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Cell No. : Cell Name
RCB2144 : LNCap.FGC  update : 2021/02/26
CommentHuman cell line derived from prostate adenocarcinoma. Derived from lymph node metastasis. TKG0603 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Obinata, Masuo
Originator Horoszewicz J.S.
Year of deposit 2005
Original cell TKG0603
Animal _human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Male
Age at sampling 50 years
Tissue prostate, lymph node meta
Disease name prostaric carcinoma
Metastastatic ability Yes
Metastatsd tissue lymph node
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0603)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 4
User's Publication 36

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5117  Deguchi T, Chu TM, Leong SS, Horoszewicz JS, Lee CL.  Effect of methotrexate-monoclonal anti-prostatic acid phosphatase antibody conjugate on human prostate tumor.  Cancer Res  1986  46(8):3751-5  PubMed ID: 3731053
5115  Gibas Z, Becher R, Kawinski E, Horoszewicz J, Sandberg AA.  A high-resolution study of chromosome changes in a human prostatic carcinoma cell line (LNCaP).  Cancer Genet Cytogenet  1984  11(4):399-404  PubMed ID: 6584201
5114  Horoszewicz JS, Leong SS, Kawinski E, Karr JP, Rosenthal H, Chu TM, Mirand EA, Murphy GP.  LNCaP model of human prostatic carcinoma.  Cancer Res  1983  3(4):1809-18  PubMed ID: 6831420
5116  Horoszewicz JS, Leong SS, Chu TM, Wajsman ZL, Friedman M, Papsidero L, Kim U, Chai LS, Kakati S, Arya SK, Sandberg AA.  The LNCaP cell line--a new model for studies on human prostatic carcinoma.  Prog Clin Biol Res  1980  37:115-32  PubMed ID: 384082

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User's Publication
13393  Miyasaka K, Takeda S, Nakamura S, Matsuda H, Shimoda H.  Estrogenic and antiandrogenic activities of methoxyflavans isolated from the fruit of Mauritia Flexuosa (Moriche Palm).  J Food Biochem  2021    PubMed ID: 33340139
12056  Ideo H, Kondo J, Nomura T, Nonomura N, Inoue M, Amano J.  Study of glycosylation of prostate-specific antigen secreted by cancer tissue-originated spheroids reveals new candidates for prostate cancer detection.  Sci Rep  2020    PubMed ID: 32066783
13130  Sasaki K, Harada M, Yoshikawa T, Tagawa H, Harada Y, Yonemitsu Y, Ryujin T, Kishimura A, Mori T, Katayama Y.  Fc-Binding Antibody-Recruiting Molecules Targeting Prostate-Specific Membrane Antigen: Defucosylation of Antibody for Efficacy Improvement*.  Chembiochem  2020    PubMed ID: 32969164
13264  Hikita T, Miyata M, Watanabe R, Oneyama C.  In vivo imaging of long-term accumulation of cancer-derived exosomes using a BRET-based reporter.  Sci Rep  2020    PubMed ID: 33024173
13317  Fujita K, Kamiya M, Yoshioka T, Ogasawara A, Hino R, Kojima R, Ueo H, Urano Y.  Rapid and Accurate Visualization of Breast Tumors with a Fluorescent Probe Targeting α-Mannosidase 2C1.  ACS Cent Sci  2020    PubMed ID: 33376783
13591  Saiki I, Yara M, Yamanaka T, Uchino H, Inazu M.  Functional Expression of Choline Transporter-Like Protein 1 in LNCaP Prostate Cancer Cells: A Novel Molecular Target  Biomol Ther (Seoul)  2020  28(2):195-201  PubMed ID: 31693854
11446  Wada A, Terashima T, Kageyama S, Yoshida T, Narita M, Kawauchi A, Kojima H.  Efficient Prostate Cancer Therapy with Tissue-Specific Homing Peptides Identified by Advanced Phage Display Technology.  Mol Ther Oncolytics  2019    PubMed ID: 30788426
11507  Murata K, Saga R, Monzen S, Tsuruga E, Hasegawa K, Hosokawa Y.  Understanding the mechanism underlying the acquisition of radioresistance in human prostate cancer cells.  Oncol Lett  2019    PubMed ID: 31186811
