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Cell No. : Cell Name
RCB2138 : KLM-1  update : 2024/09/02
CommentHuman cell line derived from pancreatic cancer. RCB1972 was derived from the same patient. TKG0490 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Kobari, M.
Year of deposit 2005
Original cell TKG0490
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue pancreas
Disease name pancreatic carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0490)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_5146
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
11,12 10 11 12 15,19 9 X,Y 9,11 12
Images
deposit info
lot info
Reference information Reference 1
User's Publication 33


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Reference
5062  Kimura Y, Kobari M, Yusa T, Sunamura M, Kimura M, Shimamura H, Matsuno S.  Establishment of an experimental liver metastasis model by intraportal injection of a newly derived human pancreatic cancer cell line (KLM-1).  Int J Pancreatol  1996  20(1):43-50  PubMed ID: 8872523   DOI: 10.1007/BF02787375

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User's Publication
21666  Zhang Z, Aoki H, Umezawa K, Kranrod J, Miyazaki N, Oshima T, Hirao T, Miura Y, Seubert J, Ito K, Aoki S.  Potential role of lipophagy impairment for anticancer effects of glycolysis-suppressed pancreatic ductal adenocarcinoma cells.  Cell Death Discov  2024  10(1):166  PubMed ID: 38580661   DOI: 10.1038/s41420-024-01933-4
18285  Yamakawa K, Koyanagi-Aoi M, Machinaga A, Kakiuchi N, Hirano T, Kodama Y, Aoi T.  Blockage of retinoic acid signaling via RARγ suppressed the proliferation of pancreatic cancer cells by arresting the cell cycle progression of the G1-S phase  Cancer Cell Int  2023  23(1):94  PubMed ID: 37198667   DOI: 10.1186/s12935-023-02928-4
19924  Yoo HB, Moon JW, Kim HR, Lee HS, Miyabayashi K, Park CH, Ge S, Zhang A, Tae YK, Sub Y, Park HW, Gee HY, Notta F, Tuveson DA, Bang S, Kim MY, Roe JS.  A TEAD2-Driven Endothelial-Like Program Shapes Basal-Like Differentiation and Metastasis of Pancreatic Cancer  Gastroenterology  2023  165(1):133-148.e17  PubMed ID: 36907523   DOI: 10.1053/j.gastro.2023.02.049
21434  Rajbhandari N, Hamilton M, Quintero CM, Ferguson LP, Fox R, Schürch CM, Wang J, Nakamura M, Lytle NK, McDermott M, Diaz E, Pettit H, Kritzik M, Han H, Cridebring D, Wen KW, Tsai S, Goggins MG, Lowy AM, Wechsler-Reya RJ, Von Hoff DD, Newman AM, Reya T.  Single-cell mapping identifies MSI+ cells as a common origin for diverse subtypes of pancreatic cancer.  Cancer Cell  2023    PubMed ID: 37802055   DOI: 10.1016/j.ccell.2023.09.008
20570  Vaes RDW, van Dijk DPJ, Farshadi EA, Olde Damink SWM, Rensen SS, Langen RC.  Human pancreatic tumour organoid-derived factors enhance myogenic differentiation.  J Cachexia Sarcopenia Muscle  2022    PubMed ID: 35146962   DOI: 10.1002/jcsm.12917
20717  Yamakawa K, Koyanagi-Aoi M, Uehara K, Masuda A, Yanagimoto H, Toyama H, Fukumoto T, Kodama Y, Aoi T.  Increased expression of SPRR1A is associated with a poor prognosis in pancreatic ductal adenocarcinoma.  PLoS One  2022  17(5)  PubMed ID: 35617311   DOI: 10.1371/journal.pone.0266620
20747  Kabashima A, Matsuo Y, Ito S, Akiyama Y, Ishii T, Shimada S, Masamune A, Tanabe M, Tanaka S.  cGAS-STING signaling encourages immune cell overcoming of fibroblast barricades in pancreatic cancer.  Sci Rep  2022  12(1):10466  PubMed ID: 35773436   DOI: 10.1038/s41598-022-14297-5
20976  Liu Z, Hayashi H, Matsumura K, Ogata Y, Sato H, Shiraishi Y, Uemura N, Miyata T, Higashi T, Nakagawa S, Mima K, Imai K, Baba H.  Hyperglycaemia induces metabolic reprogramming into a glycolytic phenotype and promotes epithelial-mesenchymal transitions via YAP/TAZ-Hedgehog signalling axis in pancreatic cancer.  Br J Cancer  2022    PubMed ID: 36536047   DOI: 10.1038/s41416-022-02106-9
