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Cell No. : Cell Name
RCB2144 : LNCap.FGC  update : 2022/11/04
CommentHuman cell line derived from prostate adenocarcinoma. Derived from lymph node metastasis. TKG0603 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Horoszewicz J.S.
Year of deposit 2005
Original cell TKG0603
Animal human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Male
Age at sampling 50 years
Tissue prostate, lymph node meta
Disease name prostaric carcinoma
Metastastatic ability Yes
Metastatsd tissue lymph node
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0603)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_1379
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 4
User's Publication 54


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Reference
5117  Deguchi T, Chu TM, Leong SS, Horoszewicz JS, Lee CL.  Effect of methotrexate-monoclonal anti-prostatic acid phosphatase antibody conjugate on human prostate tumor.  Cancer Res  1986  46(8):3751-5  PubMed ID: 3731053  
5115  Gibas Z, Becher R, Kawinski E, Horoszewicz J, Sandberg AA.  A high-resolution study of chromosome changes in a human prostatic carcinoma cell line (LNCaP).  Cancer Genet Cytogenet  1984  11(4):399-404  PubMed ID: 6584201   DOI: 10.1016/0165-4608(84)90020-7
5114  Horoszewicz JS, Leong SS, Kawinski E, Karr JP, Rosenthal H, Chu TM, Mirand EA, Murphy GP.  LNCaP model of human prostatic carcinoma.  Cancer Res  1983  3(4):1809-18  PubMed ID: 6831420  
5116  Horoszewicz JS, Leong SS, Chu TM, Wajsman ZL, Friedman M, Papsidero L, Kim U, Chai LS, Kakati S, Arya SK, Sandberg AA.  The LNCaP cell line--a new model for studies on human prostatic carcinoma.  Prog Clin Biol Res  1980  37:115-32  PubMed ID: 384082  

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User's Publication
16838  Matsushita M, Fujita K, Hatano K, Hayashi T, Kayama H, Motooka D, Hase H, Yamamoto A, Uemura T, Yamamichi G, Tomiyama E, Koh Y, Kato T, Kawashima A, Uemura M, Nojima S, Imamura R, Mubeen A, Netto GJ, Tsujikawa K, Nakamura S, Takeda K, Morii E, Nonomura N.  High-fat diet promotes prostate cancer growth through histamine signaling  Int J Cancer  2022  151(4):623-636  PubMed ID: 35403732   DOI: 10.1002/ijc.34028
20799  Fujimoto M, Takii R, Matsumoto M, Okada M, Nakayama KI, Nakato R, Fujiki K, Shirahige K, Nakai A.  HSF1 phosphorylation establishes an active chromatin state via the TRRAP-TIP60 complex and promotes tumorigenesis.  Nat Commun  2022  13(1):4355  PubMed ID: 35906200   DOI: 10.1038/s41467-022-32034-4
20927  Nakamura M, Fujiwara K, Doi N.  Cytoplasmic delivery of siRNA using human-derived membrane penetration-enhancing peptide.  J Nanobiotechnology  2022  20(1):458  PubMed ID: 36303212   DOI: 10.1186/s12951-022-01667-4
17315  Watabe T, Kaneda-Nakashima K, Shirakami Y, Kadonaga Y, Ooe K, Wang Y, Haba H, Toyoshima A, Cardinale J, Giesel FL, Tomiyama N, Fukase K.  Targeted α-therapy using astatine (211At)-labeled PSMA1, 5, and 6: a preclinical evaluation as a novel compound  Eur J Nucl Med Mol Imaging  2022  Nov 8  PubMed ID: 36344651   DOI: 10.1007/s00259-022-06016-z
13130  Sasaki K, Harada M, Yoshikawa T, Tagawa H, Harada Y, Yonemitsu Y, Ryujin T, Kishimura A, Mori T, Katayama Y.  Fc-Binding Antibody-Recruiting Molecules Targeting Prostate-Specific Membrane Antigen: Defucosylation of Antibody for Efficacy Improvement*.  Chembiochem  2021  22(3):496-500  PubMed ID: 32969164   DOI: 10.1002/cbic.202000577
