Cell No. : Cell Name
RCB2153 : UE6E7T-2
update : 2024/05/28
|
| Comment | Human mesenchymal cell line derived from bone marrow. Immortalized by HPV E6, E7, and hTERT. RCB2152- RCB2163 are derived from the same bone marrow. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Umezawa, Akihiro
|
| Originator |
Umezawa, Akihiro
|
| Year of deposit |
2005
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Tissue |
bone marrow
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
LXSN-E7, LXSH-hTERT, MSCVpuro-16E6SDD151
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_3236
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
POWEREDBY10
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage cell No |
|
5 x 10 5 cells/100 mm dish
|
| SC frequency |
|
Subculture or Medium Renewal : once/3 days
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
3
|
| User's Publication |
6
|
| Reference |
|
4026
Mori T, Kiyono T, Imabayashi H, Takeda Y, Tsuchiya K, Miyoshi S, Makino H, Matsumoto K, Saito H, Ogawa S, Sakamoto M, Hata J, Umezawa A.
Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.
Mol Cell Biol
2005
25:5183-5195
PubMed ID: 15923633
DOI: 10.1128/MCB.25.12.5183-5195.2005
|
|
4027
Takeda Y, Mori T, Imabayashi H, Kiyono T, Gojo S, Miyoshi S, Hida N, Ita M, Segawa K, Ogawa S, Sakamoto M, Nakamura S, Umezawa A.
Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation?
J Gene Med
2004
6:833-845
PubMed ID: 15293342
DOI: 10.1002/jgm.583
|
|
4028
Imabayashi H, Mori T, Gojo S, Kiyono T, Sugiyama T, Irie R, Isogai T, Hata J, Toyama Y, Umezawa A.
Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis
Exp Cell Res
2003
288:35-50
PubMed ID: 12878157
DOI: 10.1016/s0014-4827(03)00130-7
|
| User's Publication |
|
9959
Purroy N, Carabia J, Abrisqueta P, Egia L, Aguiló M, Carpio C, Palacio C, Crespo M, Bosch F.
Inhibition of BCR signaling using the Syk inhibitor TAK-659 prevents stroma-mediated signaling in chronic lymphocytic leukemia cells.
Oncotarget
2017
8:742-756
PubMed ID: 27888629
DOI: 10.18632/oncotarget.13557
|
|
14750
Carabia J, Carpio C, Abrisqueta P, Jiménez I, Purroy N, Calpe E, Palacio C, Bosch F, Crespo M.
Microenvironment regulates the expression of miR-21 and tumor suppressor genes PTEN, PIAS3 and PDCD4 through ZAP-70 in chronic lymphocytic leukemia
Sci Rep
2017
7(1):12262
PubMed ID: 28947822
DOI: 10.1038/s41598-017-12135-7
|
|
7486
Okita N, Honda Y, Kishimoto N, Liao W, Azumi E, Hashimoto Y, Matsumoto N.
Supplementation of strontium to a chondrogenic medium promotes chondrogenic differentiation of human dedifferentiated fat cells.
Tissue Eng Part A
2015
21:1695-704
PubMed ID: 25669848
DOI: 10.1089/ten.TEA.2014.0282
|
|
14350
Purroy N, Abrisqueta P, Carabia J, Carpio C, Palacio C, Bosch F, Crespo M.
Co-culture of primary CLL cells with bone marrow mesenchymal cells, CD40 ligand and CpG ODN promotes proliferation of chemoresistant CLL cells phenotypically comparable to those proliferating in vivo
Oncotarget
2015
6(10):7632-43
PubMed ID: 25544766
DOI: 10.18632/oncotarget.2939
|
|
15075
Ono M, Ohkouchi S, Kanehira M, Tode N, Kobayashi M, Ebina M, Nukiwa T, Irokawa T, Ogawa H, Akaike T, Okada Y, Kurosawa H, Kikuchi T, Ichinose M.
Mesenchymal stem cells correct inappropriate epithelial-mesenchyme relation in pulmonary fibrosis using stanniocalcin-1
Mol Ther
2015
23(3):549-60
PubMed ID: 25373521
DOI: 10.1038/mt.2014.217
|
|
15157
Morikawa A, Takeuchi T, Kito Y, Saigo C, Sakuratani T, Futamura M, Yoshida K.
Expression of beclin-1 in the microenvironment of invasive ductal carcinoma of the breast: correlation with prognosis and the cancer-stromal interaction
PLoS One
2015
10(5):e0125762
PubMed ID: 25955408
DOI: 10.1371/journal.pone.0125762
|
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