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Cell No. : Cell Name
RCB2151 : ZKOP  update : 2021/05/20
CommentSubline of KOP cell line. Zeocin-resistant.
Comment from the depositor
Terms and conditions1) Research purpose only. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochem Biophys Res Commun 2005;337:415-421) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sudo, Tatsuhiko
Originator Sudo, Tatsuhiko
Year of deposit 2005
Animal mouse < Mammals
Gender Female
Tissue embryo
Classification mutant
Recombinant recombinant
Exogene neo, zeo
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_6756
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 0


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Reference
3454  Sudo, Tatsuhiko, Kawai, Kayoko, Matsuzaki, Hiroshi, Osada, Hiroyuki  p38 mitogen-activated protein kinase plays a key role in regulating MAPKAPK2 expression.  Biochem Biophys Res Commun  2005  337:415-21  PubMed ID: 16198317

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