Cell No. : Cell Name
RCB2229 : HS-Os-1
update : 2021/04/16
|
| Comment | Human cell line derived from osteosarcoma. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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|
|
Basic information
|
Depositor |
Sonobe, Hiroshi
|
| Originator |
Sonobe, Hiroshi
|
| Year of deposit |
2002
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
11 years
|
| Tissue |
left humerus
|
| Disease name |
osteosarcoma
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
other
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Medium and additives |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| STR(human) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
1
|
| User's Publication |
4
|
| User's Publication |
|
12455
Moritake H, Saito Y, Sawa D, Sameshima N, Yamada A, Kinoshita M, Kamimura S, Konomoto T, Nunoi H.
TAE226, a dual inhibitor of focal adhesion kinase and insulin-like growth factor-I receptor, is effective for Ewing sarcoma
Cancer Med
2019
8(18):7809-7821
PubMed ID: 31692287
|
|
10919
Antitumor activities of BIBF 1120, BI 860585, and BI 836845 in preclinical models of sarcoma
Integr Mol Med
2016
3: 755-760
|
|
7459
Tsuru A, Setoguchi T, Matsunoshita Y, Nagao-Kitamoto H, Nagano S, Yokouchi M, Maeda S, Ishidou Y, Yamamoto T, Komiya S.
Hairy/enhancer-of-split related with YRPW motif protein 1 promotes osteosarcoma metastasis via matrix metallopeptidase 9 expression.
Br. J. Cancer
2015
112:1232-40
PubMed ID: 25742474
|
|
6428
Arai K, Sakamoto R, Kubota D, Kondo T.
Proteomic approach toward molecular backgrounds of drug resistance of osteosarcoma cells in spheroid culture system.
Proteomics
2013
13(15):2351-60
PubMed ID: 23712969
|
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