Cell No. : Cell Name
RCB2159 : UE6E7T-11
update : 2023/10/16
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| Comment | Human mesenchymal cell line derived from bone marrow. Immortalized by HPV E6, E7, and hTERT. RCB2152- RCB2163 are derived from the same bone marrow. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Umezawa, Akihiro
|
| Originator |
Umezawa, Akihiro
|
| Year of deposit |
2005
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Tissue |
bone marrow
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
LXSN-E7, LXSH-hTERT, MSCVpuro-16E6SDD151
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_3234
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
POWEREDBY10
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage cell No |
|
5 x 10 5 cells/100 mm dish
|
| SC frequency |
|
Subculture or Medium Renewal : once/3 days
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
4026
Mori T, Kiyono T, Imabayashi H, Takeda Y, Tsuchiya K, Miyoshi S, Makino H, Matsumoto K, Saito H, Ogawa S, Sakamoto M, Hata J, Umezawa A.
Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.
Mol Cell Biol
2005
25:5183-5195
PubMed ID: 15923633
DOI: 10.1128/MCB.25.12.5183-5195.2005
|
|
4027
Takeda Y, Mori T, Imabayashi H, Kiyono T, Gojo S, Miyoshi S, Hida N, Ita M, Segawa K, Ogawa S, Sakamoto M, Nakamura S, Umezawa A.
Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation?
J Gene Med
2004
6:833-845
PubMed ID: 15293342
DOI: 10.1002/jgm.583
|
|
4028
Imabayashi H, Mori T, Gojo S, Kiyono T, Sugiyama T, Irie R, Isogai T, Hata J, Toyama Y, Umezawa A.
Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis
Exp Cell Res
2003
288:35-50
PubMed ID: 12878157
DOI: 10.1016/s0014-4827(03)00130-7
|
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