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Cell No. : Cell Name
RCB2206 : NCC-RbC-51  update : 2024/08/14
CommentHuman retinoblastoma cell line derived from cervical lymph node metastasis.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
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English
Address
Contact Us
cellbank.brc@riken.jp
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.KANEKO, Akihiro (Cell Bank)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kaneko, Akihiro
Originator Inomata, Masako
Year of deposit 2002
Another name R51
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 4 years
Tissue retinoblastoma, cervical lymph node meta
Disease name bilateral retinoblastoma
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_3048
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 20% FBS + 1mM Sodium Pyruvate
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 2-6 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 9


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Reference
4081  Masato Maekawa, Motoko Inomata, Masao S Sasaki, Akihiro Kaneko, Mineko Ushiama, Kokichi Sugano, Jun Takayama, Takashi Kanno  Electrophoretic variant of a lactate dehydrogenase isoenzyme and selective promoter methylation of the LDHA gene in a human retinoblastoma cell line  Clin Chem  2002  48(11):1938-45  PubMed ID: 12406979  
3455  Inomata M, Saijo N, Kawashima K, Kaneko A, Fujiwara Y, Kunikane H, Tanaka Y  Induction of apoptosis in cultured retinoblastoma cells by the protein phosphatase inhibitor, okadaic acid.  J Cancer Res Clin Oncol  1995  121:729-38  PubMed ID: 7499444   DOI: 10.1007/BF01213319
901  M Inomata, A Kaneko, N Saijo, S Tokura  Culture of retinoblastoma cells from clinical specimens: growth-promoting effect of 2-mercaptoethanol  J Cancer Res Clin Oncol  1994  120(3):149-55  PubMed ID: 8263010   DOI: 10.1007/BF01202193

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User's Publication
21579  Khade OS, Sasidharan S, Jain A, Maradani BS, Chatterjee A, Gopal D, Ravi Kumar RK, Krishnakumar S, Pandey A, Janakiraman N, Elchuri SV, Gundimeda S.  Identification of dysregulation of sphingolipids in retinoblastoma using liquid chromatography-mass spectrometry.  Exp Eye Res  2024  109798  PubMed ID: 38246332   DOI: 10.1016/j.exer.2024.109798
17716  Gopal Divya, Ramani Madhura, Vikas Khetan, Pukhraj Rishi, Janakiraman Narayanan  Understanding the mechano and chemo response of retinoblastoma tumor cells  OpenNano  2022  8:100092    DOI: 10.1016/j.onano.2022.100092
18226  Maradani BS, Parameswaran S, Subramanian K.  Development and characterization of DNA aptamer against Retinoblastoma by Cell-SELEX  Sci Rep  2022  12(1):16178  PubMed ID: 36171412   DOI: 10.1038/s41598-022-20660-3
21035  Gopal D, Nagarajan H, Muthuvel B, Vetrivel U, George R, Janakiraman N.  Synthesis and Characterization of a Novel Peptide Targeting Human Tenon Fibroblast Cells To Modulate Fibrosis: An Integrated Empirical Approach.  ACS Pharmacol Transl Sci  2022  5(12):1254-1266  PubMed ID: 36524010   DOI: 10.1021/acsptsci.2c00148
17040  Sandeep Bose, Amit Chatterjee, Shantha Kumar Jenifer, Biswajit Mondal, Pillalamarri Srikrishnarka, Debasmita Ghosh, Angshuman Ray Chowdhuri, M. P. Kannan, Sailaja V. Elchuri, Thalappil Pradeep  Molecular Materials through Microdroplets: Synthesis of Protein-Protected Luminescent Clusters of Noble Metals  ACS Sustainable Chemistry & Engineering  2021  9:4554-4563    DOI: 10.1021/acssuschemeng.0c09145
13082  Raguraman R, Parameswaran S, Kanwar JR, Vasudevan M, Chitipothu S, Kanwar RK, Krishnakumar S.  Gene expression profiling of tumor stroma interactions in retinoblastoma.  Exp Eye Res  2020    PubMed ID: 32585195   DOI: 10.1016/j.exer.2020.108067
13774  Ravishankar H, Mangani AS, Shankar MB, Joshi M, Devasena T, Parameswaran S, Subramaniam K.  Characterization of NCC-RbC-51, an RB cell line isolated from a metastatic site  Histochem Cell Biol  2020  153(2):101-109  PubMed ID: 31781967   DOI: 10.1007/s00418-019-01832-1
13939  Ravishankar H, Mangani AS, Phoebe Moses GL, Mani SP, Parameswaran S, Khetan V, Ganesan S, Krishnakumar S.  Serum exosomal miRNA as biomarkers for Retinoblastoma  Exp Eye Res  2020  108184  PubMed ID: 32795526   DOI: 10.1016/j.exer.2020.108184
12528  Amit C, Muralikumar S, Janaki S, Lakshmipathy M, Therese KL, Umashankar V, Padmanabhan P, Narayanan J.  Designing and enhancing the antifungal activity of corneal specific cell penetrating peptide using gelatin hydrogel delivery system  Int J Nanomedicine  2019  14:605-622.  PubMed ID: 30697045   DOI: 10.2147/IJN.S184911


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