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Cell No. : Cell Name
RCB2206 : NCC-RbC-51  update : 2021/02/26
CommentHuman retinoblastoma cell line derived from cervical lymph node metastasis.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
RIKEN BioResource Research Center, Cell Bank, 3-1-1 Koyadai,Tsukuba-shi, Ibaraki 305-0074 Japan
Dr.Kaneko, Akihiro
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kaneko, Akihiro
Originator Inomata, Masako
Year of deposit 2002
Another name R51
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 4 years
Tissue retinoblastoma, cervical lymph node meta
Disease name bilateral retinoblastoma
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 20% FBS + 1mM Sodium Pyruvate
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 2-6 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 3


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Reference
4081  Masato Maekawa, Motoko Inomata, Masao S Sasaki, Akihiro Kaneko, Mineko Ushiama, Kokichi Sugano, Jun Takayama, Takashi Kanno  Electrophoretic variant of a lactate dehydrogenase isoenzyme and selective promoter methylation of the LDHA gene in a human retinoblastoma cell line  Clin Chem  2002  48(11):1938-45  PubMed ID: 12406979
3455  Inomata M, Saijo N, Kawashima K, Kaneko A, Fujiwara Y, Kunikane H, Tanaka Y  Induction of apoptosis in cultured retinoblastoma cells by the protein phosphatase inhibitor, okadaic acid.  J Cancer Res Clin Oncol  1995  121:729-38  PubMed ID: 7499444
3354  Inomata M, Kaneko A, Saijo N, Tokura S  Culture of retinoblastoma cells from clinical specimens: growth-promoting effect of 2-mercaptoethanol.  J Cancer Res Clin Oncol  1994  120:149-55  PubMed ID: 8263010

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User's Publication
13082  Raguraman R, Parameswaran S, Kanwar JR, Vasudevan M, Chitipothu S, Kanwar RK, Krishnakumar S.  Gene expression profiling of tumor stroma interactions in retinoblastoma.  Exp Eye Res  2020    PubMed ID: 32585195
13774  Ravishankar H, Mangani AS, Shankar MB, Joshi M, Devasena T, Parameswaran S, Subramaniam K.  Characterization of NCC-RbC-51, an RB cell line isolated from a metastatic site  Histochem Cell Biol  2020  153(2):101-109  PubMed ID: 31781967
12528  Amit C, Muralikumar S, Janaki S, Lakshmipathy M, Therese KL, Umashankar V, Padmanabhan P, Narayanan J.  Designing and enhancing the antifungal activity of corneal specific cell penetrating peptide using gelatin hydrogel delivery system  Int J Nanomedicine  2019  14:605-622.  PubMed ID: 30697045



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