Cell No. : Cell Name
RCB2295 : KUM10
update : 2024/08/14
|
| Comment | Mouse mesenchymal cell line derived from bone marrow. C57/B6. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Umezawa, Akihiro
|
| Originator |
Umezawa, Akihiro
|
| Year of deposit |
2006
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C57/B6
|
| Tissue |
bone marrow
|
| Classification |
normal
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_5148
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
POWEREDBY10
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage cell No |
|
2-3 x 10 5 cells/100 mm dish
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
0
|
| User's Publication |
7
|
| User's Publication |
|
22114
Takeda K, Saito H, Shoji S, Sekiguchi H, Matsumoto M, Ujihira M, Miyagi M, Inoue G, Takaso M, Uchida K.
Enhancing Bone Formation Through bFGF-Loaded Mesenchymal Stromal Cell Spheroids During Fracture Healing in Mice.
Bioengineering (Basel)
2024
11(10)
PubMed ID: 39451415
DOI: 10.3390/bioengineering11101041
|
|
17699
Kuroda A, Mineo A, Shoji S, Inoue G, Saito W, Sekiguchi H, Takaso M, Uchida K.
Effect of spheroid size on gene expression profiles of a mouse mesenchymal stem cell line in spheroid culture
Biomed Mater Eng
2023
34(1):67-76
PubMed ID: 35694914
DOI: 10.3233/BME-221406
|
|
10781
Itoh H, Kadomatsu T, Tanoue H, Yugami M, Miyata K, Endo M, Morinaga J, Kobayashi E, Miyamoto T, Kurahashi R, Terada K, Mizuta H, Oike Y.
TET2-dependent IL-6 induction mediated by the tumor microenvironment promotes tumor metastasis in osteosarcoma.
Oncogene
2018
37(22):2903-2920
PubMed ID: 29515232
DOI: 10.1038/s41388-018-0160-0
|
|
9680
Suto N, Mieda T, Iizuka A, Nakamura K, Hirai H.
Morphological and Functional Attenuation of Degeneration of Peripheral Neurons by Mesenchymal Stem Cell-Conditioned Medium in Spinocerebellar Ataxia Type 1-Knock-in Mice.
CNS Neurosci Ther
2016
22:670-6
PubMed ID: 27140210
DOI: 10.1111/cns.12560
|
|
9822
Makiguchi T, Yamada M, Yoshioka Y, Sugiura H, Koarai A, Chiba S, Fujino N, Tojo Y, Ota C, Kubo H, Kobayashi S, Yanai M, Shimura S, Ochiya T, Ichinose M.
Serum extracellular vesicular miR-21-5p is a predictor of the prognosis in idiopathic pulmonary fibrosis.
Respir. Res.
2016
17:110
PubMed ID: 27596748
DOI: 10.1186/s12931-016-0427-3
|
|
14100
Mieda T, Suto N, Iizuka A, Matsuura S, Iizuka H, Takagishi K, Nakamura K, Hirai H.
Mesenchymal stem cells attenuate peripheral neuronal degeneration in spinocerebellar ataxia type 1 knockin mice
J Neurosci Res
2016
94(3):246-52
PubMed ID: 26707550
DOI: 10.1002/jnr.23698
|
|
15075
Ono M, Ohkouchi S, Kanehira M, Tode N, Kobayashi M, Ebina M, Nukiwa T, Irokawa T, Ogawa H, Akaike T, Okada Y, Kurosawa H, Kikuchi T, Ichinose M.
Mesenchymal stem cells correct inappropriate epithelial-mesenchyme relation in pulmonary fibrosis using stanniocalcin-1
Mol Ther
2015
23(3):549-60
PubMed ID: 25373521
DOI: 10.1038/mt.2014.217
|
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