Cell No. : Cell Name
RCB2161 : UE7T-13
update : 2024/06/04
|
Comment | Human mesenchymal cell line derived from bone marrow. Immortalized by HPV E7 and hTERT. RCB2152- RCB2163 are derived from the same bone marrow. |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Umezawa, Akihiro
|
Originator |
Umezawa, Akihiro
|
Year of deposit |
2005
|
Animal |
_human
< Mammals
|
Genus |
Homo
|
Species |
sapiens
|
Tissue |
bone marrow
|
Classification |
transformed
|
Recombinant |
recombinant
|
Exogene |
LXSN-E7, LXSH-hTERT
|
|
Lifespan |
infinite
|
Morphology |
fibroblast-like
|
Cellosaurus(Expasy) |
CVCL_3238
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
Culture medium |
|
POWEREDBY10
|
Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage cell No |
|
3 x 10 5 cells/100 mm dish
|
SC frequency |
|
Subculture or Medium Renewal : once/3 days
|
Temperature |
|
37
℃
|
CO2 concentration |
|
5
%
|
Freeze medium |
|
Medium + 10% DMSO
|
Freezing method |
|
Slow freezing
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Mycoplasma |
|
(-)
|
Animal PCR |
|
OK
|
Isozyme |
|
LD, NP
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STR(human) |
|
OK
|
Reference information |
Reference |
3
|
User's Publication |
12
|
Reference |
4026
Mori T, Kiyono T, Imabayashi H, Takeda Y, Tsuchiya K, Miyoshi S, Makino H, Matsumoto K, Saito H, Ogawa S, Sakamoto M, Hata J, Umezawa A.
Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.
Mol Cell Biol
2005
25:5183-5195
PubMed ID: 15923633
DOI: 10.1128/MCB.25.12.5183-5195.2005
|
4027
Takeda Y, Mori T, Imabayashi H, Kiyono T, Gojo S, Miyoshi S, Hida N, Ita M, Segawa K, Ogawa S, Sakamoto M, Nakamura S, Umezawa A.
Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation?
J Gene Med
2004
6:833-845
PubMed ID: 15293342
DOI: 10.1002/jgm.583
|
4028
Imabayashi H, Mori T, Gojo S, Kiyono T, Sugiyama T, Irie R, Isogai T, Hata J, Toyama Y, Umezawa A.
Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis
Exp Cell Res
2003
288:35-50
PubMed ID: 12878157
DOI: 10.1016/s0014-4827(03)00130-7
|
User's Publication |
19602
Elif Beyza Koc, Bilgesu Onur Sucu
Synthesis and Antiproliferative Evaluation of New 1,3,5‐Trisubstituted 1,2,4‐Triazole Derivatives against Glioblastoma Cell Lines
ChemistrySelect
2023
8
DOI: 10.1002/slct.202300411
|
17190
Karakaş N, Kiliç Ü.
Integrin α5β1 Mediated Cellular Reorganization in Human Mesenchymal Stem Cells During Neuronal Differentiation
In Vivo
2021
35(4):2127-213
PubMed ID: 34182488
DOI: 10.21873/invivo.12482
|
12603
Xie PY, Hu XJ, Guo RM, Meng XC, Pang PF, Zhou ZY, Li D, Shan H.
Generation of functional hepatocyte-like cells from human bone marrow mesenchymal stem cells by overexpression of transcription factor HNF4α and FOXA2
Hepatobiliary Pancreat Dis Int
2019
18(6):546-556
PubMed ID: 31230960
DOI: 10.1016/j.hbpd.2019.03.013
|
11051
Enhanced Multi-Lineage Differentiation of Human Mesenchymal Stem/Stromal Cells within Poly (N-isopropylacrylamide-Acrylic Acid) Microgel-Formed Three-Dimensional Constructs
J. Mater. Chem. B
2018
6, 1799-1814
PubMed ID: 32254252
DOI: 10.1039/C8TB00376A
|
4510
Guo R, Li Q, Yang F, Hu X, Jiao J, Guo Y, Wang J, Zhang Y.
In Vivo MR Imaging of Dual MRI Reporter Genes and Deltex-1 Gene-modified Human Mesenchymal Stem Cells in the Treatment of Closed Penile Fracture.
Mol Imaging Biol
2018
20(3):417-427
PubMed ID: 28971290
DOI: 10.1007/s11307-017-1128-0
|
14598
Yoshioka J, Ohsugi Y, Yoshitomi T, Yasukawa T, Sasaki N, Yoshimoto K.
Label-Free Rapid Separation and Enrichment of Bone Marrow-Derived Mesenchymal Stem Cells from a Heterogeneous Cell Mixture Using a Dielectrophoresis Device
Sensors (Basel)
2018
18(9):3007
PubMed ID: 30205546
DOI: 10.3390/s18093007
|
15055
Rovshen Akmammedov, Merve Huysal, Sevim Isik, Mehmet Senel
Preparation and characterization of novel chitosan/zeolite scaffolds for bone tissue engineering applications
International Journal of Polymeric Materials and Polymeric Biomaterials
2018
67:110-118
DOI: 10.1080/00914037.2017.1309539
|
10014
Fitter S, Gronthos S, Ooi SS, Zannettino AC.
The Mesenchymal Precursor Cell Marker Antibody STRO-1 Binds to Cell Surface Heat Shock Cognate 70.
Stem Cells
2017
35:940-951
PubMed ID: 28026090
DOI: 10.1002/stem.2560
|
9984
Akira Sasaki, Kazuyuki Hiwatashi, Masanori Kumagai, Keishi Hata, Masayuki Kobayashi
Relationships Between the Expression of Hepatocyte Nuclear Factors and Factors Essential for Lipoprotein Production in a Human Mesenchymal Stem Cell Line, UE7T-13
Biosci Biotechnol Biochem
2017
81(2):262-270
PubMed ID: 27838959
DOI: 10.1080/09168451.2016.1254530
|
13980
Liu JJ, Hu XJ, Li ZR, Yan RH, Li D, Wang J, Shan H.
In Vivo Bioluminescence Imaging of Transplanted Mesenchymal Stromal Cells and Their Rejection Mediated by Intrahepatic NK Cells
Mol Imaging Biol
2017
9(1):31-40
PubMed ID: 27406089
DOI: 10.1007/s11307-016-0962-9
|
14221
Terzioglu-Usak S, Negis Y, Karabulut DS, Zaim M, Isik S.
Cellular Model of Alzheimer's Disease: Aβ1-42 Peptide Induces Amyloid Deposition and a Decrease in Topo Isomerase IIβ and Nurr1 Expression
Curr Alzheimer Res
2017
14(6):636-644
PubMed ID: 28124588
DOI: 10.2174/1567205014666170117103217
|
15044
Li Z, Hu X, Mao J, Liu X, Zhang L, Liu J, Li D, Shan H.
Optimization of mesenchymal stem cells (MSCs) delivery dose and route in mice with acute liver injury by bioluminescence imaging
Mol Imaging Biol
2015
17(2):185-94
PubMed ID: 25273323
DOI: 10.1007/s11307-014-0792-6
|