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Cell No. : Cell Name
RCB2280 : HCE-T  update : 2020/09/28
CommentSV40-Adeno vector transformed cornea cells. 64kD keratin producing. Choleratoxin-free medium. Caution! PDF 91KB
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Invest Ophthalmol Vis Sci. 1995;36:614-621)
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Araki-Sasaki Kaoru
Originator Araki-Sasaki Kaoru
Year of deposit 2005
Original cell RCB1384
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 49 years
Tissue cornea
Case history maxillary sinus carcinoma
Classification transformed
Recombinant recombinant
Exogene SV40-large T
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 5% FBS + 5μg/ml Insulin + 10ng/ml human EGF + 0.5% DMSO
Antibiotics Free
Passage method 0.25% trypsin or (0.25% trypsin + 0.02% EDTA)
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 5
User's Publication 118

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9981  Araki-Sasaki K, Aizawa S, Hiramoto M, Nakamura M, Iwase O, Nakata K, Sasaki Y, Mano T, Handa H, Tano Y.  Substance P-induced cadherin expression and its signal transduction in a cloned human corneal epithelial cell line.  J Cell Physiol  2000  182(2):189-95  PubMed ID: 10623882
9980  Saarinen-Savolainen P, Järvinen T, Araki-Sasaki K, Watanabe H, Urtti A. Author information  Evaluation of cytotoxicity of various ophthalmic drugs, eye drop excipients and cyclodextrins in an immortalized human corneal epithelial cell line.  Pharm Res  1998  15(8):1275-80  PubMed ID: 9706061
2263  Araki-Sasaki K, Ohashi Y, Sasabe T, Hayashi K, Watanabe H, Tano Y, Handa H  An SV40-immortalized human corneal epithelial cell line and its characterization.  Invest Ophthalmol Vis Sci  1995  36:614-21  PubMed ID: 7534282
2262  Zhang Y, Araki-Sasaki K, Handa H, Akhtar, R A  Effect of carbachol on phospholipase C-mediated phosphatidylinositol 4,5-bisphosphate hydrolysis, and its modulation by isoproterenol in rabbit corneal epithelial cells.  Curr Eye Res  1995  14:563-71  PubMed ID: 7587302
2261  Araki K, Ohashi Y, Sasabe T, Kinoshita S, Hayashi K, Yang, X Z, Hosaka Y, Aizawa S, Handa H  Immortalization of rabbit corneal epithelial cells by a recombinant SV40-adenovirus vector.  Invest Ophthalmol Vis Sci  1993  34:2665-71  PubMed ID: 7688358

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User's Publication
6967  Seino S1,, Matsuoka R, Masuda Y, Kunou M, Okada Y, Saika S.  Topical hyaluronan alone promotes corneal epithelial cell migration whereas combination with benzalkonium chloride impairs epithelial wound healing.  Cutan Ocul Toxicol  2020  39(1):13-20  PubMed ID: 31588814
11893  Vitoux MA, Kessal K, Melik Parsadaniantz S, Claret M, Guerin C, Baudouin C, Brignole-Baudouin F, Réaux-Le Goazigo A.  Benzalkonium chloride-induced direct and indirect toxicity on corneal epithelial and trigeminal neuronal cells: proinflammatory and apoptotic responses in vitro.  Toxicol. Lett.  2020    PubMed ID: 31707104
11963  Jurišić Dukovski B, Juretić M, Bračko D, Randjelović D, Savić S, Crespo Moral M, Diebold Y, Filipović-Grčić J, Pepić I, Lovrić J.  Functional ibuprofen-loaded cationic nanoemulsion: Development and optimization for dry eye disease treatment.  Int J Pharm  2020    PubMed ID: 31870964
11983  Zeng W, Zhang Y, Duan F, Lin T, Liu X, Li D, Wu K.  Lipopolysaccharide enhances human herpesvirus 1 replication and IL-6 release in epithelial cells.  Microb. Pathog.  2020    PubMed ID: 31904451
11998  Rubelowski AK, Latta L, Katiyar P, Stachon T, Käsmann-Kellner B, Seitz B, Szentmáry N.  HCE-T cell line lacks cornea-specific differentiation markers compared to primary limbal epithelial cells and differentiated corneal epithelium.  Graefes Arch. Clin. Exp. Ophthalmol.  2020    PubMed ID: 31927639
12024  Fu R, Guo H, Janga S, Choi M, Klinngam W, Edman MC, Hamm-Alvarez SF.  Cathepsin S activation contributes to elevated CX3CL1 (fractalkine) levels in tears of a Sjögren's syndrome murine model.  Sci Rep  2020    PubMed ID: 31996771
