Back Back
Cell No. : Cell Name
RCB2143 : DU145  update : 2020/09/24
CommentHuman cell line derived from brain metastasis of prostate carcinoma. TKG0604 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Stone, K.R.
Year of deposit 2005
Original cell TKG0604
Animal human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Age at sampling 69 years
Tissue prostate, brain meta
Case history Prostate carcinoma brain metastasis
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0604)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin or 0.02% EDTA-PBS
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 3
User's Publication 19

To topTop
5113  Mickey DD, Stone KR, Wunderli H, Mickey GH, Paulson DF.  Characterization of a human prostate adenocarcinoma cell line (DU 145) as a monolayer culture and as a solid tumor in athymic mice.  Prog Clin Biol Res  1980  37:67-84  PubMed ID: 7384095
5112  Stone KR, Mickey DD, Wunderli H, Mickey GH, Paulson DF.  Isolation of a human prostate carcinoma cell line (DU 145).  Int J Cancer  1978  21(3):274-81  PubMed ID: 631930
5111  Mickey DD, Stone KR, Wunderli H, Mickey GH, Vollmer RT, Paulson DF.  Heterotransplantation of a human prostatic adenocarcinoma cell line in nude mice.  Cancer Res  1977  37(11):4049-58  PubMed ID: 908039

To topTop
User's Publication
11920  Takumi Chinen , Shohei Yamamoto, Yutaka Takeda , Koki Watanabe, Kanako Kuroki , Kaho Hashimoto , Daisuke Takao , Daiju Kitagawa  NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells  EMBO J  2020    PubMed ID: 31782546
11381  Win NN, Kyaw MM, Prema, Ngwe H, Ito T, Asakawa Y, Okamoto Y, Tanaka M, Abe I, Morita H.  Dinorcassane Diterpenoid from Boesenbergia rotunda Rhizomes Collected in Lower Myanmar.  Chem. Biodivers.  2019    PubMed ID: 30645035
11507  Murata K, Saga R, Monzen S, Tsuruga E, Hasegawa K, Hosokawa Y.  Understanding the mechanism underlying the acquisition of radioresistance in human prostate cancer cells.  Oncol Lett  2019    PubMed ID: 31186811
11533  Saga R, Matsuya Y, Takahashi R, Hasegawa K, Date H, Hosokawa Y.  Analysis of the high-dose-range radioresistance of prostate cancer cells, including cancer stem cells, based on a stochastic model.  J. Radiat. Res.  2019    PubMed ID: 31034058
11618  Maehana S, Matsumoto Y, Kojima F, Kitasato H.  Interleukin-24 Transduction Modulates Human Prostate Cancer Malignancy Mediated by Regulation of Anchorage Dependence.  Anticancer Res.  2019    PubMed ID: 31262898
11800  Koizumi A, Narita S, Nakanishi H, Ishikawa M, Eguchi S, Kimura H, Takasuga S, Huang M, Inoue T, Sasaki J, Yoshioka T, Habuchi T, Sasaki T.  Increased fatty acyl saturation of phosphatidylinositol phosphates in prostate cancer progression.  Sci Rep  2019    PubMed ID: 31520002
10845  Taniguchi K, Matsumura K, Ii H, Kageyama S, Ashihara E, Chano T, Kawauchi A, Yoshiki T, Nakata S.  Depletion of gamma-glutamylcyclotransferase in cancer cells induces autophagy followed by cellular senescence.  Am J Cancer Res  2018  8:650-661  PubMed ID: 29736310
10246  Mabuchi M, Ueda M, Yoshida Y, Horiike K, Yamaoka K, Nakao S, Shimizu T, Ueda Y, Tsujikawa K, Tanaka A.  Systematic Trial for Evaluating Docetaxel in a Human Prostate Cancer Cell DU145 Xenograft Model.  Anticancer Res.  2017  37:1665-1676  PubMed ID: 28373427
9837  Ohnishi Y, Yasui H, Kakudo K, Nozaki M.  Lapatinib-resistant cancer cells possessing epithelial cancer stem cell properties develop sensitivity during sphere formation by activation of the ErbB/AKT/cyclin D2 pathway.  Oncol. Rep.  2016  36:3058-3064  PubMed ID: 27633099
9862  Sakura Y, Tsuboi K, Uyama T, Zhang X, Taoka R, Sugimoto M, Kakehi Y, Ueda N.  A quantitative study on splice variants of N-acylethanolamine acid amidase in human prostate cancer cells and other cells.  Biochim. Biophys. Acta  2016  1861:1951-1958  PubMed ID: 27693242
6034  Liu Q, Wu Y, Yoshizawa T, Yan X, Morohashi S, Seino H, Kato Y, Kijima H.  Basic helix-loop-helix transcription factor DEC2 functions as an anti-apoptotic factor during paclitaxel-induced apoptosis in human prostate cancer cells.  Int J Mol Med  2016  38(6):1727-1733  PubMed ID: 27840924
12204  Minhui Xu, Shinichi Sakamoto, Jun Matsushima, Toru Kimura, Takeshi Ueda, Atsushi Mizokami, Yoshikatsu Kanai, Tomohiko Ichikawa  Up-Regulation of LAT1 during Antiandrogen Therapy Contributes to Progression in Prostate Cancer Cells  J Urol  2016  195(5):1588-1597  PubMed ID: 26682754
7189  Kim SH, Antfolk M, Kobayashi M, Kaneda S, Laurell T, Fujii T.  Highly efficient single cell arraying by integrating acoustophoretic cell pre-concentration and dielectrophoretic cell trapping.  Lab Chip  2015  15:4356-63  PubMed ID: 26439940
7215  Ohnuma T, Anzai E, Suzuki Y, Shimoda M, Saito S, Nishiyama T, Ogura K, Hiratsuka A.  Selective antagonization of activated Nrf2 and inhibition of cancer cell proliferation by procyanidins from Cinnamomi Cortex extract.  Arch. Biochem. Biophys.  2015  585:17-24  PubMed ID: 26365032
7507  Mabuchi M, Shimizu T, Ueda M, Sasakawa Y, Nakao S, Ueda Y, Kawamura A, Tsujikawa K, Tanaka A.  Improving the bioavailability and anticancer effect of the PCA-1/ALKBH3 inhibitor HUHS015 using sodium salt.  In Vivo  2015  29:39-43  PubMed ID: 25600528
7689  Takagaki T, Bando T, Kitano M, Hashiya K, Kashiwazaki G, Sugiyama H.  Evaluation of PI polyamide conjugates with eight-base pair recognition and improvement of the aqueous solubility by PEGylation.  Bioorg. Med. Chem.  2011  19:5896-902  PubMed ID: 21903400
7918  Higashimura Y, Nakajima Y, Yamaji R, Harada N, Shibasaki F, Nakano Y, Inui H.  Up-regulation of glyceraldehyde-3-phosphate dehydrogenase gene expression by HIF-1 activity depending on Sp1 in hypoxic breast cancer cells.  Arch. Biochem. Biophys.  2011  509:1-8  PubMed ID: 21338575
8143  Otake E, Sakuma S, Torii K, Maeda A, Ohi H, Yano S, Morita A.  Effect and mechanism of a new photodynamic therapy with glycoconjugated fullerene.  Photochem. Photobiol.  2010  86:1356-63  PubMed ID: 20796243
8419  Fukushima K, Satoh T, Baba S, Yamashita K.  alpha1,2-Fucosylated and beta-N-acetylgalactosaminylated prostate-specific antigen as an efficient marker of prostatic cancer.  Glycobiology  2010  20:452-60  PubMed ID: 20008118

Back Back Return Top Page