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Cell No. : Cell Name
RCB2161 : UE7T-13  update : 2022/05/10
CommentHuman mesenchymal cell line derived from bone marrow. Immortalized by HPV E7 and hTERT. RCB2152- RCB2163 are derived from the same bone marrow.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2005
Animal human < Mammals
Genus Homo
Species sapiens
Tissue bone marrow
Classification transformed
Recombinant recombinant
Exogene LXSN-E7, LXSH-hTERT
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3238
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives POWEREDBY10
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 3 x 10^(5) cells/100 mm dish
SC frequency Subculture or Medium Renewal : once/3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
STR(human) OK
Reference information Reference 3
User's Publication 11


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Reference
4026  Mori T, Kiyono T, Imabayashi H, Takeda Y, Tsuchiya K, Miyoshi S, Makino H, Matsumoto K, Saito H, Ogawa S, Sakamoto M, Hata J, Umezawa A.  Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.  Mol Cell Biol  2005  25:5183-5195  PubMed ID: 15923633  
4027  Takeda Y, Mori T, Imabayashi H, Kiyono T, Gojo S, Miyoshi S, Hida N, Ita M, Segawa K, Ogawa S, Sakamoto M, Nakamura S, Umezawa A.  Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation?  J Gene Med  2004  6:833-845  PubMed ID: 15293342  
4028  Imabayashi H, Mori T, Gojo S, Kiyono T, Sugiyama T, Irie R, Isogai T, Hata J, Toyama Y, Umezawa A.  Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis  Exp Cell Res  2003  288:35-50  PubMed ID: 12878157  

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User's Publication
17190  Karakaş N, Kiliç Ü.  Integrin α5β1 Mediated Cellular Reorganization in Human Mesenchymal Stem Cells During Neuronal Differentiation  In Vivo  2021  35(4):2127-213  PubMed ID: 34182488   DOI: 10.21873/invivo.12482
12603  Xie PY, Hu XJ, Guo RM, Meng XC, Pang PF, Zhou ZY, Li D, Shan H.  Generation of functional hepatocyte-like cells from human bone marrow mesenchymal stem cells by overexpression of transcription factor HNF4α and FOXA2  Hepatobiliary Pancreat Dis Int  2019  18(6):546-556  PubMed ID: 31230960   DOI: 10.1016/j.hbpd.2019.03.013
11051    Enhanced Multi-Lineage Differentiation of Human Mesenchymal Stem/Stromal Cells within Poly (N-isopropylacrylamide-Acrylic Acid) Microgel-Formed Three-Dimensional Constructs  J. Mater. Chem. B  2018  6, 1799-1814  PubMed ID: 32254252   DOI: 10.1039/C8TB00376A
4510  Guo R, Li Q, Yang F, Hu X, Jiao J, Guo Y, Wang J, Zhang Y.  In Vivo MR Imaging of Dual MRI Reporter Genes and Deltex-1 Gene-modified Human Mesenchymal Stem Cells in the Treatment of Closed Penile Fracture.  Mol Imaging Biol  2018  20(3):417-427  PubMed ID: 28971290   DOI: 10.1007/s11307-017-1128-0
14598  Yoshioka J, Ohsugi Y, Yoshitomi T, Yasukawa T, Sasaki N, Yoshimoto K.  Label-Free Rapid Separation and Enrichment of Bone Marrow-Derived Mesenchymal Stem Cells from a Heterogeneous Cell Mixture Using a Dielectrophoresis Device  Sensors (Basel)  2018  18(9):3007  PubMed ID: 30205546   DOI: 10.3390/s18093007
15055  Rovshen Akmammedov, Merve Huysal, Sevim Isik, Mehmet Senel  Preparation and characterization of novel chitosan/zeolite scaffolds for bone tissue engineering applications  International Journal of Polymeric Materials and Polymeric Biomaterials  2018  67:110-118    DOI: 10.1080/00914037.2017.1309539
10014  Fitter S, Gronthos S, Ooi SS, Zannettino AC.  The Mesenchymal Precursor Cell Marker Antibody STRO-1 Binds to Cell Surface Heat Shock Cognate 70.  Stem Cells  2017  35:940-951  PubMed ID: 28026090   DOI: 10.1002/stem.2560
9984  Akira Sasaki, Kazuyuki Hiwatashi, Masanori Kumagai, Keishi Hata, Masayuki Kobayashi  Relationships Between the Expression of Hepatocyte Nuclear Factors and Factors Essential for Lipoprotein Production in a Human Mesenchymal Stem Cell Line, UE7T-13  Biosci Biotechnol Biochem  2017  81(2):262-270  PubMed ID: 27838959   DOI: 10.1080/09168451.2016.1254530
13980  Liu JJ, Hu XJ, Li ZR, Yan RH, Li D, Wang J, Shan H.  In Vivo Bioluminescence Imaging of Transplanted Mesenchymal Stromal Cells and Their Rejection Mediated by Intrahepatic NK Cells  Mol Imaging Biol  2017  9(1):31-40  PubMed ID: 27406089   DOI: 10.1007/s11307-016-0962-9
14221  Terzioglu-Usak S, Negis Y, Karabulut DS, Zaim M, Isik S.  Cellular Model of Alzheimer's Disease: Aβ1-42 Peptide Induces Amyloid Deposition and a Decrease in Topo Isomerase IIβ and Nurr1 Expression  Curr Alzheimer Res  2017  14(6):636-644  PubMed ID: 28124588   DOI: 10.2174/1567205014666170117103217
15044  Li Z, Hu X, Mao J, Liu X, Zhang L, Liu J, Li D, Shan H.  Optimization of mesenchymal stem cells (MSCs) delivery dose and route in mice with acute liver injury by bioluminescence imaging  Mol Imaging Biol  2015  17(2):185-94  PubMed ID: 25273323   DOI: 10.1007/s11307-014-0792-6



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