Cell No. : Cell Name RCB2230 : HS-Sch-2  update : 2021/02/26
Comment			Human cell line derived from malignant peripheral nerve sheath tumor (MPNST).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Sonobe, Hiroshi
		Originator	Sonobe, Hiroshi
		Year of deposit	2002
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Female
		Age at sampling	54 years
		Tissue	unknown
		Disease name	malignant peripheral nerve sheath tumour (MPNST)
		Classification	cancer
		Lifespan	infinite
		Morphology	other
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Medium and additives		DMEM (low glucose) + 10% FBS
		Antibiotics		Free
		Passage method		0.25% trypsin
	Culture information	Passage ratio		1 : 6-8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		STR(human)		OK
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	2

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Reference

3501  Sonobe H, Takeuchi T, Furihata M, Taguchi T, Kawai A, Ohjimi Y, Iwasaki H, Kaneko Y, Ohtsuki Y.  A new human malignant peripheral nerve sheath tumour-cell line, HS-sch-2, harbouring p53 point mutation.  Int J Oncol  2000  17(2):347-52  PubMed ID: 10891546

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User's Publication

10338  Fukushima S, Endo M, Matsumoto Y, Fukushi JI, Matsunobu T, Kawaguchi KI, Setsu N, IIda K, Yokoyama N, Nakagawa M, Yahiro K, Oda Y, Iwamoto Y, Nakashima Y.  Hypoxia-inducible factor 1 alpha is a poor prognostic factor and potential therapeutic target in malignant peripheral nerve sheath tumor.  PLoS ONE  2017  12:e0178064  PubMed ID: 28558056

9577  Tanaka T, Kikuchi N, Goto K, Iino M.  Sec6/8 regulates Bcl-2 and Mcl-1, but not Bcl-xl, in malignant peripheral nerve sheath tumor cells.  Apoptosis  2016  21:594-608  PubMed ID: 26892009