Cell No. : Cell Name
RCB2265 : HE49
update : 2023/10/16
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| Comment | Human fibroblast-like cells derived from embryo. Not immortalized. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Watanabe, Masami
|
| Originator |
Watanabe, Masami
|
| Year of deposit |
2002
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Tissue |
embryo
|
| Classification |
normal
|
| Year of origin |
1989
|
|
Lifespan |
finite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_0T98
|
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM + 10% FBS + 1mM Sodium Pyruvate + 0.2mM Serine + 0.2mM Aspartate
|
| Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin
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Culture information
|
Passage ratio |
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1 : 4 split
|
| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
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37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
11
|
| User's Publication |
0
|
| Reference |
|
5490
Miyakoda M, Suzuki K, Kodama S, Watanabe M.
Activation of ATM and phosphorylation of p53 by heat shock.
Oncogene
2002
21(7):1090-6
PubMed ID: 11850826
DOI: 10.1038/sj.onc.1205196
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5488
Suzuki K, Mori I, Nakayama Y, Miyakoda M, Kodama S, Watanabe M.
Radiation-induced senescence-like growth arrest requires TP53 function but not telomere shortening.
Radiat Res
2001
155(1 Pt 2):248-253
PubMed ID: 11121242
DOI: 10.1667/0033-7587(2001)155[0248:rislga]2.0.co;2
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5489
Suzuki K, Kodama S, Watanabe M.
Extremely low-dose ionizing radiation causes activation of mitogen-activated protein kinase pathway and enhances proliferation of normal human diploid cells.
Cancer Res
2001
61(14):5396-401.
PubMed ID: 11454682
|
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5482
Ghosh JC, Izumida Y, Suzuki K, Kodama S, Watanabe M.
Dose-dependent biphasic accumulation of TP53 protein in normal human embryo cells after X irradiation.
Radiat Res
2000
153(3):305-11
PubMed ID: 10669552
DOI: 10.1667/0033-7587(2000)153[0305:ddbaot]2.0.co;2
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5481
Ghosh JC1, Suzuki K, Kodama S, Watanabe M.
Effects of protein kinase inhibitors on the accumulation kinetics of p53 protein in normal human embryo cells following X-irradiation.
J Radiat Res
1999
40(1):23-37
PubMed ID: 10408175
DOI: 10.1269/jrr.40.23
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5486
Miyakoda M, Nakahata K, Suzuki K, Kodama S, Watanabe M.
Heat-induced G1 arrest is dependent on p53 function but not on RB dephosphorylation.
Biochem Biophys Res Commun
1999
266(2):377-81
PubMed ID: 10600511
DOI: 10.1006/bbrc.1999.1829
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5487
Suzuki K, Kodama S, Watanabe M.
Recruitment of ATM protein to double strand DNA irradiated with ionizing radiation.
J Biol Chem
1999
274(36):25571-5
PubMed ID: 10464290
DOI: 10.1074/jbc.274.36.25571
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3503
Yang Z, Kodama S, Suzuki K, Watanabe M.
Telomerase activity, telomere length, and chromosome aberrations in the extension of life span of human embryo cells induced by low-dose X-rays.
J Radiat Res
1998
39(1):35-51
PubMed ID: 9610031
DOI: 10.1269/jrr.39.35
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5483
Suzuki K, Kodama S, Watanabe M.
Effect of low-dose preirradiation on induction of the HSP70B-LacZ fusion gene in human cells treated with heat shock.
Radiat Res
1998
149(2):195-201
PubMed ID: 9457900
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5484
Suzuki K, Takahara R, Kodama S, Watanabe M.
In situ detection of chromosome bridge formation and delayed reproductive death in normal human embryonic cells surviving X irradiation.
Radiat Res
1998
150(4):375-81
PubMed ID: 9768850
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5485
Suzuki K, Kodama S, Watanabe M.
Suppressive effect of low-dose preirradiation on genetic instability induced by X rays in normal human embryonic cells.
Radiat Resc
1998
150(6):656-62
PubMed ID: 9840185
|
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