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Cell No. : Cell Name
RCB2143 : DU145  update : 2022/12/06
CommentHuman cell line derived from brain metastasis of prostate carcinoma. TKG0604 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Stone, K.R.
Year of deposit 2005
Original cell TKG0604
Animal human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Age at sampling 69 years
Tissue prostate, brain meta
Disease name Prostate carcinoma brain metastasis
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0604)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0105
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin or 0.02% EDTA-PBS
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 42


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Reference
5113  Mickey DD, Stone KR, Wunderli H, Mickey GH, Paulson DF.  Characterization of a human prostate adenocarcinoma cell line (DU 145) as a monolayer culture and as a solid tumor in athymic mice.  Prog Clin Biol Res  1980  37:67-84  PubMed ID: 7384095  
5112  Stone KR, Mickey DD, Wunderli H, Mickey GH, Paulson DF.  Isolation of a human prostate carcinoma cell line (DU 145).  Int J Cancer  1978  21(3):274-81  PubMed ID: 631930   DOI: 10.1002/ijc.2910210305
5111  Mickey DD, Stone KR, Wunderli H, Mickey GH, Vollmer RT, Paulson DF.  Heterotransplantation of a human prostatic adenocarcinoma cell line in nude mice.  Cancer Res  1977  37(11):4049-58  PubMed ID: 908039  

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User's Publication
20504  Suzuki M, Iwaki K, Kikuchi M, Fujiwara K, Doi N.  Characterization of the membrane penetration-enhancing peptide S19 derived from human syncytin-1 for the intracellular delivery of TAT-fused proteins.  Biochem Biophys Res Commun  2022  586:63-67  PubMed ID: 34826702   DOI: 10.1016/j.bbrc.2021.11.065
20582  Qian Y, Liu X, Feng Y, Li X, Xuan Y.  Tenascin C regulates cancer cell glycolysis and tumor progression in prostate cancer.  Int J Urol  2022    PubMed ID: 35218089   DOI: 10.1111/iju.14830
20654  Akao Y, Terazawa R, Sugito N, Heishima K, Morikawa K, Ito Y, Narui R, Hamaguchi R, Nobukawa T.  Understanding of cell death induced by the constituents of Taxus yunnanensis wood.  Sci Rep  2022  12(1):6282  PubMed ID: 35428370   DOI: 10.1038/s41598-022-09655-2
16838  Matsushita M, Fujita K, Hatano K, Hayashi T, Kayama H, Motooka D, Hase H, Yamamoto A, Uemura T, Yamamichi G, Tomiyama E, Koh Y, Kato T, Kawashima A, Uemura M, Nojima S, Imamura R, Mubeen A, Netto GJ, Tsujikawa K, Nakamura S, Takeda K, Morii E, Nonomura N.  High-fat diet promotes prostate cancer growth through histamine signaling  Int J Cancer  2022  151(4):623-636  PubMed ID: 35403732   DOI: 10.1002/ijc.34028
13395  Imamura R, Kitagawa S, Kubo T, Irie A, Kariu T, Yoneda M, Kamba T, Imamura T.  Prostate cancer C5a receptor expression and augmentation of cancer cell proliferation, invasion, and PD-L1 expression by C5a.  Prostate  2021    PubMed ID: 33368414   DOI: 10.1002/pros.24090
20084  Matsuya Y, McMahon SJ, Butterworth KT, Naijo S, Nara I, Yachi Y, Saga R, Ishikawa M, Sato T, Date H, Prise KM.  Oxygen enhancement ratios of cancer cells after exposure to intensity modulated x-ray fields: DNA damage and cell survival.  Phys Med Biol  2021    PubMed ID: 33735839   DOI: 10.1088/1361-6560/abf011
