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Cell No. : Cell Name
RCB2145 : PC-3  update : 2022/04/08
CommentHuman cell line derived from bone metastasis of prostatic cancer. TKG0600 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Obinata, Masuo
Year of deposit 2005
Original cell TKG0600
Animal human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Male
Age at sampling 62 years
Tissue prostate, bone meta
Disease name prostatic carcinom
Metastatic ability Yes
Metastatic tissue bone
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0600)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0035
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 4
User's Publication 52

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5121  Kirk D, Szalay MF, Kaighn ME.  Modulation of growth of a human prostatic cancer cell line (PC-3) in agar culture by normal human lung fibroblasts.  Cancer Res  1981  41(3):1100-3  PubMed ID: 7459854
5120  Ohnuki Y, Marnell MM, Babcock MS, Lechner JF, Kaighn ME.  Chromosomal analysis of human prostatic adenocarcinoma cell lines.  Cancer Res  1980  40(3):524-34  PubMed ID: 7471073
5118  Kaighn ME, Narayan KS, Ohnuki Y, Lechner JF, Jones LW.  Establishment and characterization of a human prostatic carcinoma cell line (PC-3).  Invest Urol  1979  17(1):16-23  PubMed ID: 447482
5119  Kaighn ME, Lechner JF, Narayan KS, Jones LW.  Prostate carcinoma: tissue culture cell lines.  Natl Cancer Inst Monogr  1978  (49):17-21  PubMed ID: 571045

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User's Publication
20582  Qian Y, Liu X, Feng Y, Li X, Xuan Y.  Tenascin C regulates cancer cell glycolysis and tumor progression in prostate cancer.  Int J Urol  2022    PubMed ID: 35218089
20668  Mitani F, Lin J, Sakamoto T, Uehara R, Hikita T, Yoshida T, Setiawan A, Arai M, Oneyama C.  Asteltoxin inhibits extracellular vesicle production through AMPK/mTOR-mediated activation of lysosome function.  Sci Rep  2022  12(1):6674  PubMed ID: 35461323
13130  Sasaki K, Harada M, Yoshikawa T, Tagawa H, Harada Y, Yonemitsu Y, Ryujin T, Kishimura A, Mori T, Katayama Y.  Fc-Binding Antibody-Recruiting Molecules Targeting Prostate-Specific Membrane Antigen: Defucosylation of Antibody for Efficacy Improvement*.  Chembiochem  2021  22(3):496-500  PubMed ID: 32969164
13395  Imamura R, Kitagawa S, Kubo T, Irie A, Kariu T, Yoneda M, Kamba T, Imamura T.  Prostate cancer C5a receptor expression and augmentation of cancer cell proliferation, invasion, and PD-L1 expression by C5a.  Prostate  2021    PubMed ID: 33368414
20153  Yin X, Yamada S, Kobayashi H, Tanaka R, Togo Y, Hosoi M, Tsuchida M, Kunoh T, Wada S, Nakamura T, Sasaki R, Mizukami T, Hasegawa M.  Expression and cell transformation activity of dynactin-associated protein isoforms.  FEBS Open Bio  2021    PubMed ID: 34043884
20249  Makise M, Uchimura R, Higashi K, Mashiki Y, Shiraishi R, Shutoku Y, Kuniyasu A.  Overexpression of the nucleoporin Nup88 stimulates migration and invasion of HeLa cells.  Histochem Cell Biol  2021    PubMed ID: 34331103
9833  Keiichi Oono, Kazuo Ohtake, Chie Watanabe, Sachiko Shiba, Takashi Sekiya, Keizo Kasono  Contribution of Pyk2 Pathway and Reactive Oxygen Species (ROS) to the Anti-Cancer Effects of Eicosapentaenoic Acid (EPA) in PC3 Prostate Cancer Cells  Lipids Health Dis  2020  19(1):15  PubMed ID: 32005121
12088  Nagasawa M, Tomimatsu K, Terada K, Kondo K, Miyazaki K, Miyazaki M, Motooka D, Okuzaki D, Yoshida T, Kageyama S, Kawamoto H, Kawauchi A, Agata Y.  Long non-coding RNA MANCR is a target of BET bromodomain protein BRD4 and plays a critical role in cellular migration and invasion abilities of prostate cancer.  Biochem. Biophys. Res. Commun.  2020    PubMed ID: 32199616
