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Cell No. : Cell Name
RCB2225 : JHUEM-14  update : 2021/05/20
CommentHuman cell line derived from uterine endometrioid adenocarcinoma. Application consideration
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Contact Us
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.Cell Bank (ISHIKAWA Hiroshi)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ishikawa, Hiroshi
Originator Ishikawa, H. & Yasuda, M.
Year of deposit 2002
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 44 years
Tissue uterus
Disease name endometrioid adenocarcinoma of uterus
Classification cancer
Year of origin 2001
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4654
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 20% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 0
User's Publication 2

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User's Publication
12483  Baxter E, Windloch K, Kelly G, Lee JS, Gannon F, Brennan DJ.  Molecular basis of distinct oestrogen responses in endometrial and breast cancer  Endocr Relat Cancer  2019  26(1):31-46  PubMed ID: 30121621   DOI: 10.1530/ERC-17-0563
4261  Takeshita S, Yamashita Y, Shiomi K, Suzuki N, Yoshida J, Naiki-Ito A, Suzuki S, Akatsuka S, Toyokuni S, Takahashi T, Mase S, Arakawa A, Sugiura-Ogasawara M, Takahashi S.  Expression of P-REX2a is associated with poor prognosis in endometrial malignancies.  Oncotarget  2018    PubMed ID: 29872505   DOI: 10.18632/oncotarget.25349

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