Cell No. : Cell Name RCB2120 : TSt-4/N-DLL1  update : 2024/11/11
Comment			Original murine mesenchymal cell line was derived from fetal thymus tissue. By retrovirally introducing DLL1 gene, this cell line can support the generation of T cells (up to DP stage) from muirne hematopietic stem/progenitors. Human NGF receptor is also introduced, and is expressed as a marker on cell surface.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Kawamoto, Hiroshi
		Originator	Kawamoto, Hiroshi
		Year of deposit	2005
		Animal	_mouse < Mammals
		Age at sampling	fetus
		Tissue	thymus
		Classification	transformed
		Recombinant	recombinant
		Exogene	MSCV-IRES-NGFR(human),murine DLL1 (delta-like 1)
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_B977
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		RPMI1640 + 5% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma	(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	1

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Reference

3312  Miyazaki, Masaki, Kawamoto, Hiroshi, Kato, Yuko, Itoi, Manami, Miyazaki, Kazuko, Masuda, Kyoko, Tashiro, Satoshi, Ishihara, Hiroto, Igarashi, Kazuhiko, Amagai, Takashi, Kanno, Rieko, Kanno, Masamoto  Polycomb group gene mel-18 regulates early T progenitor expansion by maintaining the expression of Hes-1, a target of the Notch pathway.  J Immunol  2005  174:2507-16  PubMed ID: 15728456   DOI: 10.4049/jimmunol.174.5.2507

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User's Publication

8207  Kaori Yoshino, Kaoru Saijo, Chikako Noro and Yukio Nakamura  Development of a simple method to determine the mouse strain from which cultured cell lines originated  Interdisciplinary Bio Central   2010  2:0014    DOI: 10.4051/ibc.2010.2.4.0014