12152  Shogo Nomura, Yasuko Egawa, Sayaka Urano, Tsuyoshi Tahara, Yasuyoshi Watanabe & Katsunori Tanaka  Cancer discrimination by on-cell N-glycan ligation  Communications Chemistry  2020   
10510  Kenward Vong, Tomoya Yamamoto, Tsung-che Changa and Katsunori Tanaka   Bioorthogonal release of anticancer drugs via gold-triggered 2-alkynylbenzamide cyclization  Chemical Science  2020  11:10928-10933 
13198  Hiromi I, Nakata S, Uenishi J.  Up-regulation of Death Receptor 5/TRAIL-R2 Mediates Apoptosis Induced by N,N'-[(3,4-dimethoxyphenyl)methylene] Biscinnamide in Cancer Cells.  Anticancer Res  2020    PubMed ID: 32878791
13264  Hikita T, Miyata M, Watanabe R, Oneyama C.  In vivo imaging of long-term accumulation of cancer-derived exosomes using a BRET-based reporter.  Sci Rep  2020    PubMed ID: 33024173
13303  Uwada J, Nakazawa H, Mikami D, Islam MS, Muramatsu I, Taniguchi T, Yazawa T.  PNU-120596, a positive allosteric modulator of α7 nicotinic acetylcholine receptor, directly inhibits p38 MAPK.  Biochem Pharmacol  2020    PubMed ID: 33132165
13317  Fujita K, Kamiya M, Yoshioka T, Ogasawara A, Hino R, Kojima R, Ueo H, Urano Y.  Rapid and Accurate Visualization of Breast Tumors with a Fluorescent Probe Targeting α-Mannosidase 2C1.  ACS Cent Sci  2020    PubMed ID: 33376783
13591  Saiki I, Yara M, Yamanaka T, Uchino H, Inazu M.  Functional Expression of Choline Transporter-Like Protein 1 in LNCaP Prostate Cancer Cells: A Novel Molecular Target  Biomol Ther (Seoul)  2020  28(2):195-201  PubMed ID: 31693854
13771  F. Sima, H. Kawano, A. Miyawaki, K. Obata, D. Serien, K. Sugioka  3D glass nanofluidics fabricated by femtosecond laser processing for study of cancer cell metastasis and invasion  SPIE LASE  2020   
11271  Itoh A, Nonaka Y, Ogawa T, Nakamura T, Nishi N.  Galectin-9 induces atypical ubiquitination leading to cell death in PC-3 prostate cancer cells.  Glycobiology  2019    PubMed ID: 30383222
11464  Ishigami-Yuasa M, Ekimoto H, Kagechika H.  Class IIb HDAC Inhibition Enhances the Inhibitory Effect of Am80, a Synthetic Retinoid, in Prostate Cancer.  Biol. Pharm. Bull.  2019    PubMed ID: 30828077
11507  Murata K, Saga R, Monzen S, Tsuruga E, Hasegawa K, Hosokawa Y.  Understanding the mechanism underlying the acquisition of radioresistance in human prostate cancer cells.  Oncol Lett  2019    PubMed ID: 31186811
11639  Taniguchi K, Ii H, Kageyama S, Takagi H, Chano T, Kawauchi A, Nakata S.  Depletion of gamma-glutamylcyclotransferase inhibits cancer cell growth by activating the AMPK-FOXO3a-p21 axis.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 31345573
11800  Koizumi A, Narita S, Nakanishi H, Ishikawa M, Eguchi S, Kimura H, Takasuga S, Huang M, Inoue T, Sasaki J, Yoshioka T, Habuchi T, Sasaki T.  Increased fatty acyl saturation of phosphatidylinositol phosphates in prostate cancer progression.  Sci Rep  2019    PubMed ID: 31520002
11807  Takagi H, Ii H, Kageyama S, Hanada E, Taniguchi K, Yoshiya T, Chano T, Kawauchi A, Nakata S.  Blockade of γ-Glutamylcyclotransferase Enhances Docetaxel Growth Inhibition of Prostate Cancer Cells.  Anticancer Res.  2019    PubMed ID: 31519583
11717  K Hayashi et al  Optimization of Surface Treatment of MEMS Probes for Single-cell Capture and Release  Sensors and Materials  2019  31(9):2873 
13859  Park J, Komori T, Uda T, Miyajima K, Fujii T, Kim SH.  Sequential Cell-Processing System by Integrating Hydrodynamic Purification and Dielectrophoretic Trapping for Analyses of Suspended Cancer Cells  Micromachines (Basel)  2019  11(1):47  PubMed ID: 31905986