12145  Davood Kharaghani, Debarun Dutta, Kitty K. K. Ho, Ke-Qin Zhang, Wei Kai, Xuehong Ren, Mark D. P. Willcox & Ick Soo Kim  Active loading graphite/hydroxyapatite into the stable hydroxyethyl cellulose scaffold nanofibers for artificial cornea application  Original Research  2020   
12173  Pierre-Louis Destruel,Ni Zeng, Françoise Brignole-Baudouin,Sophie Douat, Johanne Seguin, Elodie Olivier, Melody Dutot, ,Patrice Rat, Sophie Dufaÿ, Amélie Dufaÿ-Wojcicki, Marc Maury, Nathalie Mignet, * andVincent Boudy,   In Situ Gelling Ophthalmic Drug Delivery System for the Optimization of Diagnostic and Preoperative Mydriasis: In Vitro Drug Release, Cytotoxicity and Mydriasis Pharmacodynamics  Pharmaceutics  2020    PubMed ID: 32326492
11556  Fuwa M, Shimazaki A, Mieda M, Yamashita N, Akaishi T, Taniguchi T, Kato M.  Efficacy and safety of the fixed combinations of tafluprost/timolol and latanoprost/carteolol.  Sci Rep  2019    PubMed ID: 31097790
11595  Koyama S, Narita E, Suzuki Y, Shiina T, Taki M, Shinohara N, Miyakoshi J.  Long-term exposure to a 40-GHz electromagnetic field does not affect genotoxicity or heat shock protein expression in HCE-T or SRA01/04 cells.  J. Radiat. Res.  2019    PubMed ID: 31197350
11636  Kawata K, Aoki S, Futamata M, Yamamoto-Rikitake M, Nakao I, Enaida H, Toda S.  Mesenchymal cells and fluid flow stimulation synergistically regulate the kinetics of corneal epithelial cells at the air-liquid interface.  Graefes Arch. Clin. Exp. Ophthalmol.  2019    PubMed ID: 31321523
11661  Panita Suwannoi, Mullika Chomnawang, Amolnat Tunsirikongkon, Angsuma Phongphisutthinan, Christel C.Müller-Goymann, Narong Sarisuta  TAT-surface modified acyclovir-loaded albumin nanoparticles as a novel ocular drug delivery system  Journal of Drug Delivery Science and Technology  2019   
11818  Dukovski BJ, Bračko A, Šare M, Pepić I, Lovrić J.  In vitro evaluation of stearylamine cationic nanoemulsions for improved ocular drug delivery.  Acta Pharm  2019    PubMed ID: 31639085
6948  Tong L, Matsuura E, Takahashi M, Nagano T, Kawazu K.  Effects of Anti-Glaucoma Prostaglandin Ophthalmic Solutions on Cultured Human Corneal Epithelial Cells.  Curr Eye Res  2019  44(8):856-862  PubMed ID: 30884982
6970  Kazuyo Sadamoto, Shingo Nemoto, Akio Kawamura, Masaaki Kurata  Comparison of cytotoxicity between fixed- and unfixed-combination ophthalmic solutions in vitro  Fundamental Toxicological Sciences  2019  6:245-248 
9139  Guoliang Wang, Yuhua Xue, Yanzi Wang, Fei Dong, Mei Shen, Rongrong Zong, Zuguo Liu, Cheng Li   The Role of Autophagy in the Pathogenesis of Exposure Keratitis  J Cell Mol Med  2019  23 (6), 4217-4228  PubMed ID: 30973208
10688  Noriko Yaekashiwa, Hisa Yoshida, Sato Otsuki, Shin’ichiro Hayashi and Kodo Kawase  Verification of Non-thermal Effects of 0.3–0.6 THz-Waves on Human Cultured Cells  Photonics  2019  6(1):33 
11956  L Kiss E, Berkó S, Gácsi A, Kovács A, Katona G, Soós J, Csányi E, Gróf I, Harazin A, Deli MA, Budai-Szűcs M.  Design and Optimization of Nanostructured Lipid Carrier Containing Dexamethasone for Ophthalmic Use.  Pharmaceutics  2019    PubMed ID: 31847336
10627  Beißner N, Zorn-Kruppa M, Reichl S.  Parameter study of shipping conditions for the ready-to-use application of a 3D human hemicornea construct in drug absorption studies.  Int J Pharm  2018  536:377-387  PubMed ID: 29191484
10824  Juretić M, Cetina-Čižmek B, Filipović-Grčić J, Hafner A, Lovrić J, Pepić I.  Biopharmaceutical evaluation of surface active ophthalmic excipients using in vitro and ex vivo corneal models.  Eur J Pharm Sci  2018  120:133-141  PubMed ID: 29702232
11118  Yin Y, Zong R, Bao X, Zheng X, Cui H, Liu Z, Zhou Y.  Oxidative Stress Suppresses Cellular Autophagy in Corneal Epithelium.  Invest. Ophthalmol. Vis. Sci.  2018  59(8):3286-3293  PubMed ID: 29971448
