Cell No. : Cell Name
RCB2279 : WA-hT
update : 2024/08/14
|
| Comment | Human cell line derived from lung cancer. Small cell carcinoma. Mouse WA-mFib cells are the stromal cells for this cell line. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Takiguchi, Yuichi
|
| Originator |
Takiguchi, Yuichi
|
| Year of deposit |
2004
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
56 years
|
| Tissue |
lung
|
| Disease name |
small cell lung carcinoma
|
| Metastatic ability |
Yes
|
| Metastatic tissue |
lymph node
|
| Classification |
cancer
|
| Year of origin |
1990
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_8766
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
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(-)
|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
11
|
| Reference |
|
267
Hironori Ashinuma, Yuichi Takiguchi, Satoru Kitazono, Miyako Kitazono-Saitoh, Atsushi Kitamura, Yuji Tada, Katsushi Kurosu, Emiko Sakaida, Ikuo Sekine, Nobuhiro Tanabe, Koichiro Tatsumi
Abstract 2737: Anti-proliferative action of metformin on various types of human lung cancer cell lines
Cancer Research
2012
72:2737-2737
DOI: 10.1158/1538-7445.am2012-2737
|
|
3487
Sugimoto T, Takiguchi Y, Kurosu K, Kasahara Y, Tanabe N, Tatsumi K, Hiroshima K, Minamihisamatsu M, Miyamoto T, Kuriyama T.
Growth factor-mediated interaction between tumor cells and stromal fibroblasts in an experimental model of human small-cell lung cancer.
Oncol Rep
2005
14(4):823-30
PubMed ID: 16142338
|
| User's Publication |
|
22524
Kasai F, Fukushima M, Miyagi Y, Nakamura Y.
Genetic diversity among the present Japanese population: evidence from genotyping of human cell lines established in Japan
Hum Cell
2024
37(4):944-950
PubMed ID: 38639832
DOI: 10.1007/s13577-024-01055-0
|
|
21380
Tagawa Y, Sakagami H, Tanuma SI, Amano S, Uota S, Bandow K, Tomomura M, Uesawa Y, Takao K, Sugita Y, Yamamoto N, Sakashita H, Nakakaji R, Koizumi T, Mitsudo K, Tohnai I.
Potentiation of Anticancer Activity of G2/M Blockers by Mild Hyperthermia.
Anticancer Res
2023
43(8):3429-3439
PubMed ID: 37500171
DOI: 10.21873/anticanres.16518
|
|
20884
Shindo A, Kusano M, Sakagami H, Amano S, Inomata M, Abe M, Okazawa M, Ooka T.
Comparison of UVC Sensitivity and Dectin-2 Expression Between Malignant and Non-malignant Cells.
In Vivo
2022
36(5):2116-2125
PubMed ID: 36099100
DOI: 10.21873/invivo.12937
|
|
15500
Zhu LM, Shi HX, Sugimoto M, Bandow K, Sakagami H, Amano S, Deng HB, Ye QY, Gai Y, Xin XL, Xu ZY.
Feiyanning Formula Induces Apoptosis of Lung Adenocarcinoma Cells by Activating the Mitochondrial Pathway
Front Oncol
2021
11:690878
PubMed ID: 34277435
DOI: 10.3389/fonc.2021.690878
|
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13784
Nii T, Kuwahara T, Makino K, Tabata Y.
A Co-Culture System of Three-Dimensional Tumor-Associated Macrophages and Three-Dimensional Cancer-Associated Fibroblasts Combined with Biomolecule Release for Cancer Cell Migration
Tissue Eng Part A
2020
26(23-24):1272-1282.
PubMed ID: 32434426
DOI: 10.1089/ten.TEA.2020.0095
|
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14060
Nii T, Makino K, Tabata Y.
A cancer invasion model of cancer-associated fibroblasts aggregates combined with TGF-β1 release system
Regen Ther
2020
14:196-204
PubMed ID: 32154334
DOI: 10.1016/j.reth.2020.02.003
|
|
12301
Teruki Nii, Kimiko Makino, Yasuhiko Tabata
A Cancer Invasion Model Combined with Cancer-Associated Fibroblasts Aggregates Incorporating Gelatin Hydro
Tissue Eng Part C Methods
2019
25(12):711-720
PubMed ID: 31621504
DOI: 10.1089/ten.TEC.2019.0189
|
|
9581
Shimobaba S, Taga S, Akizuki R, Hichino A, Endo S, Matsunaga T, Watanabe R, Yamaguchi M, Yamazaki Y, Sugatani J, Ikari A.
Claudin-18 inhibits cell proliferation and motility mediated by inhibition of phosphorylation of PDK1 and Akt in human lung adenocarcinoma A549 cells.
Biochim. Biophys. Acta
2016
1863:1170-8
PubMed ID: 26919807
DOI: 10.1016/j.bbamcr.2016.02.015
|
|
9731
Mizutani N, Abe M, Matsuoka S, Kajino K, Wakiya M, Ohtsuji N, Hatano R, Morimoto C, Hino O.
Establishment of anti-mesothelioma monoclonal antibodies.
BMC Res Notes
2016
9:324
PubMed ID: 27342200
DOI: 10.1186/s13104-016-2128-x
|
|
12718
Ikari A, Sato T, Watanabe R, Yamazaki Y, Sugatani J.
Increase in claudin-2 expression by an EGFR/MEK/ERK/c-Fos pathway in lung adenocarcinoma A549 cells
Biochim Biophys Acta
2012
1823(6):1110-8
PubMed ID: 22546605
DOI: 10.1016/j.bbamcr.2012.04.005
|
|
6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
DOI: 10.1111/j.1749-0774.2009.00071.x
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