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細胞番号 : 細胞名
RCB2141 : PK-45P  update : 2024/11/12
細胞特性(Comment:英)Human cell line derived from pancreatic carcinoma. RCB1973 was derived from the same patient. TKG0493 (Deposited from Tohoku Univ.).
細胞特性(日)ヒト膵臓癌由来細胞株。RCB1973と同一患者由来。 TKG0493 (東北大学医用細胞資源センターからの寄託)。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Obinata, Masuo
樹立者 Kobori, M.
寄託日 2005
元の細胞 TKG0493
動物種 _human < Mammals
属名 Homo
種名 sapiens
採取組織 pancreas
病名 pancreas carcinoma
細胞分類 cancer
入手歴 Cell Resource Center for Biomedical Research, Tohoku University(TKG0493)
細胞寿命 infinite
細胞形態 other
Cellosaurus(Expasy) CVCL_6749
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 RPMI1640 + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : once/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ/アコレプラズマ (-)
アイソザイム検査 LD, NP
個体識別検査 OK
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 0件
Reference(日) 0件
利用者成果(英) 22件
利用者成果(日) 0件

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Reference(英)

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Reference(日)

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利用者成果(英)
19722  Masuda T, Fukuda A, Yamakawa G, Omatsu M, Namikawa M, Sono M, Fukunaga Y, Nagao M, Araki O, Yoshikawa T, Ogawa S, Masuo K, Goto N, Hiramatsu Y, Muta Y, Tsuda M, Maruno T, Nakanishi Y, Masui T, Hatano E, Matsuzaki T, Noda M, Seno H.  Pancreatic RECK inactivation promotes cancer formation, epithelial-mesenchymal transition, and metastasis  J Clin Invest  2023  133(18):e161847  PubMed ID: 37712427   DOI: 10.1172/JCI161847
19807  Shichi Y, Gomi F, Hasegawa Y, Nonaka K, Shinji S, Takahashi K, Ishiwata T.  Artificial intelligence-based analysis of time-lapse images of sphere formation and process of plate adhesion and spread of pancreatic cancer cells  Front Cell Dev Biol  2023  11:1290753  PubMed ID: 38046666   DOI: 10.3389/fcell.2023.1290753
18221  Shichi Y, Gomi F, Sasaki N, Nonaka K, Arai T, Ishiwata T.  Epithelial and Mesenchymal Features of Pancreatic Ductal Adenocarcinoma Cell Lines in Two- and Three-Dimensional Cultures  J Pers Med  2022  12(5):746  PubMed ID: 35629168   DOI: 10.3390/jpm12050746
20090  Minami F, Sasaki N, Shichi Y, Gomi F, Michishita M, Ohkusu-Tsukada K, Toyoda M, Takahashi K, Ishiwata T.  Morphofunctional analysis of human pancreatic cancer cell lines in 2- and 3-dimensional cultures.  Sci Rep  2021  11(1):6775  PubMed ID: 33762591   DOI: 10.1038/s41598-021-86028-1
13287  Sasaki N, Gomi F, Yoshimura H, Yamamoto M, Matsuda Y, Michishita M, Hatakeyama H, Kawano Y, Toyoda M, Korc M, Ishiwata T.  FGFR4 Inhibitor BLU9931 Attenuates Pancreatic Cancer Cell Proliferation and Invasion While Inducing Senescence: Evidence for Senolytic Therapy Potential in Pancreatic Cancer.  Cancers (Basel)  2020    PubMed ID: 33066597   DOI: 10.3390/cancers12102976
14280  Hobbs GA, Baker NM, Miermont AM, Thurman RD, Pierobon M, Tran TH, Anderson AO, Waters AM, Diehl JN, Papke B, Hodge RG, Klomp JE, Goodwin CM, DeLiberty JM, Wang J, Ng RWS, Gautam P, Bryant KL, Esposito D, Campbell SL, Petricoin EF 3rd, Simanshu DK, Aguirre AJ, Wolpin BM, Wennerberg K, Rudloff U, Cox AD, Der CJ.  Atypical KRAS G12R Mutant Is Impaired in PI3K Signaling and Macropinocytosis in Pancreatic Cancer  Cancer Discov  2020  10(1):104-123  PubMed ID: 31649109   DOI: 10.1158/2159-8290.CD-19-1006
5027  Nishizawa N1, Harada H1, Kumamoto Y1, Kaizu T, Katoh H, Tajima H, Ushiku H, Yokoi K, Igarashi K, Fujiyama Y, Okuwaki K, Iwai T, Watanabe M, Yamashita K1  Diagnostic potential of hypermethylation of the cysteine dioxygenase 1 gene (CDO1) promoter DNA in pancreatic cancer.  Cancer Sci  2019  110(9):2846-2855  PubMed ID: 31325200   DOI: 10.1111/cas.14134
11811  Mohammad GH, Vassileva V, Acedo P, Olde Damink SWM, Malago M, Dhar DK, Pereira SP.  Targeting Pyruvate Kinase M2 and Lactate Dehydrogenase A Is an Effective Combination Strategy for the Treatment of Pancreatic Cancer.  Cancers (Basel)  2019  11(9):1372  PubMed ID: 31527446   DOI: 10.3390/cancers11091372
