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Cell No. : Cell Name
RCB2203 : TFS-2  update : 2021/05/20
CommentHybridoma producing monoclonal antibody against a surface antigen of small cell carcinoma of lung.
Comment from the depositor
Terms and conditions1) The RECIPIENT must contact theDEPOSITOR in the case of application for any patents or commercial usebased on the results from the use of the BIOLOGICAL RESOURSE.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Okabe, Tetsuro
Originator Okabe, Tetsuro
Year of deposit 2005
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_J858
hybridoma Fusion_cell_1 name P3U1
Fusion_cell_1 strain mouse
Fusion_cell_2 name spleen cell
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG
Antibody name TFS-2
Subclass IgG2b
Immunogen Human Small cell lung carcinoma
Antigen_name TFS-2
Antigen_m.w. 39000
Antigen_character Surface antigen of small cell carcinoma
Antigen_positive Lung cancer, Stomach cancer, Pancreas cancer, Uterus cancer
Antigen_negative Peripheral blood cells, mononuclear luekocytes, Fibroblasts, Bone marrow mononuclear cells
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2b
Reference information Reference 0
User's Publication 0


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