13393  Miyasaka K, Takeda S, Nakamura S, Matsuda H, Shimoda H.  Estrogenic and antiandrogenic activities of methoxyflavans isolated from the fruit of Mauritia Flexuosa (Moriche Palm).  J Food Biochem  2021    PubMed ID: 33340139   DOI: 10.1111/jfbc.13583
20249  Makise M, Uchimura R, Higashi K, Mashiki Y, Shiraishi R, Shutoku Y, Kuniyasu A.  Overexpression of the nucleoporin Nup88 stimulates migration and invasion of HeLa cells.  Histochem Cell Biol  2021    PubMed ID: 34331103   DOI: 10.1007/s00418-021-02020-w
15709  Heishima K, Sugito N, Soga T, Nishikawa M, Ito Y, Honda R, Kuranaga Y, Sakai H, Ito R, Nakagawa T, Ueda H, Akao Y.  Petasin potently inhibits mitochondrial complex I-based metabolism that supports tumor growth and metastasis  J Clin Invest   2021  131(17):e139933  PubMed ID: 34623325   DOI: 10.1172/JCI139933
20406  Sawa C, Yofu S, Kiriyama K, Sutoh K, Saito T, Kishi S, Gunji M, Inoue Y, Sugi M, Shioda S, Honda K.  High concentration of extracellular nucleotides suppresses cell growth via delayed cell cycle progression in cancer and noncancer cell lines.  Heliyon  2021  7(11):e08318  PubMed ID: 34816032   DOI: 10.1016/j.heliyon.2021.e08318
16986  Nishimura K, Mori J, Sawada T, Nomura S, Kouzmenko A, Yamashita K, Kanemoto Y, Kurokawa T, Hayakawa A, Tokiwa S, Ochi M, Shimmura H, Kato S.  Profiling of Androgen-Dependent Enhancer RNAs Expression in Human Prostate Tumors: Search for Malignancy Transition Markers  Res Rep Urol  2021  13:705-713  PubMed ID: 34549035   DOI: 10.2147/RRU.S328661
17035  Ohya S, Kajikuri J, Endo K, Kito H, Matsui M.  K Ca 1.1 K + Channel Inhibition Overcomes Resistance to Antiandrogens and Doxorubicin in a Human Prostate Cancer LNCaP Spheroid Model  Int J Mol Sci  2021  22(24):13553  PubMed ID: 34948357   DOI: 10.3390/ijms222413553
12056  Ideo H, Kondo J, Nomura T, Nonomura N, Inoue M, Amano J.  Study of glycosylation of prostate-specific antigen secreted by cancer tissue-originated spheroids reveals new candidates for prostate cancer detection.  Sci Rep  2020    PubMed ID: 32066783   DOI: 10.1038/s41598-020-59622-y
13264  Hikita T, Miyata M, Watanabe R, Oneyama C.  In vivo imaging of long-term accumulation of cancer-derived exosomes using a BRET-based reporter.  Sci Rep  2020    PubMed ID: 33024173   DOI: 10.1038/s41598-020-73580-5
13317  Fujita K, Kamiya M, Yoshioka T, Ogasawara A, Hino R, Kojima R, Ueo H, Urano Y.  Rapid and Accurate Visualization of Breast Tumors with a Fluorescent Probe Targeting α-Mannosidase 2C1.  ACS Cent Sci  2020    PubMed ID: 33376783   DOI: 10.1021/acscentsci.0c01189
13591  Saiki I, Yara M, Yamanaka T, Uchino H, Inazu M.  Functional Expression of Choline Transporter-Like Protein 1 in LNCaP Prostate Cancer Cells: A Novel Molecular Target  Biomol Ther (Seoul)  2020  28(2):195-201  PubMed ID: 31693854   DOI: 10.4062/biomolther.2019.097
14832  Saito S, Kitamura-Muramatsu Y, Komine F, Polat M, Takeshima SN, Takei M, Aida Y.  Absence of bovine leukemia virus proviral DNA in Japanese human blood cell lines and human cancer cell lines  Arch Virol  2020  165(1):207-214  PubMed ID: 31776677   DOI: 10.1007/s00705-019-04474-9
11446  Wada A, Terashima T, Kageyama S, Yoshida T, Narita M, Kawauchi A, Kojima H.  Efficient Prostate Cancer Therapy with Tissue-Specific Homing Peptides Identified by Advanced Phage Display Technology.  Mol Ther Oncolytics  2019    PubMed ID: 30788426   DOI: 10.1016/j.omto.2019.01.001
11507  Murata K, Saga R, Monzen S, Tsuruga E, Hasegawa K, Hosokawa Y.  Understanding the mechanism underlying the acquisition of radioresistance in human prostate cancer cells.  Oncol Lett  2019    PubMed ID: 31186811   DOI: 10.3892/ol.2019.10219
