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Cell No. : Cell Name
RCB2146 : WERI-Rb-1  update : 2024/01/17
CommentHuman cell line derived from retinoblastoma. TKG0601 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Obinata, Masuo
Originator McFall R.M. & Sery T.W.
Year of deposit 2005
Original cell TKG0601
Animal _human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Female
Age at sampling 1 year
Tissue eye
Disease name retinoblastoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0601)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1792
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 2
User's Publication 26

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4982  McFall RC, Nagy RM, Nagle BT, McGreevy LM.  Scanning electron microscopic observation of two retinoblastoma cell lines.  Cancer Res  1978  38(9):2827-35  PubMed ID: 679190  
4981  McFall RC, Sery TW, Makadon M.  Characterization of a new continuous cell line derived from a human retinoblastoma.  Cancer Res  1977  37(4):1003-10  PubMed ID: 844036  

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User's Publication
21579  Khade OS, Sasidharan S, Jain A, Maradani BS, Chatterjee A, Gopal D, Ravi Kumar RK, Krishnakumar S, Pandey A, Janakiraman N, Elchuri SV, Gundimeda S.  Identification of dysregulation of sphingolipids in retinoblastoma using liquid chromatography-mass spectrometry.  Exp Eye Res  2024  109798  PubMed ID: 38246332   DOI: 10.1016/j.exer.2024.109798
18226  Maradani BS, Parameswaran S, Subramanian K.  Development and characterization of DNA aptamer against Retinoblastoma by Cell-SELEX  Sci Rep  2022  12(1):16178  PubMed ID: 36171412   DOI: 10.1038/s41598-022-20660-3
20647  Kakkassery V, Gemoll T, Kraemer MM, Sauer T, Tura A, Ranjbar M, Grisanti S, Joachim SC, Mergler S, Reinhard J.  Protein Profiling of WERI-RB1 and Etoposide-Resistant WERI-ETOR Reveals New Insights into Topoisomerase Inhibitor Resistance in Retinoblastoma.  Int J Mol Sci  2022  23(7)  PubMed ID: 35409416   DOI: 10.3390/ijms23074058
13082  Raguraman R, Parameswaran S, Kanwar JR, Vasudevan M, Chitipothu S, Kanwar RK, Krishnakumar S.  Gene expression profiling of tumor stroma interactions in retinoblastoma.  Exp Eye Res  2020    PubMed ID: 32585195   DOI: 10.1016/j.exer.2020.108067
13774  Ravishankar H, Mangani AS, Shankar MB, Joshi M, Devasena T, Parameswaran S, Subramaniam K.  Characterization of NCC-RbC-51, an RB cell line isolated from a metastatic site  Histochem Cell Biol  2020  153(2):101-109  PubMed ID: 31781967   DOI: 10.1007/s00418-019-01832-1
13939  Ravishankar H, Mangani AS, Phoebe Moses GL, Mani SP, Parameswaran S, Khetan V, Ganesan S, Krishnakumar S.  Serum exosomal miRNA as biomarkers for Retinoblastoma  Exp Eye Res  2020  108184  PubMed ID: 32795526   DOI: 10.1016/j.exer.2020.108184
12604  Athyala PK, Chitipothu S, Kanwar JR, Krishnakumar S, Narayanan J.  Synthesis of saporin-antibody conjugates for targeting EpCAM positive tumour cells  IET Nanobiotechnol  2019  13(1):90-99  PubMed ID: 30964044   DOI: 10.1049/iet-nbt.2018.5222
14637  Athyala PK, Kanwar JR, Chitipothu S, Kanwar RK, Krishnakumar S, Watson JP, Narayanan J.  Neocarzinostatin, Aptamer Conjugates for Targeting EpCAM-positive Tumor Cells  Anticancer Res  2017  37(7):3615-3629  PubMed ID: 28668853   DOI: 10.21873/anticanres.11732
9718  Jayashree B, Srimany A, Jayaraman S, Bhutra A, Janakiraman N, Chitipothu S, Krishnakumar S, Baddireddi LS, Elchuri S, Pradeep T.  Monitoring of changes in lipid profiles during PLK1 knockdown in cancer cells using DESI MS.  Anal Bioanal Chem  2016  408:5623-32  PubMed ID: 27277815   DOI: 10.1007/s00216-016-9665-y
9743  Nalini V, Deepa PR, Raguraman R, Khetan V, Reddy MA, Krishnakumar S.  Targeting HMGA2 in Retinoblastoma Cells in vitro Using the Aptamer Strategy.  Ocul Oncol Pathol  2016  2:262-269  PubMed ID: 27843907   DOI: 10.1159/000447300
10861    Targeting CD44, ABCG2 and CD133 markers using aptamers: in silico analysis of CD133 extracellular domain 2 and its aptamer  RSC Adv.  2016  6, 32115-32123    DOI: 10.1039/C5RA27072C
