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Cell No. : Cell Name
RCB2265 : HE49  update : 2021/05/20
CommentHuman fibroblast-like cells derived from embryo. Not immortalized.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Watanabe, Masami
Originator Watanabe, Masami
Year of deposit 2002
Animal human < Mammals
Genus Homo
Species sapiens
Tissue embryo
Classification normal
Year of origin 1989
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_0T98
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 1mM Sodium Pyruvate + 0.2mM Serine + 0.2mM Aspartate
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 11
User's Publication 0


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Reference
5490  Miyakoda M, Suzuki K, Kodama S, Watanabe M.  Activation of ATM and phosphorylation of p53 by heat shock.  Oncogene  2002  21(7):1090-6  PubMed ID: 11850826
5488  Suzuki K, Mori I, Nakayama Y, Miyakoda M, Kodama S, Watanabe M.  Radiation-induced senescence-like growth arrest requires TP53 function but not telomere shortening.  Radiat Res  2001  155(1 Pt 2):248-253  PubMed ID: 11121242
5489  Suzuki K, Kodama S, Watanabe M.  Extremely low-dose ionizing radiation causes activation of mitogen-activated protein kinase pathway and enhances proliferation of normal human diploid cells.  Cancer Res  2001  61(14):5396-401.  PubMed ID: 11454682
5482  Ghosh JC, Izumida Y, Suzuki K, Kodama S, Watanabe M.  Dose-dependent biphasic accumulation of TP53 protein in normal human embryo cells after X irradiation.  Radiat Res  2000  153(3):305-11  PubMed ID: 10669552
5481  Ghosh JC1, Suzuki K, Kodama S, Watanabe M.  Effects of protein kinase inhibitors on the accumulation kinetics of p53 protein in normal human embryo cells following X-irradiation.  J Radiat Res  1999  40(1):23-37  PubMed ID: 10408175
5486  Miyakoda M, Nakahata K, Suzuki K, Kodama S, Watanabe M.  Heat-induced G1 arrest is dependent on p53 function but not on RB dephosphorylation.  Biochem Biophys Res Commun  1999  266(2):377-81  PubMed ID: 10600511
5487  Suzuki K, Kodama S, Watanabe M.  Recruitment of ATM protein to double strand DNA irradiated with ionizing radiation.  J Biol Chem  1999  274(36):25571-5  PubMed ID: 10464290
3503  Yang Z, Kodama S, Suzuki K, Watanabe M.  Telomerase activity, telomere length, and chromosome aberrations in the extension of life span of human embryo cells induced by low-dose X-rays.  J Radiat Res  1998  39(1):35-51  PubMed ID: 9610031
5483  Suzuki K, Kodama S, Watanabe M.  Effect of low-dose preirradiation on induction of the HSP70B-LacZ fusion gene in human cells treated with heat shock.  Radiat Res  1998  149(2):195-201  PubMed ID: 9457900
5484  Suzuki K, Takahara R, Kodama S, Watanabe M.  In situ detection of chromosome bridge formation and delayed reproductive death in normal human embryonic cells surviving X irradiation.  Radiat Res  1998  150(4):375-81  PubMed ID: 9768850
5485  Suzuki K, Kodama S, Watanabe M.  Suppressive effect of low-dose preirradiation on genetic instability induced by X rays in normal human embryonic cells.  Radiat Resc  1998  150(6):656-62  PubMed ID: 9840185

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