11533  Saga R, Matsuya Y, Takahashi R, Hasegawa K, Date H, Hosokawa Y.  Analysis of the high-dose-range radioresistance of prostate cancer cells, including cancer stem cells, based on a stochastic model.  J. Radiat. Res.  2019    PubMed ID: 31034058   DOI: 10.1093/jrr/rrz011
11591  Kawatani M, Yamamoto K, Yamada D, Kamiya M, Miyakawa J, Miyama Y, Kojima R, Morikawa T, Kume H, Urano Y.  Fluorescence Detection of Prostate Cancer by an Activatable Fluorescence Probe for PSMA Carboxypeptidase Activity.  J. Am. Chem. Soc.  2019    PubMed ID: 31244179   DOI: 10.1021/jacs.9b04412
11800  Koizumi A, Narita S, Nakanishi H, Ishikawa M, Eguchi S, Kimura H, Takasuga S, Huang M, Inoue T, Sasaki J, Yoshioka T, Habuchi T, Sasaki T.  Increased fatty acyl saturation of phosphatidylinositol phosphates in prostate cancer progression.  Sci Rep  2019    PubMed ID: 31520002   DOI: 10.1038/s41598-019-49744-3
11807  Takagi H, Ii H, Kageyama S, Hanada E, Taniguchi K, Yoshiya T, Chano T, Kawauchi A, Nakata S.  Blockade of γ-Glutamylcyclotransferase Enhances Docetaxel Growth Inhibition of Prostate Cancer Cells.  Anticancer Res.  2019    PubMed ID: 31519583   DOI: 10.21873/anticanres.13666
6922  Soeda F, Watabe T, Naka S, Liu Y, Horitsugi G, Neels OC, Kopka K, Tatsumi M, Shimosegawa E, Giesel FL, Hatazawa J.  Impact of 18F-PSMA-1007 Uptake in Prostate Cancer Using Different Peptide Concentrations: Preclinical PET/CT Study on Mice.  J Nucl Med  2019  60(11):1594-1599  PubMed ID: 30902876   DOI: 10.2967/jnumed.118.223479
9810  Junko Shibato, Fumiko Takenoya, Takahiro Hirabayashi, Ai Kimura, Yusuke Iwasaki, Yoko Toyoda, Motohide Hori, Shigeru Tamogami, Randeep Rakwal, Seiji Shioda  Towards Identification of Bioactive Compounds in Cold Vacuum Extracted Double Cherry Blossom (Gosen-Sakura) Leaves  Plant Signal Behav  2019  14(10):e1644594  PubMed ID: 31342859   DOI: 10.1080/15592324.2019.1644594
12506  Jin C, Zhao W, Zhang Z, Liu W.  Silencing circular RNA circZNF609 restrains growth, migration and invasion by up-regulating microRNA-186-5p in prostate cancer  Artif Cells Nanomed Biotechnol  2019  47(1):3350-3358  PubMed ID: 31387394   DOI: 10.1080/21691401.2019.1648281
11152  Kamei H, Noguchi K, Matsuda H, Murata K.  Screening of Euphorbiaceae Plant Extracts for Anti-5α-reductase.  Biol. Pharm. Bull.  2018  41(8):1307-1310  PubMed ID: 30068884   DOI: 10.1248/bpb.b18-00283
11156  Ito S, Kayukawa N, Ueda T, Taniguchi H, Morioka Y, Hongo F, Ukimura O.  MRGBP promotes AR-mediated transactivation of KLK3 and TMPRSS2 via acetylation of histone H2A.Z in prostate cancer cells.  Biochim Biophys Acta Gene Regul Mech  2018    PubMed ID: 30076933   DOI: 10.1016/j.bbagrm.2018.07.014
11160  Languon S, Tuffour I, Quayson EE, Appiah-Opong R, Quaye O.  In Vitro Evaluation of Cytotoxic Activities of Marketed Herbal Products in Ghana.  J Evid Based Integr Med  2018    PubMed ID: 30088418   DOI: 10.1177/2515690X18790723
4279  Ito S, Ueno A, Ueda T, Nakagawa H, Taniguchi H, Kayukawa N, Fujihara-Iwata A, Hongo F, Okihara K, Ukimura O.  CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells.  Oncotarget  2018    PubMed ID: 29707137   DOI: 10.18632/oncotarget.24824
14599  Senga S, Kawaguchi K, Kobayashi N, Ando A, Fujii H.  A novel fatty acid-binding protein 5-estrogen-related receptor α signaling pathway promotes cell growth and energy metabolism in prostate cancer cells  Oncotarget  2018  9(60):31753-31770  PubMed ID: 30167092   DOI: 10.18632/oncotarget.25878
