Cell No. : Cell Name RCB2237 : HE16  update : 2023/10/16
Comment			Human fibroblast-like cells derived from embryo. Not immortalized.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Watanabe, Masami
		Originator	Watanabe, Masami
		Year of deposit	2002
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Tissue	embryo
		Classification	normal
		Year of origin	1988
		Lifespan	finite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_0T77
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM + 10% FBS + 1mM Sodium Pyruvate + 0.2mM Serine + 0.2mM Aspartate
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Isozyme		LD, NP
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	3

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Reference

3503  Yang Z, Kodama S, Suzuki K, Watanabe M.  Telomerase activity, telomere length, and chromosome aberrations in the extension of life span of human embryo cells induced by low-dose X-rays.  J Radiat Res  1998  39(1):35-51  PubMed ID: 9610031   DOI: 10.1269/jrr.39.35

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User's Publication

19856  Fujibayashi S, Kiyono T, Endo Y, Tani T, Tate H, Bai L, Sugano E, Tomita H, Fukuda T.  Increased lentivirus titer using ultra expression vectors  Anal Biochem  2023  669:115119  PubMed ID: 36958509   DOI: 10.1016/j.ab.2023.115119

20475  Takada H, Miura T, Fujibayashi S, Sasaki N, Takahashi K, Sugano E, Tomita H, Ozaki T, Kiyono T, Yoshida MA, Fukuda T.  Detailed chromosome analysis of wild-type, immortalized fibroblasts with SV40T, E6E7, combinational introduction of cyclin dependent kinase 4, cyclin D1, telomerase reverse transcriptase.  In Vitro Cell Dev Biol Anim  2021    PubMed ID: 34888747   DOI: 10.1007/s11626-021-00631-0

14061  Fukuda T, Takahashi K, Takase S, Orimoto A, Eitsuka T, Nakagawa K, Kiyono T.  Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor  Front Cell Dev Biol  2020  8:157  PubMed ID: 32269992   DOI: 10.3389/fcell.2020.00157