細胞番号 : 細胞名 RCB2161 : UE7T-13  update : 2024/06/04
細胞特性(Comment:英)			Human mesenchymal cell line derived from bone marrow. Immortalized by HPV E7 and hTERT. RCB2152- RCB2163 are derived from the same bone marrow.
細胞特性(日)			ヒト間葉系細胞株。骨髄由来。HPV E7及びhTERTにて不死化。RCB2152- RCB2163は、同一骨髄由来。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)			基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英)			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日)			依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf
			提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料			手数料とお支払いについてはこちらをご覧ください。
細胞基本情報		寄託者	Umezawa, Akihiro
		樹立者	Umezawa, Akihiro
		寄託日	2005
		動物種	_human < Mammals
		属名	Homo
		種名	sapiens
		採取組織	bone marrow
		細胞分類	transformed
		遺伝子改変	recombinant
		外来遺伝子	LXSN-E7, LXSH-hTERT
		細胞寿命	infinite
		細胞形態	fibroblast-like
		Cellosaurus(Expasy)	CVCL_3238
細胞培養・検査情報			寄託時情報	ロット情報
	培地・試薬情報		培地・試薬一覧はこちらをご覧ください。
		培養形態		Adherent cells
		培地		POWEREDBY10
		抗生物質		Free
		継代方法		0.25% Trypsin
		播種細胞数		3 x 10 5 cells/100 mm dish
		継代・培地交換頻度		Subculture or Medium Renewal : once/3 days
		培養最適温度		37 ℃
		二酸化炭素濃度		5 %
		凍結培地		Medium + 10% DMSO
		凍結方法		Slow freezing
		マイコプラズマ/アコレプラズマ		(-)
		動物種PCR		OK
		アイソザイム検査		LD, NP
		個体識別検査		OK
文献情報		Reference(英)	3件
		Reference(日)	0件
		利用者成果(英)	16件
		利用者成果(日)	0件

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Reference(英)

4026  Mori T, Kiyono T, Imabayashi H, Takeda Y, Tsuchiya K, Miyoshi S, Makino H, Matsumoto K, Saito H, Ogawa S, Sakamoto M, Hata J, Umezawa A.  Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.  Mol Cell Biol  2005  25:5183-5195  PubMed ID: 15923633   DOI: 10.1128/MCB.25.12.5183-5195.2005

4027  Takeda Y, Mori T, Imabayashi H, Kiyono T, Gojo S, Miyoshi S, Hida N, Ita M, Segawa K, Ogawa S, Sakamoto M, Nakamura S, Umezawa A.  Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation?  J Gene Med  2004  6:833-845  PubMed ID: 15293342   DOI: 10.1002/jgm.583

4028  Imabayashi H, Mori T, Gojo S, Kiyono T, Sugiyama T, Irie R, Isogai T, Hata J, Toyama Y, Umezawa A.  Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis  Exp Cell Res  2003  288:35-50  PubMed ID: 12878157   DOI: 10.1016/s0014-4827(03)00130-7

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Reference(日)

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利用者成果(英)

22155  Peng Y, Iwasaki K, Taguchi Y, Ishikawa I, Umeda M.  Mesenchymal stem cell-derived protein extract induces periodontal regeneration.  Cytotherapy  2024    PubMed ID: 39545910   DOI: 10.1016/j.jcyt.2024.10.003

19602  Elif Beyza Koc, Bilgesu Onur Sucu  Synthesis and Antiproliferative Evaluation of New 1,3,5‐Trisubstituted 1,2,4‐Triazole Derivatives against Glioblastoma Cell Lines  ChemistrySelect  2023  8    DOI: 10.1002/slct.202300411

21857  Sucu BO, Koç EB.  Synthesis of novel oxadiazole derivatives and their cytotoxic activity against various cancer cell lines  Turk J Chem  2022  46(4):1089-1096  PubMed ID: 37538768   DOI: 10.55730/1300-0527.3417

17190  Karakaş N, Kiliç Ü.  Integrin α5β1 Mediated Cellular Reorganization in Human Mesenchymal Stem Cells During Neuronal Differentiation  In Vivo  2021  35(4):2127-213  PubMed ID: 34182488   DOI: 10.21873/invivo.12482

22331  Karakaş N, Bay S, Türkel N, Öztunç N, Öncül M, Bilgen H, Shah K, Şahin F, Öztürk G.  Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity  PLoS One  2020  15(5):e0228510  PubMed ID: 32407317   DOI: 10.1371/journal.pone.0228510