10057  Li X, Wang X, Zhang J, Hanagata N, Wang X, Weng Q, Ito A, Bando Y, Golberg D.  Hollow boron nitride nanospheres as boron reservoir for prostate cancer treatment.  Nat Commun  2017  8:13936  PubMed ID: 28059072   DOI: 10.1038/ncomms13936
10403  Qin H, Rasul A, Li X, Masood M, Yang G, Wang N, Wei W, He X, Watanabe N, Li J, Li X.  CD147-induced cell proliferation is associated with Smad4 signal inhibition.  Exp. Cell Res.  2017  358:279-289  PubMed ID: 28684116   DOI: 10.1016/j.yexcr.2017.07.003
10514  Kanayama M, Hayano T, Koebis M, Maeda T, Tabe Y, Horie S, Aiba A.  Hyperactive mTOR induces neuroendocrine differentiation in prostate cancer cell with concurrent up-regulation of IRF1.  Prostate  2017  77:1489-1498  PubMed ID: 28905415   DOI: 10.1002/pros.23425
11072  Hagiwara K, Tobisawa Y, Kaya T, Kaneko T, Hatakeyama S, Mori K, Hashimoto Y, Koie T, Suda Y, Ohyama C, Yoneyama T.  Wisteria floribunda Agglutinin and Its Reactive-Glycan-Carrying Prostate-Specific Antigen as a Novel Diagnostic and Prognostic Marker of Prostate Cancer.  Int J Mol Sci  2017    PubMed ID: 28134773   DOI: 10.3390/ijms18020261
4534  Cui C, Hanyu M, Hatori A, Zhang Y, Xie L, Ohya T, Fukada M, Suzuki H, Nagatsu K, Jiang C, Luo R, Shao G, Zhang M, Wang F.  Synthesis and evaluation of [64Cu]PSMA-617 targeted for prostate-specific membrane antigen in prostate cancer.  Am J Nucl Med Mol Imaging  2017  7(2):40-52  PubMed ID: 28533936  
14781  Fukami K, Asano E, Ueda M, Sekiguchi F, Yoshida S, Kawabata A.  High glucose induces N-linked glycosylation-mediated functional upregulation and overexpression of Ca v 3.2 T-type calcium channels in neuroendocrine-like differentiated human prostate cancer cells  J Pharmacol Sci  2017  133(1):57-6  PubMed ID: 28081948   DOI: 10.1016/j.jphs.2016.12.004
9650  Ogura T, Tanaka Y, Tamaki H, Harada M.  Docetaxel induces Bcl-2- and pro-apoptotic caspase-independent death of human prostate cancer DU145 cells.  Int. J. Oncol.  2016  48:2330-8  PubMed ID: 27082738   DOI: 10.3892/ijo.2016.3482
9862  Sakura Y, Tsuboi K, Uyama T, Zhang X, Taoka R, Sugimoto M, Kakehi Y, Ueda N.  A quantitative study on splice variants of N-acylethanolamine acid amidase in human prostate cancer cells and other cells.  Biochim. Biophys. Acta  2016  1861:1951-1958  PubMed ID: 27693242   DOI: 10.1016/j.bbalip.2016.09.018
9868  Ito H, Yamaguchi N, Onimaru M, Kimura S, Ohmori T, Ishikawa F, Sato J, Ito S, Inoue H.  Change in number and size of circulating tumor cells with high telomerase activity during treatment of patients with gastric cancer.  Oncol Lett  2016  12:4720-4726  PubMed ID: 28105179   DOI: 10.3892/ol.2016.5239
10024  Matsumura K, Nakata S, Taniguchi K, Ii H, Ashihara E, Kageyama S, Kawauchi A, Yoshiki T.  Depletion of γ-glutamylcyclotransferase inhibits breast cancer cell growth via cellular senescence induction mediated by CDK inhibitor upregulation.  BMC Cancer  2016  16:748  PubMed ID: 27658708   DOI: 10.1186/s12885-016-2779-y
10905    Roasted coffee induction of aldo-keto reductase 1C3 expression in LNCaP human prostate cancer cells is associated with Nrf2 activation  Oncology Letters  2016  12, 5321-5326    DOI: 10.3892/ol.2016.5334
12204  Minhui Xu, Shinichi Sakamoto, Jun Matsushima, Toru Kimura, Takeshi Ueda, Atsushi Mizokami, Yoshikatsu Kanai, Tomohiko Ichikawa  Up-Regulation of LAT1 during Antiandrogen Therapy Contributes to Progression in Prostate Cancer Cells  J Urol  2016  195(5):1588-1597  PubMed ID: 26682754   DOI: 10.1016/j.juro.2015.11.071
