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Cell No. : Cell Name
RCB2126 : BmN4-IR  update : 2023/10/16
CommentSilkworm (Bombyx mori) cell line derived from ovary. Useful for the identification and analysis of homologous recombination-related genes in the silkworm. Application consideration
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Kyushu university
Laboratory of Silkworm Science
Hakozaki 6-10-1, Higashi-ku, Fukuoka, 812-8581 Japan
Dr.KUSAKABE Takahiro
Fax. +81-92-642-2842
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kusakabe, Takahiro
Originator Mon, Hiroaki
Year of deposit 2005
Original cell BmN4
Animal _silkworm < Insect
Genus Bombyx
Species mori
Tissue ovary
Classification other
Recombinant recombinant
Exogene pSK8-Luc5'3'IR
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_Z090
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Culture medium IPL-41 + 10% FBS
Antibiotics Free
Passage method dilution, pipetting
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
deposit info
lot info
Reference information Reference 4
User's Publication 0

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5479  Tatsuke T, Sakashita K, Masaki Y, Lee JM, Kawaguchi Y, Kusakabe T.  The telomere-specific non-LTR retrotransposons SART1 and TRAS1 are suppressed by Piwi subfamily proteins in the silkworm, Bombyx mori.  Cell Mol Biol Lett  2010  15(1):118-33  PubMed ID: 19943120   DOI: 10.2478/s11658-009-0038-9
3485  Nakayama G, Kawaguchi Y, Koga K, Kusakabe T.  Site-specific gene integration in cultured silkworm cells mediated by phiC31 integrase.  Mol Genet Genomics  2006  275(1):1-8  PubMed ID: 16341709   DOI: 10.1007/s00438-005-0026-3
3486  Ohsaki A, Iiyama K, Miyagawa Y, Kawaguchi Y, Koga K, Kusakabe T.  Nonhomologous end-joining in a cell-free extract from the cultured silkworm cell line BmN4.  Mol Biol Rep  2005  32(1):25-34  PubMed ID: 15865207   DOI: 10.1007/s11033-004-2474-y
3484  Mon H, Kusakabe T, Bando H, Kojima K, Kawaguchi Y, Koga K.  Analysis of extrachromosomal homologous recombination in cultured silkworm cells.  Biochem Biophys Res Commun  2003  312(3):684-90  PubMed ID: 14680819   DOI: 10.1016/j.bbrc.2003.10.169

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