20108  Tasaki Y, Suzuki M, Katsushima K, Shinjo K, Iijima K, Murofushi Y, Naiki-Ito A, Hayashi K, Qiu C, Takahashi A, Tanaka Y, Kawaguchi T, Sugawara M, Kataoka T, Naito M, Miyata K, Kataoka K, Noda T, Gao W, Kataoka H, Takahashi S, Kimura K, Kondo Y.  Cancer-Specific Targeting of Taurine-Upregulated Gene 1 Enhances the Effects of Chemotherapy in Pancreatic Cancer.  Cancer Res  2021    PubMed ID: 33648930   DOI: 10.1158/0008-5472.CAN-20-3021
20289  Brennan G, Ryan S, Soulimane T, Tofail SAM, Silien C.  Dark Field and Coherent Anti-Stokes Raman (DF-CARS) Imaging of Cell Uptake of Core-Shell, Magnetic-Plasmonic Nanoparticles.  Nanomaterials (Basel)  2021  11(3)  PubMed ID: 33803430   DOI: 10.3390/nano11030685
20433  Bitorina AV, Oligschlaeger Y, Ding L, Yadati T, Westheim A, Houben T, Vaes RDW, Olde Damink SWM, Theys J, Shiri-Sverdlov R.  OxLDL as an Inducer of a Metabolic Shift in Cancer Cells.  J Cancer  2021  12(19):5817-5824  PubMed ID: 34475995   DOI: 10.7150/jca.56307
13240  Onodera T, Momose I, Adachi H, Yamazaki Y, Sawa R, Ohba SI, Kawada M.  Human pancreatic cancer cells under nutrient deprivation are vulnerable to redox system inhibition.  J Biol Chem  2020    PubMed ID: 32978257   DOI: 10.1074/jbc.RA120.013893
14803  Kushibiki T, Nakamura T, Tsuda M, Tsuchikawa T, Hontani K, Inoko K, Takahashi M, Asano T, Okamura K, Murakami S, Kurashima Y, Ebihara Y, Noji T, Nakanishi Y, Tanaka K, Maishi N, Sasaki K, Park WR, Shichinohe T, Hida K, Tanaka S, Hirano S.  Role of Dimerized C16orf74 in Aggressive Pancreatic Cancer: A Novel Therapeutic Target  Mol Cancer Ther  2020  19(1):187-198  PubMed ID: 31597713   DOI: 10.1158/1535-7163.MCT-19-0491
5030  Tsukamoto M, Imai K, Ishimoto T, Komohara Y, Yamashita YI, Nakagawa S, Umezaki N, Yamao T, Kitano Y, Miyata T, Arima K, Okabe H, Baba Y, Chikamoto A, Ishiko T, Hirota M, Baba H.  PD-L1 expression enhancement by infiltrating macrophage-derived tumor necrosis factor-α leads to poor pancreatic cancer prognosis.  Cancer Sci  2019  110(1):310-320  PubMed ID: 30426611   DOI: 10.1111/cas.13874
11811  Mohammad GH, Vassileva V, Acedo P, Olde Damink SWM, Malago M, Dhar DK, Pereira SP.  Targeting Pyruvate Kinase M2 and Lactate Dehydrogenase A Is an Effective Combination Strategy for the Treatment of Pancreatic Cancer.  Cancers (Basel)  2019  11(9):1372  PubMed ID: 31527446   DOI: 10.3390/cancers11091372
11862  Afify SM, Chen L, Yan T, Calle AS, Nair N, Murakami C, Zahra MH, Okada N, Iwasaki Y, Seno A, Seno M.  Method to Convert Stem Cells into Cancer Stem Cells.  Methods Protoc  2019    PubMed ID: 31426391   DOI: 10.3390/mps2030071
9183  Maria New, Tim Van Acker Jun-Ichi Sakamaki, Ming Jiang, Rebecca E Saunders, Jaclyn Long, Victoria M-Y Wang, Axel Behrens, Joana Cerveira, Padhmanand Sudhakar, Tamas Korcsmaros, Harold B J Jefferies, Kevin M Ryan, Michael Howell, Sharon A Tooze  MDH1 and MPP7 Regulate Autophagy in Pancreatic Ductal Adenocarcinoma  Cancer Res  2019  79 (8), 1884-1898  PubMed ID: 30765601   DOI: 10.1158/0008-5472.CAN-18-2553
10320  Kasai S, Sasaki T, Watanabe A, Nishiya M, Yasuhira S, Shibazaki M, Maesawa C.  Bcl-2/Bcl-xL inhibitor ABT-737 sensitizes pancreatic ductal adenocarcinoma to paclitaxel-induced cell death.  Oncol Lett  2017  14:903-908  PubMed ID: 28693250   DOI: 10.3892/ol.2017.6211
10466  Machihara K, Tanaka H, Hayashi Y, Murakami I, Namba T.  Questiomycin A stimulates sorafenib-induced cell death via suppression of glucose-regulated protein 78.  Biochem. Biophys. Res. Commun.  2017  492:33-40  PubMed ID: 28811106   DOI: 10.1016/j.bbrc.2017.08.042
14655  Ohishi T, Yoshida H, Katori M, Migita T, Muramatsu Y, Miyake M, Ishikawa Y, Saiura A, Iemura SI, Natsume T, Seimiya H.  Tankyrase-Binding Protein TNKS1BP1 Regulates Actin Cytoskeleton Rearrangement and Cancer Cell Invasion  Cancer Res  2017  77(9):2328-2338  PubMed ID: 28202517   DOI: 10.1158/0008-5472.CAN-16-1846
