Cell No. : Cell Name
RCB2229 : HS-Os-1
update : 2021/02/26
|
| Comment | Human cell line derived from osteosarcoma. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
|
|
|
Basic information
|
Depositor |
Sonobe, Hiroshi
|
| Originator |
Sonobe, Hiroshi
|
| Year of deposit |
2002
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
11 years
|
| Tissue |
left humerus
|
| Disease name |
osteosarcoma
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
other
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Medium and additives |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| STR(human) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
1
|
| User's Publication |
4
|
| User's Publication |
|
12455
Moritake H, Saito Y, Sawa D, Sameshima N, Yamada A, Kinoshita M, Kamimura S, Konomoto T, Nunoi H.
TAE226, a dual inhibitor of focal adhesion kinase and insulin-like growth factor-I receptor, is effective for Ewing sarcoma
Cancer Med
2019
8(18):7809-7821
PubMed ID: 31692287
|
|
10919
Antitumor activities of BIBF 1120, BI 860585, and BI 836845 in preclinical models of sarcoma
Integr Mol Med
2016
3: 755-760
|
|
7459
Tsuru A, Setoguchi T, Matsunoshita Y, Nagao-Kitamoto H, Nagano S, Yokouchi M, Maeda S, Ishidou Y, Yamamoto T, Komiya S.
Hairy/enhancer-of-split related with YRPW motif protein 1 promotes osteosarcoma metastasis via matrix metallopeptidase 9 expression.
Br. J. Cancer
2015
112:1232-40
PubMed ID: 25742474
|
|
6428
Arai K, Sakamoto R, Kubota D, Kondo T.
Proteomic approach toward molecular backgrounds of drug resistance of osteosarcoma cells in spheroid culture system.
Proteomics
2013
13(15):2351-60
PubMed ID: 23712969
|
Back
Top
CELL BANK Website