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Cell No. : Cell Name
RCB2253 : HKb20  update : 2018/07/06
CommentSubline of HEK293 cell line, expressing BtR175b (Cry 1Aa receptor).
Comment from the depositor
Terms and conditionsNo restriction, do not use in a commercial purpose.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Sugimoto, Kenji
Originator Sugimoto, K.
Year of deposit 2002
Original cell 293
Animal human < Mammals
Genus Homo
Species sapiens
Tissue kidney
Classification transformed
Recombinant recombinant
Exogene pIRESneo2
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS + 800μg/ml G418
Antibiotics Free
Passage method 0.02% EDTA
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
3437  Tsuda Y, Nakatani F, Hashimoto K, Ikawa S, Matsuura C, Fukada T, Sugimoto K, Himeno M  Cytotoxic activity of Bacillus thuringiensis Cry proteins on mammalian cells transfected with cadherin-like Cry receptor gene of Bombyx mori (silkworm).  Biochem J  2003  369:697-703  PubMed ID: 12403648
3436  Ikawa S, Tsuda Y, Fukada T, Sugimoto K, Himeno M  cDNA cloning of the Cry1Aa receptor variants from Bombyx mori and their expression in mammalian cells.  Biosci Biotechnol Biochem  2000  64:2682-5  PubMed ID: 11210135

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