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Cell No. : Cell Name
RCB2279 : WA-hT  update : 2023/01/04
CommentHuman cell line derived from lung cancer. Small cell carcinoma. Mouse WA-mFib cells are the stromal cells for this cell line.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takiguchi, Yuichi
Originator Takiguchi, Yuichi
Year of deposit 2004
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 56 years
Tissue lung
Disease name small cell lung carcinoma
Metastastatic ability Yes
Metastatsd tissue lymph node
Classification cancer
Year of origin 1990
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8766
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 9

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3487  Sugimoto T, Takiguchi Y, Kurosu K, Kasahara Y, Tanabe N, Tatsumi K, Hiroshima K, Minamihisamatsu M, Miyamoto T, Kuriyama T.  Growth factor-mediated interaction between tumor cells and stromal fibroblasts in an experimental model of human small-cell lung cancer.  Oncol Rep  2005  14(4):823-30  PubMed ID: 16142338  

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User's Publication
20884  Shindo A, Kusano M, Sakagami H, Amano S, Inomata M, Abe M, Okazawa M, Ooka T.  Comparison of UVC Sensitivity and Dectin-2 Expression Between Malignant and Non-malignant Cells.  In Vivo  2022  36(5):2116-2125  PubMed ID: 36099100   DOI: 10.21873/invivo.12937
15500  Zhu LM, Shi HX, Sugimoto M, Bandow K, Sakagami H, Amano S, Deng HB, Ye QY, Gai Y, Xin XL, Xu ZY.  Feiyanning Formula Induces Apoptosis of Lung Adenocarcinoma Cells by Activating the Mitochondrial Pathway  Front Oncol   2021  11:690878  PubMed ID: 34277435   DOI: 10.3389/fonc.2021.690878
13784  Nii T, Kuwahara T, Makino K, Tabata Y.  A Co-Culture System of Three-Dimensional Tumor-Associated Macrophages and Three-Dimensional Cancer-Associated Fibroblasts Combined with Biomolecule Release for Cancer Cell Migration  Tissue Eng Part A  2020  26(23-24):1272-1282.  PubMed ID: 32434426   DOI: 10.1089/ten.TEA.2020.0095
14060  Nii T, Makino K, Tabata Y.  A cancer invasion model of cancer-associated fibroblasts aggregates combined with TGF-β1 release system  Regen Ther  2020  14:196-204  PubMed ID: 32154334   DOI: 10.1016/j.reth.2020.02.003
12301  Teruki Nii, Kimiko Makino, Yasuhiko Tabata  A Cancer Invasion Model Combined with Cancer-Associated Fibroblasts Aggregates Incorporating Gelatin Hydro  Tissue Eng Part C Methods  2019  25(12):711-720  PubMed ID: 31621504   DOI: 10.1089/ten.TEC.2019.0189
9581  Shimobaba S, Taga S, Akizuki R, Hichino A, Endo S, Matsunaga T, Watanabe R, Yamaguchi M, Yamazaki Y, Sugatani J, Ikari A.  Claudin-18 inhibits cell proliferation and motility mediated by inhibition of phosphorylation of PDK1 and Akt in human lung adenocarcinoma A549 cells.  Biochim. Biophys. Acta  2016  1863:1170-8  PubMed ID: 26919807   DOI: 10.1016/j.bbamcr.2016.02.015
9731  Mizutani N, Abe M, Matsuoka S, Kajino K, Wakiya M, Ohtsuji N, Hatano R, Morimoto C, Hino O.  Establishment of anti-mesothelioma monoclonal antibodies.  BMC Res Notes  2016  9:324  PubMed ID: 27342200   DOI: 10.1186/s13104-016-2128-x
12718  Ikari A, Sato T, Watanabe R, Yamazaki Y, Sugatani J.  Increase in claudin-2 expression by an EGFR/MEK/ERK/c-Fos pathway in lung adenocarcinoma A549 cells  Biochim Biophys Acta  2012  1823(6):1110-8  PubMed ID: 22546605   DOI: 10.1016/j.bbamcr.2012.04.005
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x

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