20161  Maimaiti M, Sakamoto S, Sugiura M, Kanesaka M, Fujimoto A, Matsusaka K, Xu M, Ando K, Saito S, Wakai K, Imamura Y, Nakayama K, Kanai Y, Kaneda A, Ikehara Y, Ikeda JI, Anzai N, Ichikawa T.  The heavy chain of 4F2 antigen promote prostate cancer progression via SKP-2.  Sci Rep  2021  11(1):11478  PubMed ID: 34075107   DOI: 10.1038/s41598-021-90748-9
15709  Heishima K, Sugito N, Soga T, Nishikawa M, Ito Y, Honda R, Kuranaga Y, Sakai H, Ito R, Nakagawa T, Ueda H, Akao Y.  Petasin potently inhibits mitochondrial complex I-based metabolism that supports tumor growth and metastasis  J Clin Invest   2021  131(17):e139933  PubMed ID: 34623325   DOI: 10.1172/JCI139933
20436  Daikuzono H, Yamazaki M, Sato Y, Takahashi T, Yamagata K.  Development of a DELFIA method to detect oncofetal antigen ROR1-positive exosomes.  Biochem Biophys Res Commun  2021  578:170-176  PubMed ID: 34597914   DOI: 10.1016/j.bbrc.2021.08.054
15893  Matsushita M, Fujita K, Hayashi T, Kayama H, Motooka D, Hase H, Jingushi K, Yamamichi G, Yumiba S, Tomiyama E, Koh Y, Hayashi Y, Nakano K, Wang C, Ishizuya Y, Kato T, Hatano K, Kawashima A, Ujike T, Uemura M, Imamura R, Rodriguez Pena MDC, Gordetsky JB, Netto GJ, Tsujikawa K, Nakamura S, Takeda K, Nonomura N.  Gut Microbiota-Derived Short-Chain Fatty Acids Promote Prostate Cancer Growth via IGF1 Signaling  Cancer Res  2021  81(15):4014-4026  PubMed ID: 34039634   DOI: 10.1158/0008-5472.CAN-20-4090
11920  Takumi Chinen , Shohei Yamamoto, Yutaka Takeda , Koki Watanabe, Kanako Kuroki , Kaho Hashimoto , Daisuke Takao , Daiju Kitagawa  NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells  EMBO J  2020    PubMed ID: 31782546   DOI: 10.15252/embj.2019102378
13264  Hikita T, Miyata M, Watanabe R, Oneyama C.  In vivo imaging of long-term accumulation of cancer-derived exosomes using a BRET-based reporter.  Sci Rep  2020    PubMed ID: 33024173   DOI: 10.1038/s41598-020-73580-5
13317  Fujita K, Kamiya M, Yoshioka T, Ogasawara A, Hino R, Kojima R, Ueo H, Urano Y.  Rapid and Accurate Visualization of Breast Tumors with a Fluorescent Probe Targeting α-Mannosidase 2C1.  ACS Cent Sci  2020    PubMed ID: 33376783   DOI: 10.1021/acscentsci.0c01189
13591  Saiki I, Yara M, Yamanaka T, Uchino H, Inazu M.  Functional Expression of Choline Transporter-Like Protein 1 in LNCaP Prostate Cancer Cells: A Novel Molecular Target  Biomol Ther (Seoul)  2020  28(2):195-201  PubMed ID: 31693854   DOI: 10.4062/biomolther.2019.097
14832  Saito S, Kitamura-Muramatsu Y, Komine F, Polat M, Takeshima SN, Takei M, Aida Y.  Absence of bovine leukemia virus proviral DNA in Japanese human blood cell lines and human cancer cell lines  Arch Virol  2020  165(1):207-214  PubMed ID: 31776677   DOI: 10.1007/s00705-019-04474-9
11381  Win NN, Kyaw MM, Prema, Ngwe H, Ito T, Asakawa Y, Okamoto Y, Tanaka M, Abe I, Morita H.  Dinorcassane Diterpenoid from Boesenbergia rotunda Rhizomes Collected in Lower Myanmar.  Chem. Biodivers.  2019    PubMed ID: 30645035   DOI: 10.1002/cbdv.201800657
11507  Murata K, Saga R, Monzen S, Tsuruga E, Hasegawa K, Hosokawa Y.  Understanding the mechanism underlying the acquisition of radioresistance in human prostate cancer cells.  Oncol Lett  2019    PubMed ID: 31186811   DOI: 10.3892/ol.2019.10219
11533  Saga R, Matsuya Y, Takahashi R, Hasegawa K, Date H, Hosokawa Y.  Analysis of the high-dose-range radioresistance of prostate cancer cells, including cancer stem cells, based on a stochastic model.  J. Radiat. Res.  2019    PubMed ID: 31034058   DOI: 10.1093/jrr/rrz011