11533  Saga R, Matsuya Y, Takahashi R, Hasegawa K, Date H, Hosokawa Y.  Analysis of the high-dose-range radioresistance of prostate cancer cells, including cancer stem cells, based on a stochastic model.  J. Radiat. Res.  2019    PubMed ID: 31034058
11591  Kawatani M, Yamamoto K, Yamada D, Kamiya M, Miyakawa J, Miyama Y, Kojima R, Morikawa T, Kume H, Urano Y.  Fluorescence Detection of Prostate Cancer by an Activatable Fluorescence Probe for PSMA Carboxypeptidase Activity.  J. Am. Chem. Soc.  2019    PubMed ID: 31244179
11800  Koizumi A, Narita S, Nakanishi H, Ishikawa M, Eguchi S, Kimura H, Takasuga S, Huang M, Inoue T, Sasaki J, Yoshioka T, Habuchi T, Sasaki T.  Increased fatty acyl saturation of phosphatidylinositol phosphates in prostate cancer progression.  Sci Rep  2019    PubMed ID: 31520002
11807  Takagi H, Ii H, Kageyama S, Hanada E, Taniguchi K, Yoshiya T, Chano T, Kawauchi A, Nakata S.  Blockade of γ-Glutamylcyclotransferase Enhances Docetaxel Growth Inhibition of Prostate Cancer Cells.  Anticancer Res.  2019    PubMed ID: 31519583
6922  Soeda F, Watabe T, Naka S, Liu Y, Horitsugi G, Neels OC, Kopka K, Tatsumi M, Shimosegawa E, Giesel FL, Hatazawa J.  Impact of 18F-PSMA-1007 Uptake in Prostate Cancer Using Different Peptide Concentrations: Preclinical PET/CT Study on Mice.  J Nucl Med  2019  60(11):1594-1599  PubMed ID: 30902876
12506  Jin C, Zhao W, Zhang Z, Liu W.  Silencing circular RNA circZNF609 restrains growth, migration and invasion by up-regulating microRNA-186-5p in prostate cancer  Artif Cells Nanomed Biotechnol  2019  47(1):3350-3358  PubMed ID: 31387394
11152  Kamei H, Noguchi K, Matsuda H, Murata K.  Screening of Euphorbiaceae Plant Extracts for Anti-5α-reductase.  Biol. Pharm. Bull.  2018  41(8):1307-1310  PubMed ID: 30068884
11156  Ito S, Kayukawa N, Ueda T, Taniguchi H, Morioka Y, Hongo F, Ukimura O.  MRGBP promotes AR-mediated transactivation of KLK3 and TMPRSS2 via acetylation of histone H2A.Z in prostate cancer cells.  Biochim Biophys Acta Gene Regul Mech  2018    PubMed ID: 30076933
11160  Languon S, Tuffour I, Quayson EE, Appiah-Opong R, Quaye O.  In Vitro Evaluation of Cytotoxic Activities of Marketed Herbal Products in Ghana.  J Evid Based Integr Med  2018    PubMed ID: 30088418
4279  Ito S, Ueno A, Ueda T, Nakagawa H, Taniguchi H, Kayukawa N, Fujihara-Iwata A, Hongo F, Okihara K, Ukimura O.  CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells.  Oncotarget  2018    PubMed ID: 29707137
10057  Li X, Wang X, Zhang J, Hanagata N, Wang X, Weng Q, Ito A, Bando Y, Golberg D.  Hollow boron nitride nanospheres as boron reservoir for prostate cancer treatment.  Nat Commun  2017  8:13936  PubMed ID: 28059072
10403  Qin H, Rasul A, Li X, Masood M, Yang G, Wang N, Wei W, He X, Watanabe N, Li J, Li X.  CD147-induced cell proliferation is associated with Smad4 signal inhibition.  Exp. Cell Res.  2017  358:279-289  PubMed ID: 28684116
10514  Kanayama M, Hayano T, Koebis M, Maeda T, Tabe Y, Horie S, Aiba A.  Hyperactive mTOR induces neuroendocrine differentiation in prostate cancer cell with concurrent up-regulation of IRF1.  Prostate  2017  77:1489-1498  PubMed ID: 28905415
4534  Cui C, Hanyu M, Hatori A, Zhang Y, Xie L, Ohya T, Fukada M, Suzuki H, Nagatsu K, Jiang C, Luo R, Shao G, Zhang M, Wang F.  Synthesis and evaluation of [64Cu]PSMA-617 targeted for prostate-specific membrane antigen in prostate cancer.  Am J Nucl Med Mol Imaging  2017  7(2):40-52  PubMed ID: 28533936