12603  Xie PY, Hu XJ, Guo RM, Meng XC, Pang PF, Zhou ZY, Li D, Shan H.  Generation of functional hepatocyte-like cells from human bone marrow mesenchymal stem cells by overexpression of transcription factor HNF4α and FOXA2  Hepatobiliary Pancreat Dis Int  2019  18(6):546-556  PubMed ID: 31230960   DOI: 10.1016/j.hbpd.2019.03.013

11051    Enhanced Multi-Lineage Differentiation of Human Mesenchymal Stem/Stromal Cells within Poly (N-isopropylacrylamide-Acrylic Acid) Microgel-Formed Three-Dimensional Constructs  J. Mater. Chem. B  2018  6, 1799-1814  PubMed ID: 32254252   DOI: 10.1039/C8TB00376A

4510  Guo R, Li Q, Yang F, Hu X, Jiao J, Guo Y, Wang J, Zhang Y.  In Vivo MR Imaging of Dual MRI Reporter Genes and Deltex-1 Gene-modified Human Mesenchymal Stem Cells in the Treatment of Closed Penile Fracture.  Mol Imaging Biol  2018  20(3):417-427  PubMed ID: 28971290   DOI: 10.1007/s11307-017-1128-0

14598  Yoshioka J, Ohsugi Y, Yoshitomi T, Yasukawa T, Sasaki N, Yoshimoto K.  Label-Free Rapid Separation and Enrichment of Bone Marrow-Derived Mesenchymal Stem Cells from a Heterogeneous Cell Mixture Using a Dielectrophoresis Device  Sensors (Basel)  2018  18(9):3007  PubMed ID: 30205546   DOI: 10.3390/s18093007

15055  Rovshen Akmammedov, Merve Huysal, Sevim Isik, Mehmet Senel  Preparation and characterization of novel chitosan/zeolite scaffolds for bone tissue engineering applications  International Journal of Polymeric Materials and Polymeric Biomaterials  2018  67:110-118    DOI: 10.1080/00914037.2017.1309539

22333  Zaim M, Isik S.  DNA topoisomerase IIβ stimulates neurite outgrowth in neural differentiated human mesenchymal stem cells through regulation of Rho-GTPases (RhoA/Rock2 pathway) and Nurr1 expression  Stem Cell Res Ther  2018  9(1):114  PubMed ID: 29695291   DOI: 10.1186/s13287-018-0859-4

10014  Fitter S, Gronthos S, Ooi SS, Zannettino AC.  The Mesenchymal Precursor Cell Marker Antibody STRO-1 Binds to Cell Surface Heat Shock Cognate 70.  Stem Cells  2017  35:940-951  PubMed ID: 28026090   DOI: 10.1002/stem.2560

9984  Akira Sasaki, Kazuyuki Hiwatashi, Masanori Kumagai, Keishi Hata, Masayuki Kobayashi  Relationships Between the Expression of Hepatocyte Nuclear Factors and Factors Essential for Lipoprotein Production in a Human Mesenchymal Stem Cell Line, UE7T-13  Biosci Biotechnol Biochem  2017  81(2):262-270  PubMed ID: 27838959   DOI: 10.1080/09168451.2016.1254530

13980  Liu JJ, Hu XJ, Li ZR, Yan RH, Li D, Wang J, Shan H.  In Vivo Bioluminescence Imaging of Transplanted Mesenchymal Stromal Cells and Their Rejection Mediated by Intrahepatic NK Cells  Mol Imaging Biol  2017  9(1):31-40  PubMed ID: 27406089   DOI: 10.1007/s11307-016-0962-9

14221  Terzioglu-Usak S, Negis Y, Karabulut DS, Zaim M, Isik S.  Cellular Model of Alzheimer's Disease: Aβ1-42 Peptide Induces Amyloid Deposition and a Decrease in Topo Isomerase IIβ and Nurr1 Expression  Curr Alzheimer Res  2017  14(6):636-644  PubMed ID: 28124588   DOI: 10.2174/1567205014666170117103217

15044  Li Z, Hu X, Mao J, Liu X, Zhang L, Liu J, Li D, Shan H.  Optimization of mesenchymal stem cells (MSCs) delivery dose and route in mice with acute liver injury by bioluminescence imaging  Mol Imaging Biol  2015  17(2):185-94  PubMed ID: 25273323   DOI: 10.1007/s11307-014-0792-6

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