11136  Gillies PJ, Richardson NA, Walshe J, Stephenson SA, Dawson RA, Harkin DG.  Demonstration of P-selectin expression and potential function in human corneal epithelial cells.  Exp. Eye Res.  2018  176:196-206  PubMed ID: 30025918
11142  Marek V, Melik-Parsadaniantz S, Villette T, Montoya F, Baudouin C, Brignole-Baudouin F, Denoyer A.  Blue light phototoxicity toward human corneal and conjunctival epithelial cells in basal and hyperosmolar conditions.  Free Radic. Biol. Med.  2018  126:27-40  PubMed ID: 30040995
11175  Png E, Hou A, Tong L.  Mechanistic role of transglutaminase-2 in focal adhesions.  Sci Rep  2018  8(1):12370  PubMed ID: 30120307
11291  von Deylen D, Dreher C, Seidelmann O, Reichl S.  New Classes of Polycationic Compounds as Preservatives for Ophthalmic Formulations.  Pharm. Res.  2018    PubMed ID: 30411196
11370  Xu P, Kai C, Kawasaki S, Kobayashi Y, Yamamoto K, Tsujikawa M, Hayashi R, Nishida K.  A New in Vitro Model of GDLD by Knocking Out TACSTD2 and Its Paralogous Gene EpCAM in Human Corneal Epithelial Cells.  Transl Vis Sci Technol  2018    PubMed ID: 30619650
4084  Akiko TOJU, Yuko UDAKA, Mayumi TSUJI, Hideto OYAMADA, Junichiro KIZAKI, Eiji TOMOYORI, Yuji KIUCHI  Suppressive Effects of Catechins in UV-Induced Cytotoxicity of Human Corneal Epithelial Cells  Showa Univ J Med Sci  2018  3, 73~85 
4191  Krtalić I, Radošević S, Hafner A, Grassi M, Nenadić M, Cetina-Čižmek B, Filipović-Grčić J, Pepić I, Lovrić J.  D-Optimal Design in the Development of Rheologically Improved In Situ Forming Ophthalmic Gel.  J Pharm Sci  2018    PubMed ID: 29421217
4192  Beiβner N, Mattern K, Dietzel A, Reichl S.  DynaMiTES - A dynamic cell culture platform for in vitro drug testing PART 2 - Ocular DynaMiTES for drug absorption studies of the anterior eye.  Eur J Pharm Biopharm  2018    PubMed ID: 28377274
4193  Klinngam W, Fu R, Janga SR, Edman MC, Hamm-Alvarez SF.  Cathepsin S Alters the Expression of Pro-Inflammatory Cytokines and MMP-9, Partially through Protease-Activated Receptor-2, in Human Corneal Epithelial Cells.  Int J Mol Sci  2018    PubMed ID: 30423938
4477  Tan G, Li J, Yang Q, Wu A, Qu DY, Wang Y, Ye L, Bao J, Shao Y.  Air pollutant particulate matter 2.5 induces dry eye syndrome in mice.  Sci Rep  2018  8(1):17828  PubMed ID: 30546125
6350  Yamaguchi H, Takezawa T.  Fabrication of a Corneal Model Composed of Corneal Epithelial and Endothelial Cells via a Collagen Vitrigel Membrane Functioned as an Acellular Stroma and Its Application to the Corneal Permeability Test of Chemicals.  Drug Metab Dispos  2018  46(11):1684-1691  PubMed ID: 29844140
6351  Shi L, Stachon T, Seitz B, Wagenpfeil S, Langenbucher A, Szentmáry N.  The Effect of Antiamoebic Agents on Viability, Proliferation and Migration of Human Epithelial Cells, Keratocytes and Endothelial Cells, In Vitro.  Curr Eye Res.  2018  43(6):725-733  PubMed ID: 29528760
6352  Hou A, Tong L.  Expression, Regulation, and Effects of Interleukin-17f in the Human Ocular Surface.  Ocul Immunol Inflamm  2018  26(7):1069-1077  PubMed ID: 28537453
9462  Vidyarani Mohankumar, Sangeetha Ramalingam, Gowri Priya Chidambaranathan, Lalitha Prajna  Autophagy Induced by Type III Secretion System Toxins Enhances Clearance of Pseudomonas Aeruginosa From Human Corneal Epithelial Cells  Biochem Biophys Res Commun . 2018 Sep 10;503(3):1510-1515  2018  503(3):1510-1515  PubMed ID: 30031608
9755  Wu MF, Stachon T, Langenbucher A, Seitz B, Szentmáry N.  Effect of Amniotic Membrane Suspension (AMS) and Amniotic Membrane Homogenate (AMH) on Human Corneal Epithelial Cell Viability, Migration and Proliferation In Vitro.  Curr. Eye Res.  2017  42:351-357  PubMed ID: 27420558
9821  Kim HJ, Kim JY, Lee BH, Choi SH, Rhim H, Kim HC, Ahn SY, Jeong SW, Jang M, Cho IH, Nah SY.  Gintonin, an exogenous ginseng-derived LPA receptor ligand, promotes corneal wound healing.  J. Vet. Sci.  2017  18:387-397  PubMed ID: 27586470
