Cell No. : Cell Name RCB2281 : T3M-12  update : 2021/02/26
Comment			Human lung small cell carcinoma cell line. ADH producing.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
	Basic information	Depositor	Okabe, Tetsuro
		Originator	Okabe, Tetsuro
		Year of deposit	2005
		Another name	ADH
		Animal	human < Mammals
		Genus	Homo
		Species	sapiens
		Tissue	lung
		Disease name	small cell carcinoma
		Classification	cancer
		Lifespan	infinite
		Morphology	other
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Medium and additives		HamF10 + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 2-4 split
		SC frequency		Subculture : once/week, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		STR(human)		OK
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	0
		User's Publication	2

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Reference

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User's Publication

9502  Morita M, Sato T, Nomura M, Sakamoto Y, Inoue Y, Tanaka R, Ito S, Kurosawa K, Yamaguchi K, Sugiura Y, Takizaki H, Yamashita Y, Katakura R, Sato I, Kawai M, Okada Y, Watanabe H, Kondoh G, Matsumoto S, Kishimoto A, Obata M, Matsumoto M, Fukuhara T, Motohashi H, Suematsu M, Komatsu M, Nakayama KI, Watanabe T, Soga T, Shima H, Maemondo M, Tanuma N.  PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth.  Cancer Cell  2018  33:355-367.e7  PubMed ID: 29533781

6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309