Cell No. : Cell Name
RCB2204 : TFS-4
update : 2024/01/17
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| Comment | Hybridoma producing monoclonal antibody against a surface antigen of small cell carcinoma of lung. |
| Comment from the depositor | |
| Terms and conditions | 1) The RECIPIENT must contact theDEPOSITOR in the case of application for any patents or commercial usebased on the results from the use of the BIOLOGICAL RESOURSE. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Okabe, Tetsuro
|
| Originator |
Okabe, Tetsuro
|
| Year of deposit |
2005
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_J859
|
|
hybridoma
|
Fusion_cell_1 name |
P3U1
|
| Fusion_cell_1 strain |
mouse
|
| Fusion_cell_2 name |
spleen cell
|
| Fusion_cell_2 strain |
mouse, BALB/c
|
|
Fusion method |
PEG
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|
Antibody name |
TFS-4
|
| Subclass |
IgG1
|
| Immunogen |
Human Small cell lung carcinoma
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|
Antigen_name |
TFS-4
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| Antigen_m.w. |
124000
|
| Antigen_character |
Surface antigen of small cell carcinoma
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|
Antigen_positive |
Lung cancer, Neuroblastoma
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| Antigen_negative |
Stomach cancer, Pancreas cancer, Uterus cancer
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
|
RPMI1640 + 10% FBS
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| Antibiotics |
|
Free
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|
Passage method |
|
dilution
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Culture information
|
Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
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| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Antibody subclass |
|
IgG1
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| Reference information |
Reference |
0
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| User's Publication |
0
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