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Cell No. : Cell Name
RCB2138 : KLM-1  update : 2021/09/15
CommentHuman cell line derived from pancreatic cancer. RCB1972 was derived from the same patient. TKG0490 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Obinata, Masuo
Originator Kobari, M.
Year of deposit 2005
Original cell TKG0490
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue pancreas
Disease name pancreatic carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0490)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_5146
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
11,12 10 11 12 15,19 9 X,Y 9,11 12
Images
deposit info
lot info
Reference information Reference 1
User's Publication 22


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Reference
5062  Kimura Y, Kobari M, Yusa T, Sunamura M, Kimura M, Shimamura H, Matsuno S.  Establishment of an experimental liver metastasis model by intraportal injection of a newly derived human pancreatic cancer cell line (KLM-1).  Int J Pancreatol  1996  20(1):43-50  PubMed ID: 8872523

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User's Publication
20108  Tasaki Y, Suzuki M, Katsushima K, Shinjo K, Iijima K, Murofushi Y, Naiki-Ito A, Hayashi K, Qiu C, Takahashi A, Tanaka Y, Kawaguchi T, Sugawara M, Kataoka T, Naito M, Miyata K, Kataoka K, Noda T, Gao W, Kataoka H, Takahashi S, Kimura K, Kondo Y.  Cancer-Specific Targeting of Taurine-Upregulated Gene 1 Enhances the Effects of Chemotherapy in Pancreatic Cancer.  Cancer Res  2021    PubMed ID: 33648930
20289  Brennan G, Ryan S, Soulimane T, Tofail SAM, Silien C.  Dark Field and Coherent Anti-Stokes Raman (DF-CARS) Imaging of Cell Uptake of Core-Shell, Magnetic-Plasmonic Nanoparticles.  Nanomaterials (Basel)  2021  11(3)  PubMed ID: 33803430
13240  Onodera T, Momose I, Adachi H, Yamazaki Y, Sawa R, Ohba SI, Kawada M.  Human pancreatic cancer cells under nutrient deprivation are vulnerable to redox system inhibition.  J Biol Chem  2020    PubMed ID: 32978257
14803  Kushibiki T, Nakamura T, Tsuda M, Tsuchikawa T, Hontani K, Inoko K, Takahashi M, Asano T, Okamura K, Murakami S, Kurashima Y, Ebihara Y, Noji T, Nakanishi Y, Tanaka K, Maishi N, Sasaki K, Park WR, Shichinohe T, Hida K, Tanaka S, Hirano S.  Role of Dimerized C16orf74 in Aggressive Pancreatic Cancer: A Novel Therapeutic Target  Mol Cancer Ther  2020  19(1):187-198  PubMed ID: 31597713
5030  Tsukamoto M, Imai K, Ishimoto T, Komohara Y, Yamashita YI, Nakagawa S, Umezaki N, Yamao T, Kitano Y, Miyata T, Arima K, Okabe H, Baba Y, Chikamoto A, Ishiko T, Hirota M, Baba H.  PD-L1 expression enhancement by infiltrating macrophage-derived tumor necrosis factor-α leads to poor pancreatic cancer prognosis.  Cancer Sci  2019  110(1):310-320  PubMed ID: 30426611
11811  Mohammad GH, Vassileva V, Acedo P, Olde Damink SWM, Malago M, Dhar DK, Pereira SP.  Targeting Pyruvate Kinase M2 and Lactate Dehydrogenase A Is an Effective Combination Strategy for the Treatment of Pancreatic Cancer.  Cancers (Basel)  2019  11(9):1372  PubMed ID: 31527446
11862  Afify SM, Chen L, Yan T, Calle AS, Nair N, Murakami C, Zahra MH, Okada N, Iwasaki Y, Seno A, Seno M.  Method to Convert Stem Cells into Cancer Stem Cells.  Methods Protoc  2019    PubMed ID: 31426391
9183  Maria New, Tim Van Acker Jun-Ichi Sakamaki, Ming Jiang, Rebecca E Saunders, Jaclyn Long, Victoria M-Y Wang, Axel Behrens, Joana Cerveira, Padhmanand Sudhakar, Tamas Korcsmaros, Harold B J Jefferies, Kevin M Ryan, Michael Howell, Sharon A Tooze  MDH1 and MPP7 Regulate Autophagy in Pancreatic Ductal Adenocarcinoma  Cancer Res  2019  79 (8), 1884-1898  PubMed ID: 30765601
