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Cell No. : Cell Name
RCB2305 : MIH5(GIT)  update : 2022/02/10
CommentHybridoma producing a monoclonal antibody against mouse B7-H1.
Comment from the depositor
Terms and conditions1) The RECIOIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent from the DEPOCITOR. 2) The RECIPIENT shall be a non-profit entity only. 3) The BIOLOGICAL RESOURCE shall be used for teaching or not-for-profit research purposes only. 4) In publishing the research results obtained by the use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR’s SCIENTIST is requested. Also the RECIPIENT agree to acknowledgment to the DEPOSITOR’s SCIENTIST as a coauthor in such publication that the BIOLOGICAL RESOURCE materially contributes to the publications. 5) if the RECIPIENT discovers creates or derives new material or invention though the use of the BIOLOGICAL RESOURCE, the ownership of any patent or other intellectual property right to the material or invention shall be negotiated between PROVIDER and RECIPIENT
approver's address
Graduate School, Tokyo Medical and Dental University

1-5-45, Yushima, Bunkyo-ku, Tokyo Japan
Dr.AZUMA Miyuki
Fax. +81-3-5803-5935
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Azuma, Miyuki
Originator Azuma, Miyuki
Year of deposit 2006
Animal mouse x rat < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_C663
hybridoma Fusion_cell_1 name P3U1
Fusion_cell_1 strain mouse
Fusion_cell_2 name spleen
Fusion_cell_2 strain rat, SD
Fusion_cell_2 gender Female
Fusion method PEG, HAT selection
Antibody name MIH5
Subclass IgG2a
Immunogen mouse B7-H1 transfected L5178Y
Antigen_name B7-H1/PD-L1
Antigen_character mouse B7-H1
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives GIT
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium GIT : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2a
deposit info
lot info
Reference information Reference 3
User's Publication 0

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3458  Tsushima, Fumihiko, Tanaka, Kae, Otsuki, Noriko, Youngnak, Pornpan, Iwai, Hideyuki, Omura, Ken, Azuma, Miyuki  Predominant expression of B7-H1 and its immunoregulatory roles in oral squamous cell carcinoma.  Oral Oncol  2006  42:268-74  PubMed ID: 16271509  
3457  Tsushima, Fumihiko, Iwai, Hideyuki, Otsuki, Noriko, Abe, Masaaki, Hirose, Sachiko, Yamazaki, Tomohide, Akiba, Hisaya, Yagita, Hideo, Takahashi, Yuzo, Omura, Ken, Okumura, Ko, Azuma, Miyuki  Preferential contribution of B7-H1 to programmed death-1-mediated regulation of hapten-specific allergic inflammatory responses.  Eur J Immunol  2003  33:2773-82  PubMed ID: 14515261   DOI: 10.1002/eji.200324084
3456  Yamazaki, Tomohide, Akiba, Hisaya, Iwai, Hideyuki, Matsuda, Hironori, Aoki, Mami, Tanno, Yuka, Shin, Tahiro, Tsuchiya, Haruo, Pardoll, Drew M, Okumura, Ko, Azuma, Miyuki, Yagita, Hideo  Expression of programmed death 1 ligands by murine T cells and APC.  J Immunol  2002  169:5538-45  PubMed ID: 12421930   DOI: 10.4049/jimmunol.169.10.5538

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