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Cell No. : Cell Name
RCB2295 : KUM10  update : 2020/09/24
CommentMouse mesenchymal cell line derived from bone marrow. C57/B6.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2006
Animal mouse < Mammals
Strain name C57/B6
Tissue bone marrow
Classification normal
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives POWEREDBY10
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage cell No 2-3 x 10^(5) cells/100 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 0
User's Publication 2


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Reference

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User's Publication
10781  Itoh H, Kadomatsu T, Tanoue H, Yugami M, Miyata K, Endo M, Morinaga J, Kobayashi E, Miyamoto T, Kurahashi R, Terada K, Mizuta H, Oike Y.  TET2-dependent IL-6 induction mediated by the tumor microenvironment promotes tumor metastasis in osteosarcoma.  Oncogene  2018  37(22):2903-2920  PubMed ID: 29515232
9822  Makiguchi T, Yamada M, Yoshioka Y, Sugiura H, Koarai A, Chiba S, Fujino N, Tojo Y, Ota C, Kubo H, Kobayashi S, Yanai M, Shimura S, Ochiya T, Ichinose M.  Serum extracellular vesicular miR-21-5p is a predictor of the prognosis in idiopathic pulmonary fibrosis.  Respir. Res.  2016  17:110  PubMed ID: 27596748



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