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Cell No. : Cell Name
RCB2230 : HS-Sch-2  update : 2018/07/06
CommentHuman cell line derived from malignant peripheral nerve sheath tumor (MPNST).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Sonobe, Hiroshi
Originator Sonobe, Hiroshi
Year of deposit 2002
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 54 years
Tissue unknown
Case history malignant peripheral nerve sheath tumour (MPNST)
Classification cancer
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 6-8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 2


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Reference
3501  Sonobe H, Takeuchi T, Furihata M, Taguchi T, Kawai A, Ohjimi Y, Iwasaki H, Kaneko Y, Ohtsuki Y.  A new human malignant peripheral nerve sheath tumour-cell line, HS-sch-2, harbouring p53 point mutation.  Int J Oncol  2000  17(2):347-52  PubMed ID: 10891546

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User's Publication
10338  Fukushima S, Endo M, Matsumoto Y, Fukushi JI, Matsunobu T, Kawaguchi KI, Setsu N, IIda K, Yokoyama N, Nakagawa M, Yahiro K, Oda Y, Iwamoto Y, Nakashima Y.  Hypoxia-inducible factor 1 alpha is a poor prognostic factor and potential therapeutic target in malignant peripheral nerve sheath tumor.  PLoS ONE  2017  12:e0178064  PubMed ID: 28558056
9577  Tanaka T, Kikuchi N, Goto K, Iino M.  Sec6/8 regulates Bcl-2 and Mcl-1, but not Bcl-xl, in malignant peripheral nerve sheath tumor cells.  Apoptosis  2016  21:594-608  PubMed ID: 26892009



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