9974  Santander-Borrego M, Taran E, Shadforth AM, Whittaker AK, Chirila TV, Blakey I.  Hydrogels with Lotus Leaf Topography: Investigating Surface Properties and Cell Adhesion.  Langmuir  2017  33:485-493  PubMed ID: 28054787
10189  Juretić M, Jurišić Dukovski B, Krtalić I, Reichl S, Cetina-Čižmek B, Filipović-Grčić J, Lovrić J, Pepić I.  HCE-T cell-based permeability model: A well-maintained or a highly variable barrier phenotype?  Eur J Pharm Sci  2017  104:23-30  PubMed ID: 28315467
10251  Nishida T, Kuse Y, Mochizuki K, Shimazawa M, Yamamoto T, Hara H.  Protective effects of fluoroquinolones on UV-induced damage of cultured ocular cell lines.  Eur. J. Pharmacol.  2017  806:59-66  PubMed ID: 28395990
10412  Hou A, Tin MQ, Tong L.  Toll-like receptor 2-mediated NF-kappa B pathway activation in ocular surface epithelial cells.  Eye Vis (Lond)  2017  4:17  PubMed ID: 28706958
10983  Meng Z, Klinngam W, Edman MC, Hamm-Alvarez SF.  Interferon-γ treatment in vitro elicits some of the changes in cathepsin S and antigen presentation characteristic of lacrimal glands and corneas from the NOD mouse model of Sjo¨gren’s Syndrome  PLoS One  2017  12:e0184781  PubMed ID: 28902875
6348  Rat P, Olivier E, Tanter C, Wakx A, Dutot M.  A fast and reproducible cell- and 96-well plate-based method for the evaluation of P2X7 receptor activation using YO-PRO-1 fluorescent dye.  J Biol Methods  2017  4(1):e64  PubMed ID: 31453224
6353  Faber K, Zorzi GK, Brazil NT, Rott MB, Teixeira HF.  siRNA-loaded liposomes: Inhibition of encystment of Acanthamoeba and toxicity on the eye surface.  Chem Biol Drug Des  2017  90(3):406-416  PubMed ID: 28135787
4818  Chirila TV, Suzuki S1, Papolla C.  A comparative investigation of Bombyx mori silk fibroin hydrogels generated by chemical and enzymatic cross-linking.  Biotechnol Appl Biochem  2017  64(6):771-781  PubMed ID: 28220960
9715  Esaki Y, Shimazaki A, Pellinen P.  Ocular Tolerability of Preservative-Free Tafluprost and Latanoprost: in vitro and in vivo Comparative Study.  Open Ophthalmol J  2016  10:146-53  PubMed ID: 27347250
9744  Hakkarainen JJ, Reinisalo M, Ragauskas S, Seppänen A, Kaja S, Kalesnykas G.  Acute cytotoxic effects of marketed ophthalmic formulations on human corneal epithelial cells.  Int J Pharm  2016  511:73-78  PubMed ID: 27374205
9749  Fuwa M, Ueda K, Akaishi T, Yamashita N, Kirihara T, Shimazaki A, Mano H, Kawazu K.  Advantages of Efficacy and Safety of Fixed-Dose Tafluprost/Timolol Combination Over Fixed-Dose Latanoprost/Timolol Combination.  PLoS ONE  2016  11:e0158797  PubMed ID: 27383260
9778  Koyama S, Narita E, Shimizu Y, Suzuki Y, Shiina T, Taki M, Shinohara N, Miyakoshi J.  Effects of Long-Term Exposure to 60 GHz Millimeter-Wavelength Radiation on the Genotoxicity and Heat Shock Protein (Hsp) Expression of Cells Derived from Human Eye.  Int J Environ Res Public Health  2016  13:  PubMed ID: 27509516
9779  Koyama S, Narita E, Shimizu Y, Shiina T, Taki M, Shinohara N, Miyakoshi J.  Twenty Four-Hour Exposure to a 0.12 THz Electromagnetic Field Does Not Affect the Genotoxicity, Morphological Changes, or Expression of Heat Shock Protein in HCE-T Cells.  Int J Environ Res Public Health  2016  13:  PubMed ID: 27527204
9965  Utsunomiya T, Ishibazawa A, Nagaoka T, Hanada K, Yokota H, Ishii N, Yoshida A.  Transforming Growth Factor-β Signaling Cascade Induced by Mechanical Stimulation of Fluid Shear Stress in Cultured Corneal Epithelial Cells.  Invest. Ophthalmol. Vis. Sci.  2016  57:6382-6388  PubMed ID: 27898984
10034  Jamil B, Abbasi R, Abbasi S, Imran M, Khan SU, Ihsan A, Javed S, Bokhari H, Imran M.  Encapsulation of Cardamom Essential Oil in Chitosan Nano-composites: In-vitro Efficacy on Antibiotic-Resistant Bacterial Pathogens and Cytotoxicity Studies.  Front Microbiol  2016  7:1580  PubMed ID: 27757108
5426  Kadmiel M, Janoshazi A, Xu X, Cidlowski JA.  Glucocorticoid action in human corneal epithelial cells establishes roles for corticosteroids in wound healing and barrier function of the eye.  Exp Eye Res  2016  152:10-33  PubMed ID: 27600171