10320  Kasai S, Sasaki T, Watanabe A, Nishiya M, Yasuhira S, Shibazaki M, Maesawa C.  Bcl-2/Bcl-xL inhibitor ABT-737 sensitizes pancreatic ductal adenocarcinoma to paclitaxel-induced cell death.  Oncol Lett  2017  14:903-908  PubMed ID: 28693250
10466  Machihara K, Tanaka H, Hayashi Y, Murakami I, Namba T.  Questiomycin A stimulates sorafenib-induced cell death via suppression of glucose-regulated protein 78.  Biochem. Biophys. Res. Commun.  2017  492:33-40  PubMed ID: 28811106
14655  Ohishi T, Yoshida H, Katori M, Migita T, Muramatsu Y, Miyake M, Ishikawa Y, Saiura A, Iemura SI, Natsume T, Seimiya H.  Tankyrase-Binding Protein TNKS1BP1 Regulates Actin Cytoskeleton Rearrangement and Cancer Cell Invasion  Cancer Res  2017  77(9):2328-2338  PubMed ID: 28202517
7044  Calle AS, Nair N, Oo AK, Prieto-Vila M, Koga M, Khayrani AC, Hussein M, Hurley L, Vaidyanath A, Seno A, Iwasaki Y, Calle M, Kasai T, Seno M.  A new PDAC mouse model originated from iPSCs-converted pancreatic cancer stem cells (CSCcm).  Am J Cancer Res  2016  6:2799-2815  PubMed ID: 28042501
9938  Komura D, Isagawa T, Kishi K, Suzuki R, Sato R, Tanaka M, Katoh H, Yamamoto S, Tatsuno K, Fukayama M, Aburatani H, Ishikawa S.  CASTIN: a system for comprehensive analysis of cancer-stromal interactome.  BMC Genomics  2016  17:899  PubMed ID: 27829362
12659  Mohammad GH, Olde Damink SW, Malago M, Dhar DK, Pereira SP.  Pyruvate Kinase M2 and Lactate Dehydrogenase A Are Overexpressed in Pancreatic Cancer and Correlate with Poor Outcome  PLoS One  2016  11(3):e0151635  PubMed ID: 26989901
7300  Namba T, Kodama R, Moritomo S, Hoshino T, Mizushima T.  Zidovudine, an anti-viral drug, resensitizes gemcitabine-resistant pancreatic cancer cells to gemcitabine by inhibition of the Akt-GSK3β-Snail pathway.  Cell Death Dis  2015  6:e1795  PubMed ID: 26111057
7425  Hollevoet K, Mason-Osann E, Muller F, Pastan I.  Methylation-associated partial down-regulation of mesothelin causes resistance to anti-mesothelin immunotoxins in a pancreatic cancer cell line.  PLoS ONE  2015  10:e0122462  PubMed ID: 25803818
5025  Sakamoto K, Imai K, Higashi T, Taki K, Nakagawa S, Okabe H, Nitta H, Hayashi H, Chikamoto A, Ishiko T, Beppu T, Baba H.  Significance of P-cadherin overexpression and possible mechanism of its regulation in intrahepatic cholangiocarcinoma and pancreatic cancer.  Cancer Sci  2015  106(9):1153-62  PubMed ID: 26132727
15159  Honda S, Loher P, Shigematsu M, Palazzo JP, Suzuki R, Imoto I, Rigoutsos I, Kirino Y.  Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers  Proc Natl Acad Sci U S A  2015  112(29):E3816-25  PubMed ID: 26124144
15259  Namba T, Kodama R.  Avarol induces apoptosis in pancreatic ductal adenocarcinoma cells by activating PERK-eIF2α-CHOP signaling  Mar Drugs  2015  13(4):2376-89  PubMed ID: 25894488
14319  Hollevoet K, Antignani A, Fitzgerald DJ, Pastan I.  Combining the antimesothelin immunotoxin SS1P with the BH3-mimetic ABT-737 induces cell death in SS1P-resistant pancreatic cancer cells  J Immunother  2014  37(1):8-15  PubMed ID: 24316551
14322  Mattoo AR, Pastan I, Fitzgerald D.  Combination treatments with the PKC inhibitor, enzastaurin, enhance the cytotoxicity of the anti-mesothelin immunotoxin, SS1P  PLoS One  2013  8(10):e75576  PubMed ID: 24130723
7928  Shoji Y, Miyamoto M, Ishikawa K, Yoshioka T, Mishra R, Ichinokawa K, Matsumura Y, Itoh T, Shinohara T, Hirano S, Kondo S.  The CD40-CD154 interaction would correlate with proliferation and immune escape in pancreatic ductal adenocarcinoma.  J Surg Oncol  2011  103:230-8  PubMed ID: 21337550



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