Cell No. : Cell Name
RCB2253 : HKb20
update : 2024/11/11
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| Comment | A subline of 293. Expressing BtR175b (Cry 1Aa receptor). |
| Comment from the depositor | |
| Terms and conditions | No restriction, do not use in a commercial purpose. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Sugimoto, Kenji
|
| Originator |
Sugimoto, K.
|
| Year of deposit |
2002
|
| Original cell |
293
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Tissue |
kidney
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
pIRESneo2
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_E065
|
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deposit info |
lot info |
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Medium |
Medium List |
|
Culture type |
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Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS + 800μg/ml G418
|
| Antibiotics |
|
Free
|
|
Passage method |
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0.02% EDTA
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Mycoplasma |
(-)
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|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
0
|
| Reference |
|
3437
Tsuda Y, Nakatani F, Hashimoto K, Ikawa S, Matsuura C, Fukada T, Sugimoto K, Himeno M
Cytotoxic activity of Bacillus thuringiensis Cry proteins on mammalian cells transfected with cadherin-like Cry receptor gene of Bombyx mori (silkworm).
Biochem J
2003
369:697-703
PubMed ID: 12403648
DOI: 10.1042/BJ20021401
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3436
Ikawa S, Tsuda Y, Fukada T, Sugimoto K, Himeno M
cDNA cloning of the Cry1Aa receptor variants from Bombyx mori and their expression in mammalian cells.
Biosci Biotechnol Biochem
2000
64:2682-5
PubMed ID: 11210135
DOI: 10.1271/bbb.64.2682
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