6347  Yamaguchi H, Kojima H, Takezawa T.  Predictive performance of the Vitrigel-eye irritancy test method using 118 chemicals.  J Appl Toxicol  2016  36(8):1025-37  PubMed ID: 26472347
6355  Kölln C, Reichl S  Expression of glutathione transferases in corneal cell lines, corneal tissues and a human cornea construct.  Int J Pharm  2016  506(1-2):371-81  PubMed ID: 27113863
6356  Kölln C, Reichl S.  Cytochrome P450 Activity in Ex Vivo Cornea Models and a Human Cornea Construct.  J Pharm Sci  2016  105(7):2204-12  PubMed ID: 27212636
7260  Wu MF, Stachon T, Wang J, Song X, Colanesi S, Seitz B, Wagenpfeil S, Langenbucher A, Szentmary N.  Effect of Keratocyte Supernatant on Epithelial Cell Migration and Proliferation After Corneal Crosslinking (CXL).  Curr. Eye Res.  2015  41:466-73  PubMed ID: 26236938
7396  Hafner A, Lovri? J, Romi? MD, Jureti? M, Pepi? I, Cetina-?i?mek B, Filipovi?-Gr?i? J.  Evaluation of cationic nanosystems with melatonin using an eye-related bioavailability prediction model.  Eur J Pharm Sci  2015  75:142-50  PubMed ID: 25869457
7500  Verstraelen J, Reichl S.  Upregulation of P-glycoprotein expression by ophthalmic drugs in different corneal in-vitro models.  J. Pharm. Pharmacol.  2015  67:605-15  PubMed ID: 25643948
6337  Xu M, McCanna DJ, Sivak JG.  Use of the viability reagent PrestoBlue in comparison with alamarBlue and MTT to assess the viability of human corneal epithelial cells.  J Pharmacol Toxicol Methods  2015  71:1-7  PubMed ID: 25464019
6330  Shibata Y, Tanaka Y, Tomita T, Taogoshi T, Kimura Y, Chikama T, Kihira K.  Evaluation of corneal damage caused by iodine preparations using human corneal epithelial cells.  Jpn J Ophthalmol  2014  58(6):522-7  PubMed ID: 25230910
6332  Wang W, Despanie J, Shi P, Edman-Woolcott MC, Lin YA, Cui H, Heur JM, Fini ME, Hamm-Alvarez SF, MacKay JA.  Lacritin-mediated regeneration of the corneal epithelia by protein polymer nanoparticles.  J Mater Chem B  2014  2(46):8131-8141  PubMed ID: 25530855
6357  Wen L, Zhu M, Madigan MC, You J, King NJ, Billson FA, McClellan K, Sutton G, Petsoglou C2  Immunomodulatory effects of bone marrow-derived mesenchymal stem cells on pro-inflammatory cytokine-stimulated human corneal epithelial cells.  PLoS One.  2014  9(7):e101841  PubMed ID: 25003339
6358  Sharma S, Gupta D, Mohanty S, Jassal M, Agrawal AK, Tandon R.  Surface-modified electrospun poly(epsilon-caprolactone) scaffold with improved optical transparency and bioactivity for damaged ocular surface reconstruction.  Invest Ophthalmol Vis Sci  2014  55(2):899-907  PubMed ID: 24425860
6359  Mahita Kadmiel, John A Cidlowski  Functional Characterization of the Glucocorticoid Receptor in the Cornea  Investigative Ophthalmology & Visual Science  2014  55:5504 
6360  Anitua E, Muruzabal F, De la Fuente M, Merayo-Lloves J, Orive G.  Effects of heat-treatment on plasma rich in growth factors-derived autologous eye drop.  Exp Eye Res  2014  119:27-34  PubMed ID: 24345372
6364  Wang N, Zimmerman K, Raab RW, McKown RL, Hutnik CM, Talla V, Tyler MF 4th, Lee JK, Laurie GW.  Lacritin rescues stressed epithelia via rapid forkhead box O3 (FOXO3)-associated autophagy that restores metabolism.  J Biol Chem.  2013.  288(25):18146-61  PubMed ID: 23640897
6323  Xu M, Sivak JG, McCanna DJ.  Comparison of the effects of ophthalmic solutions on human corneal epithelial cells using fluorescent dyes.  J Ocul Pharmacol Ther  2013  29(9):794-802  PubMed ID: 23905770
6324  Yamaguchi H, Kojima H, Takezawa T.  Vitrigel-eye irritancy test method using HCE-T cells.  Toxicol Sci  2013  135(2):347-55  PubMed ID: 23872712
6325  Rahimi M, Youn HY, McCanna DJ, Sivak JG, Mikkelsen SR.  Application of cyclic biamperometry to viability and cytotoxicity assessment in human corneal epithelial cells.  Anal Bioanal Chem  2013  405(14):4975-9  PubMed ID: 23443523
