Cell No. : Cell Name
RCB2154 : UE6E7T-3
update : 2025/08/13
|
| Comment | Human mesenchymal cell line derived from bone marrow. Immortalized by HPV E6, E7, and hTERT. RCB2152- RCB2163 are derived from the same bone marrow. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Umezawa, Akihiro
|
| Originator |
Umezawa, Akihiro
|
| Year of deposit |
2005
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Tissue |
bone marrow
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
LXSN-E7, LXSH-hTERT, MSCVpuro-16E6SDD151
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_3237
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
POWEREDBY10
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage cell No |
|
5 x 10 5 cells/100 mm dish
|
| SC frequency |
|
Subculture or Medium Renewal : once/3 days
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
3
|
| User's Publication |
13
|
| Reference |
|
4026
Mori T, Kiyono T, Imabayashi H, Takeda Y, Tsuchiya K, Miyoshi S, Makino H, Matsumoto K, Saito H, Ogawa S, Sakamoto M, Hata J, Umezawa A.
Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.
Mol Cell Biol
2005
25:5183-5195
PubMed ID: 15923633
DOI: 10.1128/MCB.25.12.5183-5195.2005
|
|
4027
Takeda Y, Mori T, Imabayashi H, Kiyono T, Gojo S, Miyoshi S, Hida N, Ita M, Segawa K, Ogawa S, Sakamoto M, Nakamura S, Umezawa A.
Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation?
J Gene Med
2004
6:833-845
PubMed ID: 15293342
DOI: 10.1002/jgm.583
|
|
4028
Imabayashi H, Mori T, Gojo S, Kiyono T, Sugiyama T, Irie R, Isogai T, Hata J, Toyama Y, Umezawa A.
Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis
Exp Cell Res
2003
288:35-50
PubMed ID: 12878157
DOI: 10.1016/s0014-4827(03)00130-7
|
| User's Publication |
|
19933
Li Y, Nie J, Wu Q, Yang X, Jiang P.
Circ-Sirt1 promotes osteoblast differentiation by activating Sirt1 and Wnt/β-catenin pathway
Acta Biochim Pol . 2023 Feb 25;70(1):51-57
2023
70(1):51-57
PubMed ID: 36840968
DOI: 10.18388/abp.2020_6035
|
|
17760
Kafi MA, Aktar K, Todo M, Dahiya R.
Engineered chitosan for improved 3D tissue growth through Paxillin-FAK-ERK activation
Regen Biomater
2020
7(2):141-151
PubMed ID: 32296533
DOI: 10.1093/rb/rbz034
|
|
10560
Md. Shariful Islam,A. M. Zahidur Rahman,M. H. Sharif,Alam Khan,Md. Abdulla-Al-Mamun ORCID Icon &Mitsugu Todo
Effects of compressive ratio and sintering temperature on mechanical properties of biocompatible collagen/hydroxyapatite composite scaffolds fabricated for bone tissue engineering
Journal of Asian Ceramic Societies
2019
7
DOI: 10.1080/21870764.2019.1600226
|
|
12601
Morita Y, Sato T, Higashiura K, Hirano Y, Matsubara F, Oshima K, Niwa K, Toku Y, Song G, Luo Q, Ju Y.
The optimal mechanical condition in stem cell-to-tenocyte differentiation determined with the homogeneous strain distributions and the cellular orientation control
Biol Open
2019
8(5):bio039164
PubMed ID: 31118166
DOI: 10.1242/bio.039164
|
|
12602
Nakamuta Y, Arahira T, Todo M.
Effects of culture conditions on the mechanical and biological properties of engineered cartilage constructed with collagen hybrid scaffold and human mesenchymal stem cells
J Mater Sci Mater Med
2019
30(10):119
PubMed ID: 31630248
DOI: 10.1007/s10856-019-6321-z
|
|
4446
Yasuyuki Morita*, Fuga Matsubara, Yuhki Toku, Yang Ju
Use of a Tendon/Ligament Cell Sheet with Mesenchymal Stem Cells and Cyclic Stretch Stimulus for Tendon/Ligament Tissue Restoration
Journal of Mechanical Engineering
2018
5, 1-11
|
|
10960
Highly sensitive and selective detection of bisphenol A based on reduced graphene oxide decorated hydroxyapatite nanocomposites
Electrochimica Acta
2017
241, 353-361
DOI: 10.1016/j.electacta.2017.04.135
|
|
10994
Yasuyuki Morita Naoki Kawase Takashi Yamauchi Yang Ju
Measurement of 3D Deformation Field of ECM Generated by Mesenchymal Stem Cell Using DVC Method
Mechanics of Biological Systems, Materials and other topics in Experimental and Applied Mechanics
2017
4:9-14
DOI: 10.1007/978-3-319-63552-1_2
|
|
14561
Pangesty AI, Arahira T, Todo M.
Development and characterization of hybrid tubular structure of PLCL porous scaffold with hMSCs/ECs cell sheet
J Mater Sci Mater Med
2017
28(10):165
PubMed ID: 28914404
DOI: 10.1007/s10856-017-5985-5
|
|
14586
Yusuke Nakamuta, Mitsugu Todo, Takaaki Arahira
Improvement of Collagen Gel/Sponge Composite Scaffold by Gel Wrapping for Cartilage Tissue Engineering
International Journal of Bioscience, Biochemistry and Bioinformatics
2017
7:102-109
DOI: 10.17706/ijbbb.2017.7.2.102-109
|
|
9588
Morita Y, Kawase N, Ju Y, Yamauchi T.
Mesenchymal stem cell-induced 3D displacement field of cell-adhesion matrices with differing elasticities.
J Mech Behav Biomed Mater
2016
60:394-400
PubMed ID: 26945874
DOI: 10.1016/j.jmbbm.2016.02.025
|
|
6425
Shariful Islam, Mitsugu Todo
Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering
American Journal of Clinical and Experimental Medicine
2015
3(5): 268-274
|
|
12241
Baiyao Xu, Yang Ju, Yanbin Cui, Guanbin Song
Carbon nanotube array inducing osteogenic differentiation of human mesenchymal stem cells
Mater Sci Eng C Mater Biol AppI
2015
51:182-8
PubMed ID: 25842124
DOI: 10.1016/j.msec.2015.02.052
|
Back
Top
CELL BANK Website