Back Back
Cell No. : Cell Name
RCB2154 : UE6E7T-3  update : 2023/10/16
CommentHuman mesenchymal cell line derived from bone marrow. Immortalized by HPV E6, E7, and hTERT. RCB2152- RCB2163 are derived from the same bone marrow.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2005
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue bone marrow
Classification transformed
Recombinant recombinant
Exogene LXSN-E7, LXSH-hTERT, MSCVpuro-16E6SDD151
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3237
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium POWEREDBY10
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 5 x 10 5 cells/100 mm dish
SC frequency Subculture or Medium Renewal : once/3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
STR(human) OK
Reference information Reference 3
User's Publication 13

To topTop
4026  Mori T, Kiyono T, Imabayashi H, Takeda Y, Tsuchiya K, Miyoshi S, Makino H, Matsumoto K, Saito H, Ogawa S, Sakamoto M, Hata J, Umezawa A.  Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.  Mol Cell Biol  2005  25:5183-5195  PubMed ID: 15923633   DOI: 10.1128/MCB.25.12.5183-5195.2005
4027  Takeda Y, Mori T, Imabayashi H, Kiyono T, Gojo S, Miyoshi S, Hida N, Ita M, Segawa K, Ogawa S, Sakamoto M, Nakamura S, Umezawa A.  Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation?  J Gene Med  2004  6:833-845  PubMed ID: 15293342   DOI: 10.1002/jgm.583
4028  Imabayashi H, Mori T, Gojo S, Kiyono T, Sugiyama T, Irie R, Isogai T, Hata J, Toyama Y, Umezawa A.  Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis  Exp Cell Res  2003  288:35-50  PubMed ID: 12878157   DOI: 10.1016/s0014-4827(03)00130-7

To topTop
User's Publication
19933  Li Y, Nie J, Wu Q, Yang X, Jiang P.  Circ-Sirt1 promotes osteoblast differentiation by activating Sirt1 and Wnt/β-catenin pathway  Acta Biochim Pol . 2023 Feb 25;70(1):51-57  2023  Acta Biochim Pol . 2023 Feb 25;70(1):51-57  PubMed ID: 36840968   DOI: 10.18388/abp.2020_6035
17760  Kafi MA, Aktar K, Todo M, Dahiya R.  Engineered chitosan for improved 3D tissue growth through Paxillin-FAK-ERK activation  Regen Biomater  2020  7(2):141-151  PubMed ID: 32296533   DOI: 10.1093/rb/rbz034
10560  Md. Shariful Islam,A. M. Zahidur Rahman,M. H. Sharif,Alam Khan,Md. Abdulla-Al-Mamun ORCID Icon &Mitsugu Todo  Effects of compressive ratio and sintering temperature on mechanical properties of biocompatible collagen/hydroxyapatite composite scaffolds fabricated for bone tissue engineering  Journal of Asian Ceramic Societies  2019  7    DOI: 10.1080/21870764.2019.1600226
12601  Morita Y, Sato T, Higashiura K, Hirano Y, Matsubara F, Oshima K, Niwa K, Toku Y, Song G, Luo Q, Ju Y.  The optimal mechanical condition in stem cell-to-tenocyte differentiation determined with the homogeneous strain distributions and the cellular orientation control  Biol Open  2019  8(5):bio039164  PubMed ID: 31118166   DOI: 10.1242/bio.039164
12602  Nakamuta Y, Arahira T, Todo M.  Effects of culture conditions on the mechanical and biological properties of engineered cartilage constructed with collagen hybrid scaffold and human mesenchymal stem cells  J Mater Sci Mater Med  2019  30(10):119  PubMed ID: 31630248   DOI: 10.1007/s10856-019-6321-z
4446  Yasuyuki Morita*, Fuga Matsubara, Yuhki Toku, Yang Ju  Use of a Tendon/Ligament Cell Sheet with Mesenchymal Stem Cells and Cyclic Stretch Stimulus for Tendon/Ligament Tissue Restoration  Journal of Mechanical Engineering  2018  5, 1-11   
10960    Highly sensitive and selective detection of bisphenol A based on reduced graphene oxide decorated hydroxyapatite nanocomposites  Electrochimica Acta  2017  241, 353-361    DOI: 10.1016/j.electacta.2017.04.135
10994  Yasuyuki Morita Naoki Kawase Takashi Yamauchi Yang Ju  Measurement of 3D Deformation Field of ECM Generated by Mesenchymal Stem Cell Using DVC Method  Mechanics of Biological Systems, Materials and other topics in Experimental and Applied Mechanics  2017  4:9-14    DOI: 10.1007/978-3-319-63552-1_2
14561  Pangesty AI, Arahira T, Todo M.  Development and characterization of hybrid tubular structure of PLCL porous scaffold with hMSCs/ECs cell sheet  J Mater Sci Mater Med  2017  28(10):165  PubMed ID: 28914404   DOI: 10.1007/s10856-017-5985-5
14586  Yusuke Nakamuta, Mitsugu Todo, Takaaki Arahira  Improvement of Collagen Gel/Sponge Composite Scaffold by Gel Wrapping for Cartilage Tissue Engineering  International Journal of Bioscience, Biochemistry and Bioinformatics  2017  7:102-109    DOI: 10.17706/ijbbb.2017.7.2.102-109
9588  Morita Y, Kawase N, Ju Y, Yamauchi T.  Mesenchymal stem cell-induced 3D displacement field of cell-adhesion matrices with differing elasticities.  J Mech Behav Biomed Mater  2016  60:394-400  PubMed ID: 26945874   DOI: 10.1016/j.jmbbm.2016.02.025
6425  Shariful Islam, Mitsugu Todo  Improved Mechanical Properties of PVA-Chitosan Polymeric Porous Scaffolds for Tissue Engineering   American Journal of Clinical and Experimental Medicine  2015  3(5): 268-274    
12241  Baiyao Xu, Yang Ju, Yanbin Cui, Guanbin Song  Carbon nanotube array inducing osteogenic differentiation of human mesenchymal stem cells  Mater Sci Eng C Mater Biol AppI  2015  51:182-8  PubMed ID: 25842124   DOI: 10.1016/j.msec.2015.02.052

Back Back Return Top Page