6327  Tong L, Png E, Aihua H, Yong SS, Yeo HL, Riau A, Mendoz E, Chaurasia SS, Lim CT, Yiu TW, Iismaa SE.  Molecular mechanism of transglutaminase-2 in corneal epithelial migration and adhesion.  Biochim Biophys Acta.  2013  1833(6):1304-15  PubMed ID: 23466867
6328  Hiroyoshi Kasai et al  Comparison of efficacy and safety evaluation of latanoprost formulations with and without benzalkonium chloride  Pharmacology & Pharmacy   2013  4:377-384 
6361  Verstraelen J, Reichl S.  Expression analysis of MDR1, BCRP and MRP3 transporter proteins in different in vitro and ex vivo cornea models for drug absorption studies.  Int J Pharm  2013  441(1-2):765-75  PubMed ID: 23069916
6362  Borrelli M, Reichl S, Feng Y, Schargus M, Schrader S, Geerling G.  In vitro characterization and ex vivo surgical evaluation of human hair keratin films in ocular surface reconstruction after sterilization processing.  J Mater Sci Mater Med  2013  24(1):221-30  PubMed ID: 23015265
6363  Hogerheyde TA, Stephenson SA, Harkin DG, Bray LJ, Madden PW, Woolf MI, Richardson NA.  Evaluation of Eph receptor and ephrin expression within the human cornea and limbus.  Exp Eye Res  2013  107:110-20  PubMed ID: 23247085
6365  Raju R, Jian B, Hooks JJ, Nagineni CN.  Transforming growth factor-β regulates the expression of anosmin (KAL-1) in human retinal pigment epithelial cells.  Cytokine  2013  61(3):724-7  PubMed ID: 23357298
6333  George KA, Shadforth AM, Chirila TV, Laurent MJ, Stephenson SA, Edwards GA, Madden PW, Hutmacher DW, Harkin DG.  Effect of the sterilization method on the properties of Bombyx mori silk fibroin films.  Mater Sci Eng C Mater Biol Appl  2013  33(2):668-74  PubMed ID: 25427472
6366  Anitua E, Muruzabal F, Alcalde I, Merayo-Lloves J, Orive G.  Plasma rich in growth factors (PRGF-Endoret) stimulates corneal wound healing and reduces haze formation after PRK surgery.  Exp Eye Res  2013  115:153-61  PubMed ID: 23872360
6367  Paterson SM, Shadforth AM, Shaw JA, Brown DH, Chirila TV, Baker MV.  Improving the cellular invasion into PHEMA sponges by incorporation of the RGD peptide ligand: the use of copolymerization as a means to functionalize PHEMA sponges.  Mater Sci Eng C Mater Biol Appl  2013  33(8):4917-22  PubMed ID: 24094205
6368  Engelke M, Zorn-Kruppa M, Gabel D, Reisinger K, Rusche B, Mewes KR.  A human hemi-cornea model for eye irritation testing: quality control of production, reliability and predictive capacity.  Toxicol In Vitro  2013  27(1):458-68  PubMed ID: 22902307
6315  Liang H, Baudouin C, Daull P, Garrigue JS, Brignole-Baudouin F.  Ocular safety of cationic emulsion of cyclosporine in an in vitro corneal wound-healing model and an acute in vivo rabbit model.  Mol Vis  2012  18:2195-204  PubMed ID: 22919267
6369  Hattori M, Shimizu K, Katsumura K, Oku H, Sano Y, Matsumoto K, Yamaguchi Y, Ikeda T.  Effects of all-trans retinoic acid nanoparticles on corneal epithelial wound healing.  Graefes Arch Clin Exp Ophthalmol  2012  250(4):557-63  PubMed ID: 22048243
6370  Kinoshita S, Kawasaki S, Kitazawa K, Shinomiya K.  Establishment of a human conjunctival epithelial cell line lacking the functional TACSTD2 gene (an American Ophthalmological Society thesis).  Trans Am Ophthalmol Soc  2012  110:166-77  PubMed ID: 23818740
6371  Hahne M, Zorn-Kruppa M, Guzman G, Brandner JM, Haltner-Ukomado E, Wätzig H, Reichl S.  Prevalidation of a human cornea construct as an alternative to animal corneas for in vitro drug absorption studies.  J Pharm Sci  2012  101(8):2976-88  PubMed ID: 22581751
6372  Stefan M.Paterson et al  The synthesis and degradation of collagenase-degradable poly(2-hydroxyethyl methacrylate)-based hydrogels and sponges for potential applications as scaffolds in tissue engineering  Materials Science and Engineering: C   2012  32:2536-2544 
