Cell No. : Cell Name RCB2283 : 277-LCL  update : 2024/01/17
Comment			Human B cells transformed by Epstein-Barr virus. TKG0609 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Obinata, Masuo
		Originator	Kudo, T.
		Year of deposit	2006
		Original cell	TKG0609
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Tissue	peripheral blood
		Classification	transformed
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0609）
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_8073
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Virus (HIV)		LT 400
		Virus (HTLV-1)		(-)
		Isozyme		LD, NP
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	0
		User's Publication	2

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Reference

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User's Publication

21670  Motonari T, Yoshino Y, Haruta M, Endo S, Sasaki S, Miyashita M, Tada H, Watanabe G, Kaneko T, Ishida T, Chiba N.  Evaluating homologous recombination activity in tissues to predict the risk of hereditary breast and ovarian cancer and olaparib sensitivity.  Sci Rep  2024  14(1):7519  PubMed ID: 38589490   DOI: 10.1038/s41598-024-57367-6

7788  Yamaguchi M, Fujimura K, Kanegane H, Toga-Yamaguchi H, Chopra R, Okamura N.  Mislocalization or low expression of mutated Shwachman-Bodian-Diamond syndrome protein.  Int. J. Hematol.  2011  94:54-62  PubMed ID: 21660439   DOI: 10.1007/s12185-011-0880-1