11960  Sasaki N, Hirabayashi K, Michishita M, Takahashi K, Hasegawa F, Gomi F, Itakura Y, Nakamura N, Toyoda M, Ishiwata T.  Ganglioside GM2, highly expressed in the MIA PaCa-2 pancreatic ductal adenocarcinoma cell line, is correlated with growth, invasion, and advanced stage.  Sci Rep  2019    PubMed ID: 31852956   DOI: 10.1038/s41598-019-55867-4
4323  Chiba M, Kubota S, Sakai A, Monzen S.  Cell‑to‑cell communication via extracellular vesicles among human pancreatic cancer cells derived from the same patient.  Mol Med Rep  2018    PubMed ID: 30106154   DOI: 10.3892/mmr.2018.9376
10166  Kanno S, Nosho K, Ishigami K, Yamamoto I, Koide H, Kurihara H, Mitsuhashi K, Shitani M, Motoya M, Sasaki S, Tanuma T, Maguchi H, Hasegawa T, Kimura Y, Takemasa I, Shinomura Y, Nakase H.  MicroRNA-196b is an independent prognostic biomarker in patients with pancreatic cancer.  Carcinogenesis  2017  38:425-431  PubMed ID: 28186267   DOI: 10.1093/carcin/bgx013
10320  Kasai S, Sasaki T, Watanabe A, Nishiya M, Yasuhira S, Shibazaki M, Maesawa C.  Bcl-2/Bcl-xL inhibitor ABT-737 sensitizes pancreatic ductal adenocarcinoma to paclitaxel-induced cell death.  Oncol Lett  2017  14:903-908  PubMed ID: 28693250   DOI: 10.3892/ol.2017.6211
9938  Komura D, Isagawa T, Kishi K, Suzuki R, Sato R, Tanaka M, Katoh H, Yamamoto S, Tatsuno K, Fukayama M, Aburatani H, Ishikawa S.  CASTIN: a system for comprehensive analysis of cancer-stromal interactome.  BMC Genomics  2016  17:899  PubMed ID: 27829362   DOI: 10.1186/s12864-016-3207-z
12659  Mohammad GH, Olde Damink SW, Malago M, Dhar DK, Pereira SP.  Pyruvate Kinase M2 and Lactate Dehydrogenase A Are Overexpressed in Pancreatic Cancer and Correlate with Poor Outcome  PLoS One  2016  11(3):e0151635  PubMed ID: 26989901   DOI: 10.1371/journal.pone.0151635
7334  Ito H, Hasegawa K, Hasegawa Y, Nishimaki T, Hosomichi K, Kimura S, Ohba M, Yao H, Onimaru M, Inoue I, Inoue H.  Silver Nanoscale Hexagonal Column Chips for Detecting Cell-free DNA and Circulating Nucleosomes in Cancer Patients.  Sci Rep  2015  5:10455  PubMed ID: 25994878   DOI: 10.1038/srep10455
7348  Kobashigawa S, Morikawa K, Mori H, Kashino G.  Gemcitabine Induces Radiosensitization Through Inhibition of RAD51-dependent Repair for DNA Double-strand Breaks.  Anticancer Res.  2015  35:2731-7  PubMed ID: 25964552  
7362  Takeuchi S, Baghdadi M, Tsuchikawa T, Wada H, Nakamura T, Abe H, Nakanishi S, Usui Y, Higuchi K, Takahashi M, Inoko K, Sato S, Takano H, Shichinohe T, Seino K, Hirano S.  Chemotherapy-Derived Inflammatory Responses Accelerate the Formation of Immunosuppressive Myeloid Cells in the Tissue Microenvironment of Human Pancreatic Cancer.  Cancer Res.  2015  75:2629-40  PubMed ID: 25952647   DOI: 10.1158/0008-5472.CAN-14-2921
6384  Hidenori Kawashima Sakae Masaki Marie Kawamura  Induction of HLA‑A* 33‑restricted cytotoxic lymphocytes against renal cell carcinoma targeting galectin 9 and PINCH  Biomedical Reports  2014  2:809-812  PubMed ID: 25279150   DOI: 10.3892/br.2014.334
3714  Kato N, Yamamoto H, Adachi Y, Ohashi H, Taniguchi H, Suzuki H, Nakazawa M, Kaneto H, Sasaki S, Imai K, Shinomura Y.  Cancer detection by ubiquitin carboxyl-terminal esterase L1 methylation in pancreatobiliary fluids.  World J Gastroenterol  2013  19(11):1718-27  PubMed ID: 23555160   DOI: 10.3748/wjg.v19.i11.1718
5026  Waraya M, Yamashita K, Katoh H, Ooki A, Kawamata H, Nishimiya H, Nakamura K, Ema A, Watanabe M.  Cancer specific promoter CpG Islands hypermethylation of HOP homeobox (HOPX) gene and its potential tumor suppressive role in pancreatic carcinogenesis.  BMC Cancer  2012  12:397  PubMed ID: 22958219   DOI: 10.1186/1471-2407-12-397
7928  Shoji Y, Miyamoto M, Ishikawa K, Yoshioka T, Mishra R, Ichinokawa K, Matsumura Y, Itoh T, Shinohara T, Hirano S, Kondo S.  The CD40-CD154 interaction would correlate with proliferation and immune escape in pancreatic ductal adenocarcinoma.  J Surg Oncol  2011  103:230-8  PubMed ID: 21337550   DOI: 10.1002/jso.21812
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x

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