9093  Bernice Wright, Richard A Cave, Joseph P Cook, Vitaliy V Khutoryanskiy, Shengli Mi, Bo Chen, Martin Leyland, Che J Connon  Enhanced Viability of Corneal Epithelial Cells for Efficient Transport/Storage Using a Structurally Modified Calcium Alginate Hydrogel  Regen Med2  2012  7 (3), 295-307  PubMed ID: 22594324
7623  Sharma S, Mohanty S, Gupta D, Jassal M, Agrawal AK, Tandon R.  Cellular response of limbal epithelial cells on electrospun poly-ε-caprolactone nanofibrous scaffolds for ocular surface bioengineering: a preliminary in vitro study.  Mol. Vis.  2011  17:2898-910  PubMed ID: 22128237
7815  Kato M, Hagiwara Y, Oda T, Imamura-Takai M, Aono H, Nakamura M.  Beneficial pharmacological effects of selective glucocorticoid receptor agonist in external eye diseases.  J Ocul Pharmacol Ther  2011  27:353-60  PubMed ID: 21574866
7894  Png E, Lan W, Lazaroo M, Chen S, Zhou L, Tong L.  A new method of high-speed cellular protein separation and insight into subcellular compartmentalization of proteins.  Anal Bioanal Chem  2011  400:767-75  PubMed ID: 21380751
7974  Youn HY, McCanna DJ, Sivak JG, Jones LW.  In vitro ultraviolet-induced damage in human corneal, lens, and retinal pigment epithelial cells.  Mol. Vis.  2011  17:237-46  PubMed ID: 21270970
8152  Png E, Samivelu GK, Yeo SH, Chew J, Chaurasia SS, Tong L.  Hyperosmolarity-mediated mitochondrial dysfunction requires Transglutaminase-2 in human corneal epithelial cells.  J. Cell. Physiol.  2011  226:693-9  PubMed ID: 20717931
6311  Takezawa T, Nishikawa K, Wang PC.  Development of a human corneal epithelium model utilizing a collagen vitrigel membrane and the changes of its barrier function induced by exposing eye irritant chemicals.  Toxicol In Vitro  2011  25(6):1237-41  PubMed ID: 21641988
8041  Shah A, Farooq AV, Tiwari V, Kim MJ, Shukla D.  HSV-1 infection of human corneal epithelial cells: receptor-mediated entry and trends of re-infection.  Mol. Vis.  2010  16:2476-86  PubMed ID: 21139972
6374  Yamada T, Ueda T, Ugawa S, Ishida Y, Imayasu M, Koyama S, Shimada S.  Functional expression of transient receptor potential vanilloid 3 (TRPV3) in corneal epithelial cells: involvement in thermosensation and wound healing.  Exp Eye Res  2010  90(1):121-9  PubMed ID: 19793539
9081  Yuki Terasaka, Dai Miyazaki, Keiko Yakura, Tomoko Haruki, Yoshitsugu Inoue  Induction of IL-6 in Transcriptional Networks in Corneal Epithelial Cells After Herpes Simplex Virus Type 1 Infection  Invest Ophthalmol Vis Sci  2010  51 (5), 2441-9  PubMed ID: 20019363
8445  Dutot M, Liang H, Martin C, Rousseau D, Grynberg A, Warnet JM, Rat P.  Per os administered refined olive oil and marine PUFA-rich oils reach the cornea: possible role on oxidative stress through caveolin-1 modulation.  Nutr Metab (Lond)  2009  6:48  PubMed ID: 19930652
8502  Pauloin T, Dutot M, Liang H, Chavinier E, Warnet JM, Rat P.  Corneal protection with high-molecular-weight hyaluronan against in vitro and in vivo sodium lauryl sulfate-induced toxic effects.  Cornea  2009  28:1032-41  PubMed ID: 19724206
8624  Pauloin T, Dutot M, Joly F, Warnet JM, Rat P.  High molecular weight hyaluronan decreases UVB-induced apoptosis and inflammation in human epithelial corneal cells.  Mol. Vis.  2009  15:577-83  PubMed ID: 19325714
6376  Karla PK, Quinn TL, Herndon BL, Thomas P, Pal D, Mitra A.  Expression of multidrug resistance associated protein 5 (MRP5) on cornea and its role in drug efflux.  J Ocul Pharmacol Ther  2009  25(2):121-32  PubMed ID: 19323627
9077  Kozaburo Hayashi, Laura C Hooper, Barbara Detrick, John J Hooks  HSV Immune Complex (HSV-IgG: IC) and HSV-DNA Elicit the Production of Angiogenic Factor VEGF and MMP-9  Arch Virol  2009  154 (2), 219-26  PubMed ID: 19115032
8697  Liu L, Tiffany J, Dang Z, Dart JK, Watson SL, Daniels JT, Geerling G.  Nourish and nurture: development of a nutrient ocular lubricant.  Invest. Ophthalmol. Vis. Sci.  2008  50:2932-9  PubMed ID: 19074805
8755  Dutot M, Liang H, Pauloin T, Brignole-Baudouin F, Baudouin C, Warnet JM, Rat P.  Effects of toxic cellular stresses and divalent cations on the human P2X7 cell death receptor.  Mol. Vis.  2008  14:889-97  PubMed ID: 18490962
