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Cell No. : Cell Name
RCB2148 : KOP update : 2023/10/16 |
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| Comment | Mouse fibroblast-like cell line lacking p38 MAP kinase expression. | |||
| Comment from the depositor | ||||
| Terms and conditions | 1) Research purpose only. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochem Biophys Res Commun 2005;337:415-421) designated by the DEPOSITOR is required. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Sudo, Tatsuhiko | ||
| Originator | Sudo, Tatsuhiko | |||
| Year of deposit | 2005 | |||
| Animal | _mouse < Mammals | |||
| Gender | Female | |||
| Tissue | embryo | |||
| Classification | mutant | |||
| Recombinant | recombinant | |||
| Exogene | knockout of p38, neo | Lifespan | infinite | |
| Morphology | fibroblast-like | |||
| Cellosaurus(Expasy) | CVCL_6753 | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | MEM ALPHA + 10% FBS | |||
| Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 4 split |
| SC frequency | Subculture : once/week, Medium Renewal : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma | (-) | |||
| SSLP(mouse) | OK | |||
| Isozyme | LD, NP | |||
| Images | ||||
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| Reference information | Reference | 1 | ||
| User's Publication | 1 | |||
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