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Cell No. : Cell Name
RCB2253 : HKb20  update : 2021/05/20
CommentA subline of 293. Expressing BtR175b (Cry 1Aa receptor).
Comment from the depositor
Terms and conditionsNo restriction, do not use in a commercial purpose.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sugimoto, Kenji
Originator Sugimoto, K.
Year of deposit 2002
Original cell 293
Animal human < Mammals
Genus Homo
Species sapiens
Tissue kidney
Classification transformed
Recombinant recombinant
Exogene pIRESneo2
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_E065
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS + 800μg/ml G418
Antibiotics Free
Passage method 0.02% EDTA
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
3437  Tsuda Y, Nakatani F, Hashimoto K, Ikawa S, Matsuura C, Fukada T, Sugimoto K, Himeno M  Cytotoxic activity of Bacillus thuringiensis Cry proteins on mammalian cells transfected with cadherin-like Cry receptor gene of Bombyx mori (silkworm).  Biochem J  2003  369:697-703  PubMed ID: 12403648   DOI: 10.1042/BJ20021401
3436  Ikawa S, Tsuda Y, Fukada T, Sugimoto K, Himeno M  cDNA cloning of the Cry1Aa receptor variants from Bombyx mori and their expression in mammalian cells.  Biosci Biotechnol Biochem  2000  64:2682-5  PubMed ID: 11210135   DOI: 10.1271/bbb.64.2682

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