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Cell No. : Cell Name
RCB2213 : NCC-RbC-60  update : 2024/01/17
CommentHuman cell line derived from retinoblastoma.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
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English
Address
Contact Us
cellbank.brc@riken.jp
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.KANEKO, Akihiro (Cell Bank)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kaneko, Akihiro
Originator Inomata, Masako
Year of deposit 2002
Another name R60
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 1 year
Tissue retinoblastoma
Disease name bilateral retinoblastoma
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_4073
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 20% FBS + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 2


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Reference
3455  Inomata M, Saijo N, Kawashima K, Kaneko A, Fujiwara Y, Kunikane H, Tanaka Y  Induction of apoptosis in cultured retinoblastoma cells by the protein phosphatase inhibitor, okadaic acid.  J Cancer Res Clin Oncol  1995  121:729-38  PubMed ID: 7499444   DOI: 10.1007/BF01213319
901  M Inomata, A Kaneko, N Saijo, S Tokura  Culture of retinoblastoma cells from clinical specimens: growth-promoting effect of 2-mercaptoethanol  J Cancer Res Clin Oncol  1994  120(3):149-55  PubMed ID: 8263010   DOI: 10.1007/BF01202193

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User's Publication
18226  Maradani BS, Parameswaran S, Subramanian K.  Development and characterization of DNA aptamer against Retinoblastoma by Cell-SELEX  Sci Rep  2022  12(1):16178  PubMed ID: 36171412   DOI: 10.1038/s41598-022-20660-3
13207  Togashi K, Okada M, Suzuki S, Sanomachi T, Seino S, Yamamoto M, Yamashita H, Kitanaka C.  Inhibition of Retinoblastoma Cell Growth by CEP1347 Through Activation of the P53 Pathway.  Anticancer Res  2020    PubMed ID: 32878784   DOI: 10.21873/anticanres.14499


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