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Cell No. : Cell Name
RCB2277 : GSS  update : 2021/12/21
CommentHuman gastric cancer cell line derived from metastasis at liver.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hato, Tsuneaki
Originator Hato, Tsuneaki
Year of deposit 2004
Original cell 293
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 75 years
Tissue stomach, liver meta
Disease name gastric cancer
Metastatic ability Yes
Metastatic tissue lymph node, liver
Classification cancer
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_8876
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 0
User's Publication 7


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Reference

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User's Publication
12127  An L, Nie P, Chen M, Tang Y, Zhang H, Guan J, Cao Z, Hou C, Wang W, Zhao Y, Xu H, Jiao S, Zhou Z.  MST4 kinase suppresses gastric tumorigenesis by limiting YAP activation via a non-canonical pathway.  J. Exp. Med.  2020    PubMed ID: 32271880
14804  Xing M, Ooi WF, Tan J, Qamra A, Lee PH, Li Z, Xu C, Padmanabhan N, Lim JQ, Guo YA, Yao X, Amit M, Ng LM, Sheng T, Wang J, Huang KK, Anene-Nzelu CG, Ho SWT, Ray M, Ma L, Fazzi G, Lim KJ, Wijaya GC, Zhang S, Nandi T, Yan T, Chang MM, Das K, Isa ZFA, Wu J, Poon PSY, Lam YN, Lin JS, Tay ST, Lee MH, Tan ALK, Ong X, White K, Rozen SG, Beer M, Foo RSY, Grabsch HI, Skanderup AJ, Li S, Teh BT, Tan P.  Genomic and epigenomic EBF1 alterations modulate TERT expression in gastric cancer  J Clin Invest  2020  130(6):3005-3020  PubMed ID: 32364535
4189  Hashizume H, Iijima K, Yamashita K, Kimura T, Wada SI, Sawa R, Igarashi M.  Valgamicin C, a novel cyclic depsipeptide containing the unusual amino acid cleonine, and related valgamicins A, T and V produced by Amycolatopsis sp. ML1-hF4.  J. Antibiot.  2017    PubMed ID: 29138482
5812  Hirashita Y et al  Reduced phosphorylation of ribosomal protein S6 is associated with sensitivity to MEK inhibition in gastric cancer cells.  Cancer Sci  2016  107(12):1919-1928  PubMed ID: 27699948
15817  Endo F, Nishizuka SS, Kume K, Ishida K, Katagiri H, Ishida K, Sato K, Iwaya T, Koeda K, Wakabayashi G.  A compensatory role of NF-κB to p53 in response to 5-FU-based chemotherapy for gastric cancer cell lines  PLoS One  2014  9(2):e90155  PubMed ID: 24587255
6316  Miyaki Y, Suzuki K, Koizumi K, Kato T, Saito M, Kamiyama H, Maeda T, Shibata K, Shiya N, Konishi F.  Identification of a potent epigenetic biomarker for resistance to camptothecin and poor outcome to irinotecan-based chemotherapy in colon cancer.  Int J Oncol  2012  40(1):217-26  PubMed ID: 21901246
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309



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