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Cell No. : Cell Name
RCB2160 : UE6E7T-12  update : 2023/10/16
CommentHuman mesenchymal cell line derived from bone marrow. Immortalized by HPV E6, E7, and hTERT. RCB2152- RCB2163 are derived from the same bone marrow.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2005
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue bone marrow
Classification transformed
Recombinant recombinant
Exogene LXSN-E7, LXSH-hTERT, MSCVpuro-16E6SDD151
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3235
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium POWEREDBY10
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 5 x 10 5 cells/100 mm dish
SC frequency Subculture or Medium Renewal : once/3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Isozyme LD, NP
STR(human) OK
Reference information Reference 3
User's Publication 1


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Reference
4026  Mori T, Kiyono T, Imabayashi H, Takeda Y, Tsuchiya K, Miyoshi S, Makino H, Matsumoto K, Saito H, Ogawa S, Sakamoto M, Hata J, Umezawa A.  Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.  Mol Cell Biol  2005  25:5183-5195  PubMed ID: 15923633   DOI: 10.1128/MCB.25.12.5183-5195.2005
4027  Takeda Y, Mori T, Imabayashi H, Kiyono T, Gojo S, Miyoshi S, Hida N, Ita M, Segawa K, Ogawa S, Sakamoto M, Nakamura S, Umezawa A.  Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation?  J Gene Med  2004  6:833-845  PubMed ID: 15293342   DOI: 10.1002/jgm.583
4028  Imabayashi H, Mori T, Gojo S, Kiyono T, Sugiyama T, Irie R, Isogai T, Hata J, Toyama Y, Umezawa A.  Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis  Exp Cell Res  2003  288:35-50  PubMed ID: 12878157   DOI: 10.1016/s0014-4827(03)00130-7

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User's Publication
14434  Mao H, Kim SM, Ueki M, Ito Y.  Serum-free culturing of human mesenchymal stem cells with immobilized growth factors  J Mater Chem B  2017  5(5):928-934  PubMed ID: 32263870   DOI: 10.1039/c6tb02867e



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