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Cell No. : Cell Name
RCB2229 : HS-Os-1  update : 2021/04/16
CommentHuman cell line derived from osteosarcoma.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sonobe, Hiroshi
Originator Sonobe, Hiroshi
Year of deposit 2002
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 11 years
Tissue left humerus
Disease name osteosarcoma
Classification cancer
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 4

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3500  Sonobe H, Mizobuchi H, Manabe Y, Furihata M, Iwata J, Hikita T, Oka T, Ohtsuki Y, Goto T.  Morphological characterization of a newly established human osteosarcoma cell line, HS-Os-1, revealing its distinct osteoblastic nature.  Virchows Arch B Cell Pathol Incl Mol Pathol  1991  60(3):181-7  PubMed ID: 1679269

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User's Publication
12455  Moritake H, Saito Y, Sawa D, Sameshima N, Yamada A, Kinoshita M, Kamimura S, Konomoto T, Nunoi H.  TAE226, a dual inhibitor of focal adhesion kinase and insulin-like growth factor-I receptor, is effective for Ewing sarcoma  Cancer Med  2019  8(18):7809-7821  PubMed ID: 31692287
10919    Antitumor activities of BIBF 1120, BI 860585, and BI 836845 in preclinical models of sarcoma  Integr Mol Med  2016  3: 755-760 
7459  Tsuru A, Setoguchi T, Matsunoshita Y, Nagao-Kitamoto H, Nagano S, Yokouchi M, Maeda S, Ishidou Y, Yamamoto T, Komiya S.  Hairy/enhancer-of-split related with YRPW motif protein 1 promotes osteosarcoma metastasis via matrix metallopeptidase 9 expression.  Br. J. Cancer  2015  112:1232-40  PubMed ID: 25742474
6428  Arai K, Sakamoto R, Kubota D, Kondo T.  Proteomic approach toward molecular backgrounds of drug resistance of osteosarcoma cells in spheroid culture system.  Proteomics  2013  13(15):2351-60  PubMed ID: 23712969

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