Cell No. : Cell Name
RCB2238 : HE18
update : 2023/10/16
|
| Comment | Human fibroblast-like cells derived from embryo. Not immortalized. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Watanabe, Masami
|
| Originator |
Watanabe, Masami
|
| Year of deposit |
2002
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Tissue |
embryo
|
| Classification |
normal
|
| Year of origin |
1988
|
|
Lifespan |
finite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_0T78
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM + 10% FBS + 1mM Sodium Pyruvate + 0.2mM Serine + 0.2mM Aspartate
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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|
| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
3503
Yang Z, Kodama S, Suzuki K, Watanabe M.
Telomerase activity, telomere length, and chromosome aberrations in the extension of life span of human embryo cells induced by low-dose X-rays.
J Radiat Res
1998
39(1):35-51
PubMed ID: 9610031
DOI: 10.1269/jrr.39.35
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|
3533
Watanabe M, Suzuki K, Kodama S, Sugahara T.
Normal human cells at confluence get heat resistance by efficient accumulation of hsp72 in nucleus.
Carcinogenesis
1995
16(10):2373-80
PubMed ID: 7586138
DOI: 10.1093/carcin/16.10.2373
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|
3534
Watanabe M, Suzuki M, Suzuki K, Nakano K, Watanabe K.
Effect of multiple irradiation with low doses of gamma-rays on morphological transformation and growth ability of human embryo cells in vitro.
Int J Radiat Biol
1992
62(6):711-8
PubMed ID: 1362764
DOI: 10.1080/09553009214552661
|
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