Cell No. : Cell Name RCB2160 : UE6E7T-12  update : 2023/10/16
Comment			Human mesenchymal cell line derived from bone marrow. Immortalized by HPV E6, E7, and hTERT. RCB2152- RCB2163 are derived from the same bone marrow.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Umezawa, Akihiro
		Originator	Umezawa, Akihiro
		Year of deposit	2005
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Tissue	bone marrow
		Classification	transformed
		Recombinant	recombinant
		Exogene	LXSN-E7, LXSH-hTERT, MSCVpuro-16E6SDD151
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_3235
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		POWEREDBY10
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage cell No		5 x 10 5 cells/100 mm dish
		SC frequency		Subculture or Medium Renewal : once/3 days
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP
		STR(human)		OK
Reference information		Reference	3
		User's Publication	1

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Reference

4026  Mori T, Kiyono T, Imabayashi H, Takeda Y, Tsuchiya K, Miyoshi S, Makino H, Matsumoto K, Saito H, Ogawa S, Sakamoto M, Hata J, Umezawa A.  Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.  Mol Cell Biol  2005  25:5183-5195  PubMed ID: 15923633   DOI: 10.1128/MCB.25.12.5183-5195.2005

4027  Takeda Y, Mori T, Imabayashi H, Kiyono T, Gojo S, Miyoshi S, Hida N, Ita M, Segawa K, Ogawa S, Sakamoto M, Nakamura S, Umezawa A.  Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation?  J Gene Med  2004  6:833-845  PubMed ID: 15293342   DOI: 10.1002/jgm.583

4028  Imabayashi H, Mori T, Gojo S, Kiyono T, Sugiyama T, Irie R, Isogai T, Hata J, Toyama Y, Umezawa A.  Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis  Exp Cell Res  2003  288:35-50  PubMed ID: 12878157   DOI: 10.1016/s0014-4827(03)00130-7

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User's Publication

14434  Mao H, Kim SM, Ueki M, Ito Y.  Serum-free culturing of human mesenchymal stem cells with immobilized growth factors  J Mater Chem B  2017  5(5):928-934  PubMed ID: 32263870   DOI: 10.1039/c6tb02867e