11618  Maehana S, Matsumoto Y, Kojima F, Kitasato H.  Interleukin-24 Transduction Modulates Human Prostate Cancer Malignancy Mediated by Regulation of Anchorage Dependence.  Anticancer Res.  2019    PubMed ID: 31262898   DOI: 10.21873/anticanres.13520
11800  Koizumi A, Narita S, Nakanishi H, Ishikawa M, Eguchi S, Kimura H, Takasuga S, Huang M, Inoue T, Sasaki J, Yoshioka T, Habuchi T, Sasaki T.  Increased fatty acyl saturation of phosphatidylinositol phosphates in prostate cancer progression.  Sci Rep  2019    PubMed ID: 31520002   DOI: 10.1038/s41598-019-49744-3
10845  Taniguchi K, Matsumura K, Ii H, Kageyama S, Ashihara E, Chano T, Kawauchi A, Yoshiki T, Nakata S.  Depletion of gamma-glutamylcyclotransferase in cancer cells induces autophagy followed by cellular senescence.  Am J Cancer Res  2018  8:650-661  PubMed ID: 29736310  
14599  Senga S, Kawaguchi K, Kobayashi N, Ando A, Fujii H.  A novel fatty acid-binding protein 5-estrogen-related receptor α signaling pathway promotes cell growth and energy metabolism in prostate cancer cells  Oncotarget  2018  9(60):31753-31770  PubMed ID: 30167092   DOI: 10.18632/oncotarget.25878
10246  Mabuchi M, Ueda M, Yoshida Y, Horiike K, Yamaoka K, Nakao S, Shimizu T, Ueda Y, Tsujikawa K, Tanaka A.  Systematic Trial for Evaluating Docetaxel in a Human Prostate Cancer Cell DU145 Xenograft Model.  Anticancer Res.  2017  37:1665-1676  PubMed ID: 28373427   DOI: 10.21873/anticanres.11496
9837  Ohnishi Y, Yasui H, Kakudo K, Nozaki M.  Lapatinib-resistant cancer cells possessing epithelial cancer stem cell properties develop sensitivity during sphere formation by activation of the ErbB/AKT/cyclin D2 pathway.  Oncol. Rep.  2016  36:3058-3064  PubMed ID: 27633099   DOI: 10.3892/or.2016.5073
9862  Sakura Y, Tsuboi K, Uyama T, Zhang X, Taoka R, Sugimoto M, Kakehi Y, Ueda N.  A quantitative study on splice variants of N-acylethanolamine acid amidase in human prostate cancer cells and other cells.  Biochim. Biophys. Acta  2016  1861:1951-1958  PubMed ID: 27693242   DOI: 10.1016/j.bbalip.2016.09.018
6034  Liu Q, Wu Y, Yoshizawa T, Yan X, Morohashi S, Seino H, Kato Y, Kijima H.  Basic helix-loop-helix transcription factor DEC2 functions as an anti-apoptotic factor during paclitaxel-induced apoptosis in human prostate cancer cells.  Int J Mol Med  2016  38(6):1727-1733  PubMed ID: 27840924   DOI: 10.3892/ijmm.2016.2798
12204  Minhui Xu, Shinichi Sakamoto, Jun Matsushima, Toru Kimura, Takeshi Ueda, Atsushi Mizokami, Yoshikatsu Kanai, Tomohiko Ichikawa  Up-Regulation of LAT1 during Antiandrogen Therapy Contributes to Progression in Prostate Cancer Cells  J Urol  2016  195(5):1588-1597  PubMed ID: 26682754   DOI: 10.1016/j.juro.2015.11.071
12637  Kawaguchi K, Kinameri A, Suzuki S, Senga S, Ke Y, Fujii H.  The cancer-promoting gene fatty acid-binding protein 5 (FABP5) is epigenetically regulated during human prostate carcinogenesis  Biochem J  2016  473(4):449-61  PubMed ID: 26614767   DOI: 10.1042/BJ20150926
14146  B Rupavani, V Peesapati, V G M Naiduc & S Ramakrishnad  Synthesis of some new physiologically active polyheterocycles derived from benzocyclohepten-5-ones  Indian Journal of Chemistry  2016  55B:88-93   
7189  Kim SH, Antfolk M, Kobayashi M, Kaneda S, Laurell T, Fujii T.  Highly efficient single cell arraying by integrating acoustophoretic cell pre-concentration and dielectrophoretic cell trapping.  Lab Chip  2015  15:4356-63  PubMed ID: 26439940   DOI: 10.1039/c5lc01065a