10845  Taniguchi K, Matsumura K, Ii H, Kageyama S, Ashihara E, Chano T, Kawauchi A, Yoshiki T, Nakata S.  Depletion of gamma-glutamylcyclotransferase in cancer cells induces autophagy followed by cellular senescence.  Am J Cancer Res  2018  8:650-661  PubMed ID: 29736310
11105  Senga S, Kobayashi N, Kawaguchi K, Ando A, Fujii H.  Fatty acid-binding protein 5 (FABP5) promotes lipolysis of lipid droplets, de novo fatty acid (FA) synthesis and activation of nuclear factor-kappa B (NF-κB) signaling in cancer cells.  Biochim Biophys Acta Mol Cell Biol Lipids  2018  1863(9):1057-1067  PubMed ID: 29906613
11160  Languon S, Tuffour I, Quayson EE, Appiah-Opong R, Quaye O.  In Vitro Evaluation of Cytotoxic Activities of Marketed Herbal Products in Ghana.  J Evid Based Integr Med  2018    PubMed ID: 30088418
4319  Liu Q, Wu Y, Seino H, Haga T, Yoshizawa T, Morohashi S, Kijima H.  Correlation between DEC1/DEC2 and epithelial‑mesenchymal transition in human prostate cancer PC‑3 cells.  Mol Med Rep  2018    PubMed ID: 30106153
14599  Senga S, Kawaguchi K, Kobayashi N, Ando A, Fujii H.  A novel fatty acid-binding protein 5-estrogen-related receptor α signaling pathway promotes cell growth and energy metabolism in prostate cancer cells  Oncotarget  2018  9(60):31753-31770  PubMed ID: 30167092
7050  Kajiura K, Masuda K, Naruto T, Kohmoto T, Watabnabe M, Tsuboi M, Takizawa H, Kondo K, Tangoku A, Imoto I.  Frequent silencing of the candidate tumor suppressor TRIM58 by promoter methylation in early-stage lung adenocarcinoma.  Oncotarget  2017  8:2890-2905  PubMed ID: 27926516
10506  Doi H, Kida T, Nishino K, Nakatsuji M, Sakamoto S, Shimizu S, Teraoka Y, Tamura Y, Kataoka Y, Inui T.  Solubility-Improved 10-O-Substituted SN-38 Derivatives with Antitumor Activity.  ChemMedChem  2017  12:1715-1722  PubMed ID: 28891271
10973    Dynamics of Morphological Changes of Adherent Cultured Cells at a Range of Low Temperatures near Physiological Temperature (Time-Lapse Imaging by Digital Holographic Microscopy)  Cryobiology and Cryotechnology  2017  63, 45~51 
4534  Cui C, Hanyu M, Hatori A, Zhang Y, Xie L, Ohya T, Fukada M, Suzuki H, Nagatsu K, Jiang C, Luo R, Shao G, Zhang M, Wang F.  Synthesis and evaluation of [64Cu]PSMA-617 targeted for prostate-specific membrane antigen in prostate cancer.  Am J Nucl Med Mol Imaging  2017  7(2):40-52  PubMed ID: 28533936
14574  Shaoyi Chen, Stelios Florinas, Abigail Teitgen, Ze-Qi Xu, Changshou Gao, Herren Wu, Kazunori Kataoka, Horacio Cabral, R. James Christie  Controlled Fab installation onto polymeric micelle nanoparticles for tuned bioactivity  Science and Technology of Advanced Materials  2017  18:666-680 
9615  Ohya S, Kanatsuka S, Hatano N, Kito H, Matsui A, Fujimoto M, Matsuba S, Niwa S, Zhan P, Suzuki T, Muraki K.  Downregulation of the Ca(2+)-activated K(+) channel KC a3.1 by histone deacetylase inhibition in human breast cancer cells.  Pharmacol Res Perspect  2016  4:e00228  PubMed ID: 27069638
9862  Sakura Y, Tsuboi K, Uyama T, Zhang X, Taoka R, Sugimoto M, Kakehi Y, Ueda N.  A quantitative study on splice variants of N-acylethanolamine acid amidase in human prostate cancer cells and other cells.  Biochim. Biophys. Acta  2016  1861:1951-1958  PubMed ID: 27693242
10024  Matsumura K, Nakata S, Taniguchi K, Ii H, Ashihara E, Kageyama S, Kawauchi A, Yoshiki T.  Depletion of γ-glutamylcyclotransferase inhibits breast cancer cell growth via cellular senescence induction mediated by CDK inhibitor upregulation.  BMC Cancer  2016  16:748  PubMed ID: 27658708
