Cell No. : Cell Name
RCB2306 : PC3.1
update : 2025/02/26
|
| Comment | Hybridoma producing monoclonal antibody against latexin. |
| Comment from the depositor | |
| Terms and conditions | 1) Material is considered the proprietary property of Mitsubishi Kagaku Institute of Life Science (MITILA) and Mitsubishi Chemical Corporation (MCC) . 2) Recipient Investigator agrees not to use Material for profit making or commercial purposes. 3) Recipient Investigator shall acknowledge the source of Material in any publication containing experimental results obtained with the use of Material. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Proc Natl Acad Sci USA 1992;89:8879-8883) designated by the DEPOSITOR is required. 5) At the conclusion of the research for which Material was requested, Recipient shall dispose of unused Material in an appropriate legal manner. 6) After providing Material, RIKEN BRC shall inform MCC of followings;names of Recipient and Recipient Investigator, purpose and date. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Takayama, Hajime
|
| Originator |
Arimatsu, Yasuyoshi
|
| Year of deposit |
2006
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_U140
|
|
hybridoma
|
Fusion_cell_1 name |
P3-X63-Ag8.653
|
| Fusion_cell_1 strain |
mouse
|
| Fusion_cell_2 name |
spleen cell
|
| Fusion_cell_2 strain |
mouse, BALB/c
|
| Fusion_cell_2 gender |
Female
|
|
Fusion method |
PEG, HAT selection
|
|
Antibody name |
PC3.1
|
| Subclass |
IgG1
|
| Immunogen |
Brain(parietal and temporal region) of rat
|
|
Antigen_name |
latexin
|
| Antigen_m.w. |
29kDa
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Antibody subclass |
|
IgG1
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
9
|
| User's Publication |
0
|
| Reference |
|
3515
Arimatsu Y, Nihonmatsu I, Hatanaka Y.
Localization of latexin-immunoreactive neurons in the adult cat cerebral cortex and claustrum/endopiriform formation.
Neuroscience
2009
162(4):1398-410
PubMed ID: 19486926
DOI: 10.1016/j.neuroscience.2009.05.060
|
|
2224
Bai WZ, Ishida M, Arimatsu Y.
Chemically defined feedback connections from infragranular layers of sensory association cortices in the rat
Neuroscience
2004
123(1):257-67
PubMed ID: 14667460
DOI: 10.1016/j.neuroscience.2003.08.056
|
|
2229
Arimatsu Y, Kojima M, Ishida M.
Area- and lamina-specific organization of a neuronal subpopulation defined by expression of latexin in the rat cerebral cortex
Neuroscience
1999
88(1):93-105
PubMed ID: 10051192
DOI: 10.1016/s0306-4522(98)00185-7
|
|
2230
Arimatsu Y, Ishida M, Takiguchi-Hayashi K, Uratani Y.
Cerebral cortical specification by early potential restriction of progenitor cells and later phenotype control of postmitotic neurons
Development
1999
126(4):629-38
PubMed ID: 9895311
DOI: 10.1242/dev.126.4.629
|
|
2228
Arimatsu Y, Ishida M.
Early patterning of the rat cerebral wall for regional organization of a neuronal population expressing latexin
Brain Res Dev Brain Res
1998
106(1-2):71-8
PubMed ID: 9554959
DOI: 10.1016/s0165-3806(97)00197-1
|
|
2227
Takiguchi-Hayashi K, Arimatsu Y.
Restricted expression of latexin in dorsal midline cells of developing rat forebrain
Neuroreport
1995
6(2):281-3
PubMed ID: 7756610
DOI: 10.1097/00001756-199501000-00014
|
|
2225
Arimatsu Y, Nihonmatsu I, Hirata K, Takiguchi-Hayashi K.
Cogeneration of neurons with a unique molecular phenotype in layers V and VI of widespread lateral neocortical areas in the rat
J Neurosci
1994
14(4):2020-31
PubMed ID: 8158255
DOI: 10.1523/JNEUROSCI.14-04-02020.1994
|
|
2226
Hatanaka Y, Uratani Y, Takiguchi-Hayashi K, Omori A, Sato K, Miyamoto M, Arimatsu Y.
Intracortical regionality represented by specific transcription for a novel protein, latexin
Eur J Neurosci
1994
6(6):973-82
PubMed ID: 7524963
DOI: 10.1111/j.1460-9568.1994.tb00592.x
|
|
3612
Arimatsu Y, Miyamoto M, Nihonmatsu I, Hirata K, Uratani Y, Hatanaka Y, Takiguchi-Hayashi K.
Early regional specification for a molecular neuronal phenotype in the rat neocortex.
Proc Natl Acad Sci U S A
1992
89(19):8879-83
PubMed ID: 1409580
DOI: 10.1073/pnas.89.19.8879
|
Back
Top
CELL BANK Website
