Animal |
_mouse x hamster
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_C475
|
|
hybridoma
|
Fusion_cell_1 name |
spleen cell
|
| Fusion_cell_1 strain |
hamster, Armenian
|
| Fusion_cell_2 name |
P3-X63-Ag8.653
|
| Fusion_cell_2 strain |
mouse
|
|
Fusion method |
PEG
|
|
Antibody name |
802C11
|
| Subclass |
IgG
|
| Immunogen |
embryonic(E12.5) mouse(ICR ) brain
|
|
Antigen_name |
MuSc
|
| Antigen_m.w. |
95〜100kDa
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
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| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
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Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Antibody subclass |
|
IgG
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
2222
Dillon AK, Fujita SC, Matise MP, Jarjour AA, Kennedy TE, Kollmus H, Arnold HH, Weiner JA, Sanes JR, Kaprielian Z.
Molecular control of spinal accessory motor neuron/axon development in the mouse spinal cord
J Neurosci
2005
25(44):10119-30
PubMed ID: 16267219
DOI: 10.1523/JNEUROSCI.3455-05.2005
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2221
awauchi D, Kobayashi H, Sekine-Aizawa Y, Fujita SC, Murakami F.
MuSC is involved in regulating axonal fasciculation of mouse primary vestibular afferents
Eur J Neurosci
2003
18(8):2244-52
PubMed ID: 14622185
DOI: 10.1046/j.1460-9568.2003.02968.x
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3614
Sekine-Aizawa Y, Omori A, Fujita SC.
MuSC, a novel member of the immunoglobulin superfamily, is expressed in neurons of a subset of cranial sensory ganglia in the mouse embryo
Eur J Neurosci
1998
80:2810-2824
PubMed ID: 9758151
DOI: 10.1111/j.1460-9568.1998.00288.x
|
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