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Cell No. : Cell Name
RCB2153 : UE6E7T-2  update : 2022/05/10
CommentHuman mesenchymal cell line derived from bone marrow. Immortalized by HPV E6, E7, and hTERT. RCB2152- RCB2163 are derived from the same bone marrow.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2005
Animal human < Mammals
Genus Homo
Species sapiens
Tissue bone marrow
Classification transformed
Recombinant recombinant
Exogene LXSN-E7, LXSH-hTERT, MSCVpuro-16E6SDD151
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3236
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives POWEREDBY10
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 5 x 10^(5) cells/100 mm dish
SC frequency Subculture or Medium Renewal : once/3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
STR(human) OK
Reference information Reference 3
User's Publication 6


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Reference
4026  Mori T, Kiyono T, Imabayashi H, Takeda Y, Tsuchiya K, Miyoshi S, Makino H, Matsumoto K, Saito H, Ogawa S, Sakamoto M, Hata J, Umezawa A.  Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.  Mol Cell Biol  2005  25:5183-5195  PubMed ID: 15923633  
4027  Takeda Y, Mori T, Imabayashi H, Kiyono T, Gojo S, Miyoshi S, Hida N, Ita M, Segawa K, Ogawa S, Sakamoto M, Nakamura S, Umezawa A.  Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation?  J Gene Med  2004  6:833-845  PubMed ID: 15293342  
4028  Imabayashi H, Mori T, Gojo S, Kiyono T, Sugiyama T, Irie R, Isogai T, Hata J, Toyama Y, Umezawa A.  Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis  Exp Cell Res  2003  288:35-50  PubMed ID: 12878157  

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User's Publication
9959  Purroy N, Carabia J, Abrisqueta P, Egia L, Aguiló M, Carpio C, Palacio C, Crespo M, Bosch F.  Inhibition of BCR signaling using the Syk inhibitor TAK-659 prevents stroma-mediated signaling in chronic lymphocytic leukemia cells.  Oncotarget  2017  8:742-756  PubMed ID: 27888629   DOI: 10.18632/oncotarget.13557
14750  Carabia J, Carpio C, Abrisqueta P, Jiménez I, Purroy N, Calpe E, Palacio C, Bosch F, Crespo M.  Microenvironment regulates the expression of miR-21 and tumor suppressor genes PTEN, PIAS3 and PDCD4 through ZAP-70 in chronic lymphocytic leukemia  Sci Rep  2017  7(1):12262  PubMed ID: 28947822   DOI: 10.1038/s41598-017-12135-7
7486  Okita N, Honda Y, Kishimoto N, Liao W, Azumi E, Hashimoto Y, Matsumoto N.  Supplementation of strontium to a chondrogenic medium promotes chondrogenic differentiation of human dedifferentiated fat cells.  Tissue Eng Part A  2015  21:1695-704  PubMed ID: 25669848   DOI: 10.1089/ten.TEA.2014.0282
14350  Purroy N, Abrisqueta P, Carabia J, Carpio C, Palacio C, Bosch F, Crespo M.  Co-culture of primary CLL cells with bone marrow mesenchymal cells, CD40 ligand and CpG ODN promotes proliferation of chemoresistant CLL cells phenotypically comparable to those proliferating in vivo  Oncotarget  2015  6(10):7632-43  PubMed ID: 25544766   DOI: 10.18632/oncotarget.2939
15075  Ono M, Ohkouchi S, Kanehira M, Tode N, Kobayashi M, Ebina M, Nukiwa T, Irokawa T, Ogawa H, Akaike T, Okada Y, Kurosawa H, Kikuchi T, Ichinose M.  Mesenchymal stem cells correct inappropriate epithelial-mesenchyme relation in pulmonary fibrosis using stanniocalcin-1  Mol Ther  2015  23(3):549-60  PubMed ID: 25373521   DOI: 10.1038/mt.2014.217
15157  Morikawa A, Takeuchi T, Kito Y, Saigo C, Sakuratani T, Futamura M, Yoshida K.  Expression of beclin-1 in the microenvironment of invasive ductal carcinoma of the breast: correlation with prognosis and the cancer-stromal interaction  PLoS One  2015  10(5):e0125762  PubMed ID: 25955408   DOI: 10.1371/journal.pone.0125762



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