7044  Calle AS, Nair N, Oo AK, Prieto-Vila M, Koga M, Khayrani AC, Hussein M, Hurley L, Vaidyanath A, Seno A, Iwasaki Y, Calle M, Kasai T, Seno M.  A new PDAC mouse model originated from iPSCs-converted pancreatic cancer stem cells (CSCcm).  Am J Cancer Res  2016  6:2799-2815  PubMed ID: 28042501  
9938  Komura D, Isagawa T, Kishi K, Suzuki R, Sato R, Tanaka M, Katoh H, Yamamoto S, Tatsuno K, Fukayama M, Aburatani H, Ishikawa S.  CASTIN: a system for comprehensive analysis of cancer-stromal interactome.  BMC Genomics  2016  17:899  PubMed ID: 27829362   DOI: 10.1186/s12864-016-3207-z
12659  Mohammad GH, Olde Damink SW, Malago M, Dhar DK, Pereira SP.  Pyruvate Kinase M2 and Lactate Dehydrogenase A Are Overexpressed in Pancreatic Cancer and Correlate with Poor Outcome  PLoS One  2016  11(3):e0151635  PubMed ID: 26989901   DOI: 10.1371/journal.pone.0151635
7300  Namba T, Kodama R, Moritomo S, Hoshino T, Mizushima T.  Zidovudine, an anti-viral drug, resensitizes gemcitabine-resistant pancreatic cancer cells to gemcitabine by inhibition of the Akt-GSK3β-Snail pathway.  Cell Death Dis  2015  6:e1795  PubMed ID: 26111057   DOI: 10.1038/cddis.2015.172
7425  Hollevoet K, Mason-Osann E, Muller F, Pastan I.  Methylation-associated partial down-regulation of mesothelin causes resistance to anti-mesothelin immunotoxins in a pancreatic cancer cell line.  PLoS ONE  2015  10:e0122462  PubMed ID: 25803818   DOI: 10.1371/journal.pone.0122462
5025  Sakamoto K, Imai K, Higashi T, Taki K, Nakagawa S, Okabe H, Nitta H, Hayashi H, Chikamoto A, Ishiko T, Beppu T, Baba H.  Significance of P-cadherin overexpression and possible mechanism of its regulation in intrahepatic cholangiocarcinoma and pancreatic cancer.  Cancer Sci  2015  106(9):1153-62  PubMed ID: 26132727   DOI: 10.1111/cas.12732
15159  Honda S, Loher P, Shigematsu M, Palazzo JP, Suzuki R, Imoto I, Rigoutsos I, Kirino Y.  Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers  Proc Natl Acad Sci U S A  2015  112(29):E3816-25  PubMed ID: 26124144   DOI: 10.1073/pnas.1510077112
15259  Namba T, Kodama R.  Avarol induces apoptosis in pancreatic ductal adenocarcinoma cells by activating PERK-eIF2α-CHOP signaling  Mar Drugs  2015  13(4):2376-89  PubMed ID: 25894488   DOI: 10.3390/md13042376
14319  Hollevoet K, Antignani A, Fitzgerald DJ, Pastan I.  Combining the antimesothelin immunotoxin SS1P with the BH3-mimetic ABT-737 induces cell death in SS1P-resistant pancreatic cancer cells  J Immunother  2014  37(1):8-15  PubMed ID: 24316551   DOI: 10.1097/CJI.0000000000000010
14322  Mattoo AR, Pastan I, Fitzgerald D.  Combination treatments with the PKC inhibitor, enzastaurin, enhance the cytotoxicity of the anti-mesothelin immunotoxin, SS1P  PLoS One  2013  8(10):e75576  PubMed ID: 24130723   DOI: 10.1371/journal.pone.0075576
15922  Fujitani N, Furukawa J, Araki K, Fujioka T, Takegawa Y, Piao J, Nishioka T, Tamura T, Nikaido T, Ito M, Nakamura Y, Shinohara Y.  Total cellular glycomics allows characterizing cells and streamlining the discovery process for cellular biomarkers  Proc Natl Acad Sci U S A   2013  110(6):2105-10  PubMed ID: 23345451   DOI: 10.1073/pnas.1214233110
19021  Awale S, Ueda JY, Athikomkulchai S, Abdelhamed S, Yokoyama S, Saiki I, Miyatake R.  Antiausterity agents from Uvaria dac and their preferential cytotoxic activity against human pancreatic cancer cell lines in a nutrient-deprived condition  J Nat Prod  2012  75(6):1177-83  PubMed ID: 22676269   DOI: 10.1021/np300295h
7928  Shoji Y, Miyamoto M, Ishikawa K, Yoshioka T, Mishra R, Ichinokawa K, Matsumura Y, Itoh T, Shinohara T, Hirano S, Kondo S.  The CD40-CD154 interaction would correlate with proliferation and immune escape in pancreatic ductal adenocarcinoma.  J Surg Oncol  2011  103:230-8  PubMed ID: 21337550   DOI: 10.1002/jso.21812



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