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Cell No. : Cell Name
RCB2208 : NCC-RbC-54  update : 2023/01/04
CommentHuman cell line derived from retinoblastoma.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
RIKEN BioResource Research Center, Cell Bank, 3-1-1 Koyadai,Tsukuba-shi, Ibaraki 305-0074 Japan
Dr.KANEKO Akihiro
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kaneko, Akihiro
Originator Inomata, Masako
Year of deposit 2002
Another name R54
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 0.3 years
Tissue retinoblastoma
Disease name bilateral retinoblastoma
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_3049
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 20% FBS + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Reference information Reference 1
User's Publication 1

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901  M Inomata, A Kaneko, N Saijo, S Tokura  Culture of retinoblastoma cells from clinical specimens: growth-promoting effect of 2-mercaptoethanol  J Cancer Res Clin Oncol  1994  120(3):149-55  PubMed ID: 8263010   DOI: 10.1007/BF01202193

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User's Publication
13207  Togashi K, Okada M, Suzuki S, Sanomachi T, Seino S, Yamamoto M, Yamashita H, Kitanaka C.  Inhibition of Retinoblastoma Cell Growth by CEP1347 Through Activation of the P53 Pathway.  Anticancer Res  2020    PubMed ID: 32878784   DOI: 10.21873/anticanres.14499

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