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Cell No. : Cell Name
RCB2307 : T1.7  update : 2021/05/20
CommentHybridoma producing monoclonal antibody against C-terminal peptide of rat GSK3-beta.
Comment from the depositor
Terms and conditions1) Material is considered the proprietary property of Mitsubishi Kagaku Institute of Life Science (MITILA) and Mitsubishi Chemical Corporation (MCC) . 2) Recipient Investigator agrees not to use Material for profit making or commercial purposes. 3) Recipient Investigator shall acknowledge the source of Material in any publication containing experimental results obtained with the use of Material. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Neurochem 2002;81:1073-1083) designated by the DEPOSITOR is required. 5) At the conclusion of the research for which Material was requested, Recipient shall dispose of unused Material in an appropriate legal manner. 6) After providing Material, RIKEN BRC shall inform MCC of followings;names of Recipient and Recipient Investigator, purpose and date.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Takayama, Hajime
Originator Fujita, Shinobu
Year of deposit 2006
Another name 777C5
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_K255
hybridoma Fusion_cell_1 name P3-X63-Ag8.653
Fusion_cell_1 strain mouse
Fusion_cell_2 name spleen cell
Fusion_cell_2 strain mouse, BALB/c
Fusion_cell_2 gender Female
Fusion method PEG
Antibody name T1.7
Subclass IgG1
Immunogen C-terminal peptide of rat GSK3β
Antigen_name GSK3β
Antigen_m.w. 45kDa
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG1
Reference information Reference 1
User's Publication 0


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Reference
3613  Mukai F, Ishiguro K, Sano Y, Fujita SC.  Alternative splicing isoform of tau protein kinase I/glycogen synthase kinase 3beta.  J Neurochem  2002  81(5):1073-83  PubMed ID: 12065620   DOI: 10.1046/j.1471-4159.2002.00918.x

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