7215  Ohnuma T, Anzai E, Suzuki Y, Shimoda M, Saito S, Nishiyama T, Ogura K, Hiratsuka A.  Selective antagonization of activated Nrf2 and inhibition of cancer cell proliferation by procyanidins from Cinnamomi Cortex extract.  Arch. Biochem. Biophys.  2015  585:17-24  PubMed ID: 26365032   DOI: 10.1016/j.abb.2015.09.007
7507  Mabuchi M, Shimizu T, Ueda M, Sasakawa Y, Nakao S, Ueda Y, Kawamura A, Tsujikawa K, Tanaka A.  Improving the bioavailability and anticancer effect of the PCA-1/ALKBH3 inhibitor HUHS015 using sodium salt.  In Vivo  2015  29:39-43  PubMed ID: 25600528  
14955  Nishikawa Y, Okuzaki D, Fukushima K, Mukai S, Ohno S, Ozaki Y, Yabuta N, Nojima H.  Withaferin A Induces Cell Death Selectively in Androgen-Independent Prostate Cancer Cells but Not in Normal Fibroblast Cells  PLoS One  2015  10(7):e0134137  PubMed ID: 26230090   DOI: 10.1371/journal.pone.0134137
15159  Honda S, Loher P, Shigematsu M, Palazzo JP, Suzuki R, Imoto I, Rigoutsos I, Kirino Y.  Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers  Proc Natl Acad Sci U S A  2015  112(29):E3816-25  PubMed ID: 26124144   DOI: 10.1073/pnas.1510077112
15240  Kobayashi M, Kim SH, Nakamura H, Kaneda S, Fujii T.  Cancer Cell Analyses at the Single Cell-Level Using Electroactive Microwell Array Device  PLoS One  2015  10(11):e0139980  PubMed ID: 26558904   DOI: 10.1371/journal.pone.0139980
13624  Tamura M, Yanagawa F, Sugiura S, Takagi T, Sumaru K, Kanamori T.  Click-crosslinkable and photodegradable gelatin hydrogels for cytocompatible optical cell manipulation in natural environment  Sci Rep  2015  5:15060  PubMed ID: 26450015   DOI: 10.1038/srep15060
16006  Ramesh V, Ananda Rao B, Sharma P, Swarna B, Thummuri D, Srinivas K, Naidu VG, Jayathirtha Rao V.  Synthesis and biological evaluation of new rhodanine analogues bearing 2-chloroquinoline and benzo[h]quinoline scaffolds as anticancer agents  Eur J Med Chem  2014  83:569-80  PubMed ID: 24996143   DOI: 10.1016/j.ejmech.2014.06.013
14401  Yamaguchi Y, Deng D, Sato Y, Hou YT, Watanabe R, Sasaki K, Kawabe M, Hirano E, Morinaga T.  Silicate fiber-based 3D cell culture system for anticancer drug screening  Anticancer Res  2013  33(12):5301-9  PubMed ID: 24324063  
7689  Takagaki T, Bando T, Kitano M, Hashiya K, Kashiwazaki G, Sugiyama H.  Evaluation of PI polyamide conjugates with eight-base pair recognition and improvement of the aqueous solubility by PEGylation.  Bioorg. Med. Chem.  2011  19:5896-902  PubMed ID: 21903400   DOI: 10.1016/j.bmc.2011.08.009
7918  Higashimura Y, Nakajima Y, Yamaji R, Harada N, Shibasaki F, Nakano Y, Inui H.  Up-regulation of glyceraldehyde-3-phosphate dehydrogenase gene expression by HIF-1 activity depending on Sp1 in hypoxic breast cancer cells.  Arch. Biochem. Biophys.  2011  509:1-8  PubMed ID: 21338575   DOI: 10.1016/j.abb.2011.02.011
8143  Otake E, Sakuma S, Torii K, Maeda A, Ohi H, Yano S, Morita A.  Effect and mechanism of a new photodynamic therapy with glycoconjugated fullerene.  Photochem. Photobiol.  2010  86:1356-63  PubMed ID: 20796243   DOI: 10.1111/j.1751-1097.2010.00790.x
8419  Fukushima K, Satoh T, Baba S, Yamashita K.  alpha1,2-Fucosylated and beta-N-acetylgalactosaminylated prostate-specific antigen as an efficient marker of prostatic cancer.  Glycobiology  2010  20:452-60  PubMed ID: 20008118   DOI: 10.1093/glycob/cwp197



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