Cell No. : Cell Name
RCB2237 : HE16
update : 2023/10/16
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Comment | Human fibroblast-like cells derived from embryo. Not immortalized. |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Watanabe, Masami
|
Originator |
Watanabe, Masami
|
Year of deposit |
2002
|
Animal |
_human
< Mammals
|
Genus |
Homo
|
Species |
sapiens
|
Tissue |
embryo
|
Classification |
normal
|
Year of origin |
1988
|
|
Lifespan |
finite
|
Morphology |
fibroblast-like
|
Cellosaurus(Expasy) |
CVCL_0T77
|
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
|
Culture medium |
|
MEM + 10% FBS + 1mM Sodium Pyruvate + 0.2mM Serine + 0.2mM Aspartate
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Antibiotics |
|
Free
|
|
Passage method |
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0.25% Trypsin
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Culture information
|
Passage ratio |
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1 : 4 split
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SC frequency |
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Subculture : 2 times/week
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Temperature |
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37
℃
|
CO2 concentration |
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5
%
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Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Mycoplasma |
|
(-)
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Isozyme |
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LD, NP
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STR(human) |
|
OK
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Images |
deposit info | lot info |
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Reference information |
Reference |
1
|
User's Publication |
3
|
User's Publication |
19856
Fujibayashi S, Kiyono T, Endo Y, Tani T, Tate H, Bai L, Sugano E, Tomita H, Fukuda T.
Increased lentivirus titer using ultra expression vectors
Anal Biochem
2023
669:115119
PubMed ID: 36958509
DOI: 10.1016/j.ab.2023.115119
|
20475
Takada H, Miura T, Fujibayashi S, Sasaki N, Takahashi K, Sugano E, Tomita H, Ozaki T, Kiyono T, Yoshida MA, Fukuda T.
Detailed chromosome analysis of wild-type, immortalized fibroblasts with SV40T, E6E7, combinational introduction of cyclin dependent kinase 4, cyclin D1, telomerase reverse transcriptase.
In Vitro Cell Dev Biol Anim
2021
PubMed ID: 34888747
DOI: 10.1007/s11626-021-00631-0
|
14061
Fukuda T, Takahashi K, Takase S, Orimoto A, Eitsuka T, Nakagawa K, Kiyono T.
Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor
Front Cell Dev Biol
2020
8:157
PubMed ID: 32269992
DOI: 10.3389/fcell.2020.00157
|