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Cell No. : Cell Name
RCB2206 : NCC-RbC-51  update : 2018/07/06
CommentHuman retinoblastoma cell line derived from cervical lymph node metastasis.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
RIKEN BioResource Research Center, Cell Bank, 3-1-1 Koyadai,Tsukuba-shi, Ibaraki 305-0074 Japan
Dr.Kaneko, Akihiro
Fax. +81-29-836-9130
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Kaneko, Akihiro
Originator Inomata, Masako
Year of deposit 2002
Another name R51
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 4 years
Tissue retinoblastoma, cervical lymph node meta
Case history bilateral retinoblastoma
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 20% FBS + 1mM Sodium Pyruvate
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 2-6 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 0

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3455  Inomata M, Saijo N, Kawashima K, Kaneko A, Fujiwara Y, Kunikane H, Tanaka Y  Induction of apoptosis in cultured retinoblastoma cells by the protein phosphatase inhibitor, okadaic acid.  J Cancer Res Clin Oncol  1995  121:729-38  PubMed ID: 7499444
3354  Inomata M, Kaneko A, Saijo N, Tokura S  Culture of retinoblastoma cells from clinical specimens: growth-promoting effect of 2-mercaptoethanol.  J Cancer Res Clin Oncol  1994  120:149-55  PubMed ID: 8263010

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