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Cell No. : Cell Name
RCB2283 : 277-LCL  update : 2024/01/17
CommentHuman B cells transformed by Epstein-Barr virus. TKG0609 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Kudo, T.
Year of deposit 2006
Original cell TKG0609
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue peripheral blood
Classification transformed
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0609)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_8073
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
7788  Yamaguchi M, Fujimura K, Kanegane H, Toga-Yamaguchi H, Chopra R, Okamura N.  Mislocalization or low expression of mutated Shwachman-Bodian-Diamond syndrome protein.  Int. J. Hematol.  2011  94:54-62  PubMed ID: 21660439   DOI: 10.1007/s12185-011-0880-1



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