12621  Okada T, Uto K, Aoyagi T, Ebara M.  A biomimetic approach to hormone resistant prostate cancer cell isolation using inactivated Sendai virus (HVJ-E)  Biomater Sci  2016  4(1):96-103  PubMed ID: 26553555   DOI: 10.1039/c5bm00375j
12637  Kawaguchi K, Kinameri A, Suzuki S, Senga S, Ke Y, Fujii H.  The cancer-promoting gene fatty acid-binding protein 5 (FABP5) is epigenetically regulated during human prostate carcinogenesis  Biochem J  2016  473(4):449-61  PubMed ID: 26614767   DOI: 10.1042/BJ20150926
12660  Thangavel S, Yoshitomi T, Sakharkar MK, Nagasaki Y.  Redox nanoparticle increases the chemotherapeutic efficiency of pioglitazone and suppresses its toxic side effects  Biomaterials  2016  99:109-23  PubMed ID: 27235996   DOI: 10.1016/j.biomaterials.2016.05.001
12683  Matsushita S, Suzuki K, Ogino Y, Hino S, Sato T, Suyama M, Matsumoto T, Omori A, Inoue S, Yamada G.  Androgen Regulates Mafb Expression Through its 3'UTR During Mouse Urethral Masculinization  Endocrinology  2016  157(2):844-57.  PubMed ID: 26636186   DOI: 10.1210/en.2015-1586
14128  REGINA APPIAH-OPONG, ISAAC KWADWO ASANTE, DORCAS OSEI SAFO, ISAAC TUFFOUR, EBENEZER OFORI- ATTAH, TAKUHIRO UTO, ALEXANDER KWADWO NYARKO  Cytotoxic effects of Albizia zygia (DC) JF Macbr, a Ghanaian medicinal plant, against human T-lymphoblast-like leukemia, prostate and breast cancer cell lines  International Journal of Pharmacy and Pharmaceutical Sciences  2016  8   
15159  Honda S, Loher P, Shigematsu M, Palazzo JP, Suzuki R, Imoto I, Rigoutsos I, Kirino Y.  Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers  Proc Natl Acad Sci U S A  2015  112(29):E3816-25  PubMed ID: 26124144   DOI: 10.1073/pnas.1510077112
15450  Fukami K, Sekiguchi F, Yasukawa M, Asano E, Kasamatsu R, Ueda M, Yoshida S, Kawabata A.  Functional upregulation of the H2S/Cav3.2 channel pathway accelerates secretory function in neuroendocrine-differentiated human prostate cancer cells  Biochem Pharmacol  2015  97(3):300-9  PubMed ID: 26256074   DOI: 10.1016/j.bcp.2015.08.005
6398  Nagano K, Yamashita T, Inoue M, Higashisaka K, Yoshioka Y, Abe Y, Mukai Y, Kamada H, Tsutsumi Y, Tsunoda S  Eph receptor A10 has a potential as a target for a prostate cancer therapy.  Biochem Biophys Res Commun  2014  450(1):545-9  PubMed ID: 24924629   DOI: 10.1016/j.bbrc.2014.06.007
7747  Qin XY, Zaha H, Nagano R, Yoshinaga J, Yonemoto J, Sone H.  Xenoestrogens down-regulate aryl-hydrocarbon receptor nuclear translocator 2 mRNA expression in human breast cancer cells via an estrogen receptor alpha-dependent mechanism.  Toxicol. Lett.  2011  206:152-7  PubMed ID: 21771643   DOI: 10.1016/j.toxlet.2011.07.007
16162  Furuta RA, Miyazawa T, Sugiyama T, Kuratsune H, Ikeda Y, Sato E, Misawa N, Nakatomi Y, Sakuma R, Yasui K, Yamaguti K, Hirayama F.  No association of xenotropic murine leukemia virus-related virus with prostate cancer or chronic fatigue syndrome in Japan  Retrovirology  2011  8:20  PubMed ID: 21414229   DOI: 10.1186/1742-4690-8-20
8419  Fukushima K, Satoh T, Baba S, Yamashita K.  alpha1,2-Fucosylated and beta-N-acetylgalactosaminylated prostate-specific antigen as an efficient marker of prostatic cancer.  Glycobiology  2010  20:452-60  PubMed ID: 20008118   DOI: 10.1093/glycob/cwp197
10094  Fischer P, Kipp W, Bamuhiga J, Binta-Kahwa J, Kiefer A, Büttner DW.  Parasitological and clinical characterization of Simulium neavei-transmitted onchocerciasis in western Uganda.  Trop. Med. Parasitol.  1993  44:311-21  PubMed ID: 8134773  



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