10908    Extracts of Codiaeum variegatum (L.) A. Juss is Cytotoxic on Human Leukemic, Breast and Prostate Cancer Cell Lines  Journal of Applied Pharmaceutical Science  2016  6, 087-093 
6034  Liu Q, Wu Y, Yoshizawa T, Yan X, Morohashi S, Seino H, Kato Y, Kijima H.  Basic helix-loop-helix transcription factor DEC2 functions as an anti-apoptotic factor during paclitaxel-induced apoptosis in human prostate cancer cells.  Int J Mol Med  2016  38(6):1727-1733  PubMed ID: 27840924
12610  Zhang B, Shimada Y, Hirota T, Ariyoshi M, Kuroyanagi J, Nishimura Y, Tanaka T.  Novel immunologic tolerance of human cancer cell xenotransplants in zebrafish  Transl Res  2016  170:89-98.e3  PubMed ID: 26746804
12621  Okada T, Uto K, Aoyagi T, Ebara M.  A biomimetic approach to hormone resistant prostate cancer cell isolation using inactivated Sendai virus (HVJ-E)  Biomater Sci  2016  4(1):96-103  PubMed ID: 26553555
12660  Thangavel S, Yoshitomi T, Sakharkar MK, Nagasaki Y.  Redox nanoparticle increases the chemotherapeutic efficiency of pioglitazone and suppresses its toxic side effects  Biomaterials  2016  99:109-23  PubMed ID: 27235996
12452  Kim SH, Fujii T.  Efficient analysis of a small number of cancer cells at the single-cell level using an electroactive double-well array  Lab Chip  2016  16(13):2440-9  PubMed ID: 27189335
14141  Nara T, Narita S, Mingguo H, Yoshioka T, Koizumi A, Numakura K, Tsuruta H, Maeno A, Saito M, Inoue T, Tsuchiya N, Satoh S, Habuchi T.  Altered miRNA expression in high-fat diet-induced prostate cancer progression  Carcinogenesis  2016  37(12):1129-1137  PubMed ID: 27915273
7447  Sakane C, Ohta H, Shidoji Y.  Measurement of lysine-specific demethylase-1 activity in the nuclear extracts by flow-injection based time-of-flight mass spectrometry.  J Clin Biochem Nutr  2015  56:123-31  PubMed ID: 25759518
12866  Okada T, Niiyama E, Uto K, Aoyagi T, Ebara M.  Inactivated Sendai Virus (HVJ-E) Immobilized Electrospun Nanofiber for Cancer Therapy  Materials (Basel)  2015  9(1):12  PubMed ID: 28787810
14955  Nishikawa Y, Okuzaki D, Fukushima K, Mukai S, Ohno S, Ozaki Y, Yabuta N, Nojima H.  Withaferin A Induces Cell Death Selectively in Androgen-Independent Prostate Cancer Cells but Not in Normal Fibroblast Cells  PLoS One  2015  10(7):e0134137  PubMed ID: 26230090
15159  Honda S, Loher P, Shigematsu M, Palazzo JP, Suzuki R, Imoto I, Rigoutsos I, Kirino Y.  Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers  Proc Natl Acad Sci U S A  2015  112(29):E3816-25  PubMed ID: 26124144
15240  Kobayashi M, Kim SH, Nakamura H, Kaneda S, Fujii T.  Cancer Cell Analyses at the Single Cell-Level Using Electroactive Microwell Array Device  PLoS One  2015  10(11):e0139980  PubMed ID: 26558904
16006  Ramesh V, Ananda Rao B, Sharma P, Swarna B, Thummuri D, Srinivas K, Naidu VG, Jayathirtha Rao V.  Synthesis and biological evaluation of new rhodanine analogues bearing 2-chloroquinoline and benzo[h]quinoline scaffolds as anticancer agents  Eur J Med Chem  2014  83:569-80  PubMed ID: 24996143
16648  Konishi J, Yamazaki K, Yokouchi H, Shinagawa N, Iwabuchi K, Nishimura M.  The characteristics of human NKT cells in lung cancer--CD1d independent cytotoxicity against lung cancer cells by NKT cells and decreased human NKT cell response in lung cancer patients  Hum Immunol  2004  65(11):1377-88  PubMed ID: 15556688
5547  Orui H, Imaizumi S, Ogino T, Motoyama T.  Effects of bone morphogenetic protein-2 on human tumor cell growth and differentiation: a preliminary report.  J Orthop Sci  2000  5(6):600-4  PubMed ID: 11180925

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