9650  Ogura T, Tanaka Y, Tamaki H, Harada M.  Docetaxel induces Bcl-2- and pro-apoptotic caspase-independent death of human prostate cancer DU145 cells.  Int. J. Oncol.  2016  48:2330-8  PubMed ID: 27082738
9862  Sakura Y, Tsuboi K, Uyama T, Zhang X, Taoka R, Sugimoto M, Kakehi Y, Ueda N.  A quantitative study on splice variants of N-acylethanolamine acid amidase in human prostate cancer cells and other cells.  Biochim. Biophys. Acta  2016  1861:1951-1958  PubMed ID: 27693242
9868  Ito H, Yamaguchi N, Onimaru M, Kimura S, Ohmori T, Ishikawa F, Sato J, Ito S, Inoue H.  Change in number and size of circulating tumor cells with high telomerase activity during treatment of patients with gastric cancer.  Oncol Lett  2016  12:4720-4726  PubMed ID: 28105179
10024  Matsumura K, Nakata S, Taniguchi K, Ii H, Ashihara E, Kageyama S, Kawauchi A, Yoshiki T.  Depletion of γ-glutamylcyclotransferase inhibits breast cancer cell growth via cellular senescence induction mediated by CDK inhibitor upregulation.  BMC Cancer  2016  16:748  PubMed ID: 27658708
10905    Roasted coffee induction of aldo-keto reductase 1C3 expression in LNCaP human prostate cancer cells is associated with Nrf2 activation  Oncology Letters  2016  12, 5321-5326 
12204  Minhui Xu, Shinichi Sakamoto, Jun Matsushima, Toru Kimura, Takeshi Ueda, Atsushi Mizokami, Yoshikatsu Kanai, Tomohiko Ichikawa  Up-Regulation of LAT1 during Antiandrogen Therapy Contributes to Progression in Prostate Cancer Cells  J Urol  2016  195(5):1588-1597  PubMed ID: 26682754
12621  Okada T, Uto K, Aoyagi T, Ebara M.  A biomimetic approach to hormone resistant prostate cancer cell isolation using inactivated Sendai virus (HVJ-E)  Biomater Sci  2016  4(1):96-103  PubMed ID: 26553555
12637  Kawaguchi K, Kinameri A, Suzuki S, Senga S, Ke Y, Fujii H.  The cancer-promoting gene fatty acid-binding protein 5 (FABP5) is epigenetically regulated during human prostate carcinogenesis  Biochem J  2016  473(4):449-61  PubMed ID: 26614767
12660  Thangavel S, Yoshitomi T, Sakharkar MK, Nagasaki Y.  Redox nanoparticle increases the chemotherapeutic efficiency of pioglitazone and suppresses its toxic side effects  Biomaterials  2016  99:109-23  PubMed ID: 27235996
12683  Matsushita S, Suzuki K, Ogino Y, Hino S, Sato T, Suyama M, Matsumoto T, Omori A, Inoue S, Yamada G.  Androgen Regulates Mafb Expression Through its 3'UTR During Mouse Urethral Masculinization  Endocrinology  2016  157(2):844-57.  PubMed ID: 26636186
6398  Nagano K, Yamashita T, Inoue M, Higashisaka K, Yoshioka Y, Abe Y, Mukai Y, Kamada H, Tsutsumi Y, Tsunoda S  Eph receptor A10 has a potential as a target for a prostate cancer therapy.  Biochem Biophys Res Commun  2014  450(1):545-9  PubMed ID: 24924629
7747  Qin XY, Zaha H, Nagano R, Yoshinaga J, Yonemoto J, Sone H.  Xenoestrogens down-regulate aryl-hydrocarbon receptor nuclear translocator 2 mRNA expression in human breast cancer cells via an estrogen receptor alpha-dependent mechanism.  Toxicol. Lett.  2011  206:152-7  PubMed ID: 21771643
8419  Fukushima K, Satoh T, Baba S, Yamashita K.  alpha1,2-Fucosylated and beta-N-acetylgalactosaminylated prostate-specific antigen as an efficient marker of prostatic cancer.  Glycobiology  2010  20:452-60  PubMed ID: 20008118
10094  Fischer P, Kipp W, Bamuhiga J, Binta-Kahwa J, Kiefer A, Büttner DW.  Parasitological and clinical characterization of Simulium neavei-transmitted onchocerciasis in western Uganda.  Trop. Med. Parasitol.  1993  44:311-21  PubMed ID: 8134773

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