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Cell No. : Cell Name
RCB2237 : HE16  update : 2023/10/16
CommentHuman fibroblast-like cells derived from embryo. Not immortalized.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Watanabe, Masami
Originator Watanabe, Masami
Year of deposit 2002
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue embryo
Classification normal
Year of origin 1988
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_0T77
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS + 1mM Sodium Pyruvate + 0.2mM Serine + 0.2mM Aspartate
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 3

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3503  Yang Z, Kodama S, Suzuki K, Watanabe M.  Telomerase activity, telomere length, and chromosome aberrations in the extension of life span of human embryo cells induced by low-dose X-rays.  J Radiat Res  1998  39(1):35-51  PubMed ID: 9610031   DOI: 10.1269/jrr.39.35

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User's Publication
19856  Fujibayashi S, Kiyono T, Endo Y, Tani T, Tate H, Bai L, Sugano E, Tomita H, Fukuda T.  Increased lentivirus titer using ultra expression vectors  Anal Biochem  2023  669:115119  PubMed ID: 36958509   DOI: 10.1016/j.ab.2023.115119
20475  Takada H, Miura T, Fujibayashi S, Sasaki N, Takahashi K, Sugano E, Tomita H, Ozaki T, Kiyono T, Yoshida MA, Fukuda T.  Detailed chromosome analysis of wild-type, immortalized fibroblasts with SV40T, E6E7, combinational introduction of cyclin dependent kinase 4, cyclin D1, telomerase reverse transcriptase.  In Vitro Cell Dev Biol Anim  2021    PubMed ID: 34888747   DOI: 10.1007/s11626-021-00631-0
14061  Fukuda T, Takahashi K, Takase S, Orimoto A, Eitsuka T, Nakagawa K, Kiyono T.  Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor  Front Cell Dev Biol  2020  8:157  PubMed ID: 32269992   DOI: 10.3389/fcell.2020.00157

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