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Cell No. : Cell Name
RCB2278 : GSU  update : 2023/10/16
CommentHuman cell line derived from gastric cancer. Established from the cells in ascites.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hato, Tsuneaki
Originator Hato, Tsuneaki
Year of deposit 2004
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 37 years
Tissue stomach, ascites meta
Disease name gastric cancer
Metastatic ability Yes
Metastatic tissue lymph node, ascites meta
Classification cancer
Year of origin 1994
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_8877
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 0
User's Publication 14

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User's Publication
21415  Fukuoka S, Koga Y, Yamauchi M, Koganemaru S, Yasunaga M, Shitara K, Doi T, Yoshino T, Kuronita T, Elenbaas B, Wahra P, Zhang H, Crowley L, Jenkins MH, Clark A, Kojima T.  p70S6K/Akt dual inhibitor DIACC3010 is efficacious in preclinical models of gastric cancer alone and in combination with trastuzumab.  Sci Rep  2023  13(1):16017  PubMed ID: 37749105   DOI: 10.1038/s41598-023-40612-9
21448  Ohta A, Tanada M, Shinohara S, Morita Y, Nakano K, Yamagishi Y, Takano R, Kariyuki S, Iida T, Matsuo A, Ozeki K, Emura T, Sakurai Y, Takano K, Higashida A, Kojima M, Muraoka T, Takeyama R, Kato T, Kimura K, Ogawa K, Ohara K, Tanaka S, Kikuchi Y, Hisada N, Hayashi R, Nishimura Y, Nomura K, Tachibana T, Irie M, Kawada H, Torizawa T, Murao N, Kotake T, Tanaka M, Ishikawa S, Miyake T, Tamiya M, Arai M, Chiyoda A, Akai S, Sase H, Kuramoto S, Ito T, Shiraishi T, Kojima T, Iikura H.  Validation of a New Methodology to Create Oral Drugs beyond the Rule of 5 for Intracellular Tough Targets.  J Am Chem Soc  2023    PubMed ID: 37874670   DOI: 10.1021/jacs.3c07145
20609  Shen Y, Eng JS, Fajardo F, Liang L, Li C, Collins P, Tedesco D, Nolan-Stevaux O.  Cancer cell-intrinsic resistance to BiTE therapy is mediated by loss of CD58 costimulation and modulation of the extrinsic apoptotic pathway.  J Immunother Cancer  2022  10(3)  PubMed ID: 35296559   DOI: 10.1136/jitc-2021-004348
20664  Han Y, Dong Q, Liu T, Chen X, Yu C, Zhang Y.  The novel mechanism of Med12-mediated drug resistance in a TGFBR2-independent manner.  Biochem Biophys Res Commun  2022  610:1-7  PubMed ID: 35461070   DOI: 10.1016/j.bbrc.2022.04.029
12041  Hirano T, Shinsato Y, Tanabe K, Higa N, Kamil M, Kawahara K, Yamamoto M, Minami K, Shimokawa M, Arigami T, Yanagita S, Matushita D, Uenosono Y, Ishigami S, Kijima Y, Maemura K, Kitazono I, Tanimoto A, Furukawa T, Natsugoe S.  FARP1 boosts CDC42 activity from integrin αvβ5 signaling and correlates with poor prognosis of advanced gastric cancer.  Oncogenesis  2020    PubMed ID: 32029704   DOI: 10.1038/s41389-020-0190-7
12127  An L, Nie P, Chen M, Tang Y, Zhang H, Guan J, Cao Z, Hou C, Wang W, Zhao Y, Xu H, Jiao S, Zhou Z.  MST4 kinase suppresses gastric tumorigenesis by limiting YAP activation via a non-canonical pathway.  J. Exp. Med.  2020    PubMed ID: 32271880   DOI: 10.1084/jem.20191817
13385  Neggers JE, Paolella BR, Asfaw A, Rothberg MV, Skipper TA, Yang A, Kalekar RL, Krill-Burger JM, Dharia NV, Kugener G, Kalfon J, Yuan C, Dumont N, Gonzalez A, Abdusamad M, Li YY, Spurr LF, Wu WW, Durbin AD, Wolpin BM, Piccioni F, Root DE, Boehm JS, Cherniack AD, Tsherniak A, Hong AL, Hahn WC, Stegmaier K, Golub TR, Vazquez F, Aguirre AJ.  Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q.  Cell Rep  2020    PubMed ID: 33326793   DOI: 10.1016/j.celrep.2020.108493
14877  Liu T, Han Y, Yu C, Ji Y, Wang C, Chen X, Wang X, Shen J, Zhang Y, Lang JY.  MYC predetermines the sensitivity of gastrointestinal cancer to antifolate drugs through regulating TYMS transcription  EBioMedicine  2019  48:289-300  PubMed ID: 31648989   DOI: 10.1016/j.ebiom.2019.10.003
10662  Miyamoto S, Nagamura Y, Nakabo A, Okabe A, Yanagihara K, Fukami K, Sakai R, Yamaguchi H.  Aberrant alternative splicing of RHOA is associated with loss of its expression and activity in diffuse-type gastric carcinoma cells.  Biochem. Biophys. Res. Commun.  2018  495:1942-1947  PubMed ID: 29247652   DOI: 10.1016/j.bbrc.2017.12.067
9978  Kneissl J, Hartmann A, Pfarr N, Erlmeier F, Lorber T, Keller S, Zwingenberger G, Weichert W, Luber B.  Influence of the HER receptor ligand system on sensitivity to cetuximab and trastuzumab in gastric cancer cell lines.  J. Cancer Res. Clin. Oncol.  2017  143:573-600  PubMed ID: 27933395   DOI: 10.1007/s00432-016-2308-z
14615  Nambara S, Masuda T, Nishio M, Kuramitsu S, Tobo T, Ogawa Y, Hu Q, Iguchi T, Kuroda Y, Ito S, Eguchi H, Sugimachi K, Saeki H, Oki E, Maehara Y, Suzuki A, Mimori K.  Antitumor effects of the antiparasitic agent ivermectin via inhibition of Yes-associated protein 1 expression in gastric cancer  Oncotarget   2017  8(64):107666-107677  PubMed ID: 29296196   DOI: 10.18632/oncotarget.22587
5812  Hirashita Y et al  Reduced phosphorylation of ribosomal protein S6 is associated with sensitivity to MEK inhibition in gastric cancer cells.  Cancer Sci  2016  107(12):1919-1928  PubMed ID: 27699948   DOI: 10.1111/cas.13094
15081  Awazu Y, Nakamura K, Mizutani A, Kakoi Y, Iwata H, Yamasaki S, Miyamoto N, Imamura S, Miki H, Hori A.  A novel inhibitor of c-Met and VEGF receptor tyrosine kinases with a broad spectrum of in vivo antitumor activities  Mol Cancer Ther  2013  12(6):913-24  PubMed ID: 23548264   DOI: 10.1158/1535-7163.MCT-12-1011
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x

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