14143  John A, Umashankar V, Samdani A, Sangeetha M, Krishnakumar S, Deepa PR.  In Silico Structure Prediction of Human Fatty Acid Synthase-Dehydratase: A Plausible Model for Understanding Active Site Interactions  Bioinform Biol Insights  2016  10:143-54  PubMed ID: 27559295   DOI: 10.4137/BBI.S38317
14153  Subramanian N, Srimany A, Kanwar JR, Kanwar RK, Akilandeswari B, Rishi P, Khetan V, Vasudevan M, Pradeep T, Krishnakumar S.  Nucleolin-aptamer therapy in retinoblastoma: molecular changes and mass spectrometry-based imaging  Mol Ther Nucleic Acids  2016  5(8):e358  PubMed ID: 27574784   DOI: 10.1038/mtna.2016.70
7226  Subramanian N, Kanwar JR, Kanwar RK, Krishnakumar S.  Targeting Cancer Cells Using LNA-Modified Aptamer-siRNA Chimeras.  Nucleic Acid Ther  2015  25:317-22  PubMed ID: 26334953   DOI: 10.1089/nat.2015.0550
7342  Venkatesan N, Deepa PR, Khetan V, Krishnakumar S.  Computational and in vitro Investigation of miRNA-Gene Regulations in Retinoblastoma Pathogenesis: miRNA Mimics Strategy.  Bioinform Biol Insights  2015  9:89-101  PubMed ID: 25983556   DOI: 10.4137/BBI.S21742
7360  Sangeetha M, Deepa PR, Rishi P, Khetan V, Krishnakumar S.  Global gene deregulations in FASN silenced retinoblastoma cancer cells: molecular and clinico-pathological correlations.  J. Cell. Biochem.  2015  116:2676-94  PubMed ID: 25958981   DOI: 10.1002/jcb.25217
7515  Subramanian N, Kanwar JR, Athalya PK, Janakiraman N, Khetan V, Kanwar RK, Eluchuri S, Krishnakumar S.  EpCAM aptamer mediated cancer cell specific delivery of EpCAM siRNA using polymeric nanocomplex.  J. Biomed. Sci.  2015  22:4  PubMed ID: 25576037   DOI: 10.1186/s12929-014-0108-9
9419  Tatsuya Yunoki, Yoshiaki Tabuchi, Atsushi Hayashi, Takashi Kondo  BAG3 Protects Against Hyperthermic Stress by Modulating NF-κB and ERK Activities in Human Retinoblastoma Cells  Graefes Arch Clin Exp Ophthalmol  2015  253(3):399-407  PubMed ID: 25471019   DOI: 10.1007/s00417-014-2874-1
14313  Subramanian N, Kanwar JR, Kanwar RK, Krishnakumar S.  Blocking the maturation of OncomiRNAs using pri-miRNA-17∼92 aptamer in retinoblastoma  Nucleic Acid Ther  2015  25(1):47-52  PubMed ID: 25513843   DOI: 10.1089/nat.2014.0507
14389  Sreenivasan S, Krishnakumar S.  Synergistic Effect of Curcumin in Combination with Anticancer Agents in Human Retinoblastoma Cancer Cell Lines  Curr Eye Res  2015  40(11):1153-65  PubMed ID: 25495096   DOI: 10.3109/02713683.2014.987870
14986  Subramanian N, Kanwar JR, Kanwar RK, Sreemanthula J, Biswas J, Khetan V, Krishnakumar S.  EpCAM Aptamer-siRNA Chimera Targets and Regress Epithelial Cancer  PLoS One  2015  10(7):e0132407  PubMed ID: 26176230   DOI: 10.1371/journal.pone.0132407
15237  Srimany A, Jayashree B, Krishnakumar S, Elchuri S, Pradeep T.  Identification of effective substrates for the direct analysis of lipids from cell lines using desorption electrospray ionization mass spectrometry  Rapid Commun Mass Spectrom  2015  29(4):349-56  PubMed ID: 26406347   DOI: 10.1002/rcm.7111
13761  Venkatesan N, Krishnakumar S, Deepa PR, Deepa M, Khetan V, Reddy MA.  Molecular deregulation induced by silencing of the high mobility group protein A2 gene in retinoblastoma cells  Mol Vis  2012  18:2420-37  PubMed ID: 23077401  
8007  Endo S, Yamato K, Hirai S, Moriwaki T, Fukuda K, Suzuki H, Abei M, Nakagawa I, Hyodo I.  Potent in vitro and in vivo antitumor effects of MDM2 inhibitor nutlin-3 in gastric cancer cells.  Cancer Sci.  2011  102:605-13  PubMed ID: 21205074   DOI: 10.1111/j.1349-7006.2010.01821.x
19029  Kanamori-Katayama M, Kaiho A, Ishizu Y, Okamura-Oho Y, Hino O, Abe M, Kishimoto T, Sekihara H, Nakamura Y, Suzuki H, Forrest AR, Hayashizaki Y.  LRRN4 and UPK3B are markers of primary mesothelial cells  PLoS One  2011  6(10):e25391  PubMed ID: 21984916   DOI: 10.1371/journal.pone.0025391
8620  Mikami-Terao Y, Akiyama M, Yuza Y, Yanagisawa T, Yamada O, Kawano T, Agawa M, Ida H, Yamada H.  Antitumor activity of TMPyP4 interacting G-quadruplex in retinoblastoma cell lines.  Exp. Eye Res.  2009  89:200-8  PubMed ID: 19328784   DOI: 10.1016/j.exer.2009.03.008

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