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Cell No. : Cell Name
RCB2295 : KUM10  update : 2023/10/16
CommentMouse mesenchymal cell line derived from bone marrow. C57/B6.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2006
Animal _mouse < Mammals
Strain name C57/B6
Tissue bone marrow
Classification normal
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5148
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium POWEREDBY10
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 2-3 x 10 5 cells/100 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 0
User's Publication 6

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User's Publication
17699  Kuroda A, Mineo A, Shoji S, Inoue G, Saito W, Sekiguchi H, Takaso M, Uchida K.  Effect of spheroid size on gene expression profiles of a mouse mesenchymal stem cell line in spheroid culture  Biomed Mater Eng  2023  34(1):67-76  PubMed ID: 35694914   DOI: 10.3233/BME-221406
10781  Itoh H, Kadomatsu T, Tanoue H, Yugami M, Miyata K, Endo M, Morinaga J, Kobayashi E, Miyamoto T, Kurahashi R, Terada K, Mizuta H, Oike Y.  TET2-dependent IL-6 induction mediated by the tumor microenvironment promotes tumor metastasis in osteosarcoma.  Oncogene  2018  37(22):2903-2920  PubMed ID: 29515232   DOI: 10.1038/s41388-018-0160-0
9680  Suto N, Mieda T, Iizuka A, Nakamura K, Hirai H.  Morphological and Functional Attenuation of Degeneration of Peripheral Neurons by Mesenchymal Stem Cell-Conditioned Medium in Spinocerebellar Ataxia Type 1-Knock-in Mice.  CNS Neurosci Ther  2016  22:670-6  PubMed ID: 27140210   DOI: 10.1111/cns.12560
9822  Makiguchi T, Yamada M, Yoshioka Y, Sugiura H, Koarai A, Chiba S, Fujino N, Tojo Y, Ota C, Kubo H, Kobayashi S, Yanai M, Shimura S, Ochiya T, Ichinose M.  Serum extracellular vesicular miR-21-5p is a predictor of the prognosis in idiopathic pulmonary fibrosis.  Respir. Res.  2016  17:110  PubMed ID: 27596748   DOI: 10.1186/s12931-016-0427-3
14100  Mieda T, Suto N, Iizuka A, Matsuura S, Iizuka H, Takagishi K, Nakamura K, Hirai H.  Mesenchymal stem cells attenuate peripheral neuronal degeneration in spinocerebellar ataxia type 1 knockin mice  J Neurosci Res  2016  94(3):246-52  PubMed ID: 26707550   DOI: 10.1002/jnr.23698
15075  Ono M, Ohkouchi S, Kanehira M, Tode N, Kobayashi M, Ebina M, Nukiwa T, Irokawa T, Ogawa H, Akaike T, Okada Y, Kurosawa H, Kikuchi T, Ichinose M.  Mesenchymal stem cells correct inappropriate epithelial-mesenchyme relation in pulmonary fibrosis using stanniocalcin-1  Mol Ther  2015  23(3):549-60  PubMed ID: 25373521   DOI: 10.1038/mt.2014.217

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