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Cell No. : Cell Name
RCB2116 : TSt-4  update : 2023/10/16
CommentMurine mesenchymal cell line derived from fetal thymus tissue. Can support the generation of myeloid cells and B cells from muirne hematopietic stem/progenitors.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kawamoto, Hiroshi
Originator Watanabe,Y. & Katsura, Y.
Year of deposit 2005
Animal _mouse < Mammals
Age at sampling fetus
Tissue thymus
Classification normal
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_B973
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 5% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 3

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3311  Ohmura K, Kawamoto H, Fujimoto S, Ozaki S, Nakao K, Katsura Y  Emergence of T, B, and myeloid lineage-committed as well as multipotent hemopoietic progenitors in the aorta-gonad-mesonephros region of day 10 fetuses of the mouse.  J Immunol  1999  163:4788-95  PubMed ID: 10528178  
3310  Watanabe Y, Mazda O, Aiba Y, Iwai K, Gyotoku J, Ideyama S, Miyazaki J, Katsura Y  A murine thymic stromal cell line which may support the differentiation of CD4-8- thymocytes into CD4+8- alpha beta T cell receptor positive T cells.  Cell Immunol  1992  142:385-97  PubMed ID: 1535830   DOI: 10.1016/0008-8749(92)90299-5

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User's Publication
14317  Hoellenriegel J, Zboralski D, Maasch C, Rosin NY, Wierda WG, Keating MJ, Kruschinski A, Burger JA.  The Spiegelmer NOX-A12, a novel CXCL12 inhibitor, interferes with chronic lymphocytic leukemia cell motility and causes chemosensitization  Blood  2014  123(7):1032-9  PubMed ID: 24277076   DOI: 10.1182/blood-2013-03-493924
7732  Hoellenriegel J, Meadows SA, Sivina M, Wierda WG, Kantarjian H, Keating MJ, Giese N, O'Brien S, Yu A, Miller LL, Lannutti BJ, Burger JA.  The phosphoinositide 3'-kinase delta inhibitor, CAL-101, inhibits B-cell receptor signaling and chemokine networks in chronic lymphocytic leukemia.  Blood  2011  118:3603-12  PubMed ID: 21803855   DOI: 10.1182/blood-2011-05-352492
8207  Kaori Yoshino, Kaoru Saijo, Chikako Noro and Yukio Nakamura  Development of a simple method to determine the mouse strain from which cultured cell lines originated  Interdisciplinary Bio Central   2010  2:0014    DOI: 10.4051/ibc.2010.2.4.0014

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