8777  Alison C. Dunn A Jessica A. Cobb A Ana N. Kantzios A Sung Jin Lee AMalisa Sarntinoranont A Roger Tran-Son-Tay A W. Gregory Sawyer  Friction Coefficient Measurement of Hydrogel Materialson Living Epithelial Cells  Tribology Letters  2008  30:13-19 
6299  Pauloin T, Dutot M, Warnet JM, Rat P.  In vitro modulation of preservative toxicity: high molecular weight hyaluronan decreases apoptosis and oxidative stress induced by benzalkonium chloride.  Eur J Pharm Sci.   2008  34(4-5):263-73  PubMed ID: 18534829
6377  Becker U, Ehrhardt C, Schneider M, Muys L, Gross D, Eschmann K, Schaefer UF, Lehr CM.  A comparative evaluation of corneal epithelial cell cultures for assessing ocular permeability.  Altern Lab Anim  2008  36(1):33-44  PubMed ID: 18333713
6378  Secker GA, Shortt AJ, Sampson E, Schwarz QP, Schultz GS, Daniels JT.  TGFbeta stimulated re-epithelialisation is regulated by CTGF and Ras/MEK/ERK signalling.  Exp Cell Res  2008  314(1):131-42  PubMed ID: 17915216
6379  Nishikiori N, Sawada N, Ohguro H.  Prevention of murine experimental corneal trauma by epigenetic events regulating claudin 6 and claudin 9.  Jpn J Ophthalmol  2008  52(3):195-203  PubMed ID: 18661270
6380  Benko S, Tozser J, Miklossy G, Varga A, Kadas J, Csutak A, Berta A, Rajnavolgyi E.  Constitutive and UV-B modulated transcription of Nod-like receptors and their functional partners in human corneal epithelial cells.  Mol Vis  2008  14:1575-83  PubMed ID: 18769647
8783  Cobb JA, Dunn AC, Kwon J, Sarntinoranont M, Sawyer WG, Tran-Son-Tay R.  A novel method for low load friction testing on living cells.  Biotechnol. Lett.  2007  30:801-6  PubMed ID: 18071637
6292  Miles DH, Thakur A, Cole N, Willcox MD.  The induction and suppression of the apoptotic response of HSV-1 in human corneal epithelial cells.  Invest Ophthalmol Vis Sci  2007  48(2):789-96  PubMed ID: 17251479
6381  Ho JH, Chuang CH, Ho CY, Shih YR, Lee OK, Su Y.  Internalization is essential for the antiapoptotic effects of exogenous thymosin beta-4 on human corneal epithelial cells.  Invest Ophthalmol Vis Sci  2007  48(1):27-33  PubMed ID: 17197512
8874  Liu L, Hartwig D, Harloff S, Herminghaus P, Wedel T, Kasper K, Geerling G.  Corneal epitheliotrophic capacity of three different blood-derived preparations.  Invest. Ophthalmol. Vis. Sci.  2006  47:2438-44  PubMed ID: 16723454
9066  Liu L, Hartwig D, Harloff S, Herminghaus P, Wedel T, Geerling G.  An optimised protocol for the production of autologous serum eyedrops.  Graefes Arch. Clin. Exp. Ophthalmol.  2005  243:706-14  PubMed ID: 15756576
9075  Joo JH, Alpatov R, Munguba GC, Jackson MR, Hunt ME, Sugrue SP.  Reduction of Pnn by RNAi induces loss of cell-cell adhesion between human corneal epithelial cells.  Mol. Vis.  2005  11:133-42  PubMed ID: 15735603
6382  Gabison EE1, Mourah S, Steinfels E, Yan L, Hoang-Xuan T, Watsky MA, De Wever B, Calvo F, Mauviel A, Menashi S.  Differential expression of extracellular matrix metalloproteinase inducer (CD147) in normal and ulcerated corneas: role in epithelio-stromal interactions and matrix metalloproteinase induction.  Am J Pathol  2005  166(1):209-19  PubMed ID: 15632013
9344  Zimowska G, Shi J, Munguba G, Jackson MR, Alpatov R, Simmons MN, Shi Y, Sugrue SP.  Pinin/DRS/memA interacts with SRp75, SRm300 and SRrp130 in corneal epithelial cells.  Invest. Ophthalmol. Vis. Sci.  2003  44:4715-23  PubMed ID: 14578391
6274  Miles D, Athmanathan S, Thakur A, Willcox M.  A novel apoptotic interaction between HSV-1 and human corneal epithelial cells.  Curr Eye Res  2003  26(3-4):165-74  PubMed ID: 12815544
6383  Papa V, Leonardi A, Getuli C, Pacelli V, Russo P, Milazzo G.  Effect of ofloxacin and netilmicin on human corneal and conjunctival cells in vitro.  J Ocul Pharmacol Ther  2003  19(6):535-45  PubMed ID: 14733711

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