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Cell No. : Cell Name
RCB2120 : TSt-4/N-DLL1  update : 2021/05/20
CommentOriginal murine mesenchymal cell line was derived from fetal thymus tissue. By retrovirally introducing DLL1 gene, this cell line can support the generation of T cells (up to DP stage) from muirne hematopietic stem/progenitors. Human NGF receptor is also introduced, and is expressed as a marker on cell surface.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kawamoto, Hiroshi
Originator Kawamoto, Hiroshi
Year of deposit 2005
Animal mouse < Mammals
Age at sampling fetus
Tissue thymus
Classification transformed
Recombinant recombinant
Exogene MSCV-IRES-NGFR(human),murine DLL1 (delta-like 1)
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_B977
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 5% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
3312  Miyazaki, Masaki, Kawamoto, Hiroshi, Kato, Yuko, Itoi, Manami, Miyazaki, Kazuko, Masuda, Kyoko, Tashiro, Satoshi, Ishihara, Hiroto, Igarashi, Kazuhiko, Amagai, Takashi, Kanno, Rieko, Kanno, Masamoto  Polycomb group gene mel-18 regulates early T progenitor expansion by maintaining the expression of Hes-1, a target of the Notch pathway.  J Immunol  2005  174:2507-16  PubMed ID: 15728456

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User's Publication
8207  Kaori Yoshino, Kaoru Saijo, Chikako Noro and Yukio Nakamura  Development of a simple method to determine the mouse strain from which cultured cell lines originated  Interdisciplinary Bio Central   2010  2:0014 



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