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Cell No. : Cell Name
RCB2239 : HE19  update : 2021/05/20
CommentHuman fibroblast-like cells derived from embryo. Not immortalized.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Watanabe, Masami
Originator Watanabe, Masami
Year of deposit 2002
Animal human < Mammals
Genus Homo
Species sapiens
Tissue embryo
Classification normal
Year of origin 1988
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_0T79
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 1mM Sodium Pyruvate + 0.2mM Serine + 0.2mM Aspartate
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 2
User's Publication 1

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3503  Yang Z, Kodama S, Suzuki K, Watanabe M.  Telomerase activity, telomere length, and chromosome aberrations in the extension of life span of human embryo cells induced by low-dose X-rays.  J Radiat Res  1998  39(1):35-51  PubMed ID: 9610031   DOI: 10.1269/jrr.39.35
3533  Watanabe M, Suzuki K, Kodama S, Sugahara T.  Normal human cells at confluence get heat resistance by efficient accumulation of hsp72 in nucleus.  Carcinogenesis  1995  16(10):2373-80  PubMed ID: 7586138   DOI: 10.1093/carcin/16.10.2373

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User's Publication
7593  Upadhyay AK, Rotili D, Han JW, Hu R, Chang Y, Labella D, Zhang X, Yoon YS, Mai A, Cheng X.  An analog of BIX-01294 selectively inhibits a family of histone H3 lysine 9 Jumonji demethylases.  J. Mol. Biol.  2012  416:319-27  PubMed ID: 22227394   DOI: 10.1016/j.jmb.2011.12.036

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