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Cell No. : Cell Name
RCB2302 : RMC-6  update : 2023/10/16
CommentRat cell line derived from mammary gland carcinoma which is induced by DMBA in the transgenic rat expressing human prototype c-Ha-ras gene.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Nagoya City University

1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan
Dr.TSUDA Hiroyuki
Fax. +81-52-853-8996
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Tsuda, Hiroyuki
Originator Tsuda, Hiroyuki
Year of deposit 2006
Another name C6
Animal _rat < Mammals
Strain name c-Ha-ras transgenic
Gender Female
Tissue mammary gland
Disease name mammary carcinoma
Classification cancer
Recombinant recombinant
Exogene human c-Ha-ras genomic DNA
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_5359
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD, AST
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
10106  Nohmi T, Masumura K, Toyoda-Hokaiwado N.  Transgenic rat models for mutagenesis and carcinogenesis.  Genes Environ  2017  39:11  PubMed ID: 28174618   DOI: 10.1186/s41021-016-0072-6

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