Cell No. : Cell Name
RCB2297 : PKCβ^(-)DT40
update : 2024/01/17
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| Comment | Subline of DT40 cell line, lacking PKC-β expression. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Exp Med 2005;202:1423-31) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Kurosaki, Tomohiro
|
| Originator |
Kurosaki, Tomohiro
|
| Year of deposit |
2006
|
| Another name |
K143-8
|
| Original cell |
DT40
|
| Animal |
_avian
< Birds
|
| Tissue |
B cell
|
| Classification |
mutant
|
| Recombinant |
recombinant
|
| Exogene |
lacking PKC-β
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_1T42
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
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| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
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Culture information
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Passage cell No |
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1.5-2 x 10 5 cells/ml
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| Passage ratio |
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1 : 20 split
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| SC frequency |
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Subculture : every 2 days
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| Temperature |
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39.5
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
90% FBS + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
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| Others |
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Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
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| Reference information |
Reference |
1
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| User's Publication |
0
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