Cell No. : Cell Name
RCB2116 : TSt-4
update : 2023/10/16
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| Comment | Murine mesenchymal cell line derived from fetal thymus tissue. Can support the generation of myeloid cells and B cells from muirne hematopietic stem/progenitors. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Kawamoto, Hiroshi
|
| Originator |
Watanabe,Y. & Katsura, Y.
|
| Year of deposit |
2005
|
| Animal |
_mouse
< Mammals
|
| Age at sampling |
fetus
|
| Tissue |
thymus
|
| Classification |
normal
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_B973
|
|
| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
|
Adherent cells
|
| Culture medium |
|
RPMI1640 + 5% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
3
|
| Reference |
|
3311
Ohmura K, Kawamoto H, Fujimoto S, Ozaki S, Nakao K, Katsura Y
Emergence of T, B, and myeloid lineage-committed as well as multipotent hemopoietic progenitors in the aorta-gonad-mesonephros region of day 10 fetuses of the mouse.
J Immunol
1999
163:4788-95
PubMed ID: 10528178
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3310
Watanabe Y, Mazda O, Aiba Y, Iwai K, Gyotoku J, Ideyama S, Miyazaki J, Katsura Y
A murine thymic stromal cell line which may support the differentiation of CD4-8- thymocytes into CD4+8- alpha beta T cell receptor positive T cells.
Cell Immunol
1992
142:385-97
PubMed ID: 1535830
DOI: 10.1016/0008-8749(92)90299-5
|
| User's Publication |
|
14317
Hoellenriegel J, Zboralski D, Maasch C, Rosin NY, Wierda WG, Keating MJ, Kruschinski A, Burger JA.
The Spiegelmer NOX-A12, a novel CXCL12 inhibitor, interferes with chronic lymphocytic leukemia cell motility and causes chemosensitization
Blood
2014
123(7):1032-9
PubMed ID: 24277076
DOI: 10.1182/blood-2013-03-493924
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7732
Hoellenriegel J, Meadows SA, Sivina M, Wierda WG, Kantarjian H, Keating MJ, Giese N, O'Brien S, Yu A, Miller LL, Lannutti BJ, Burger JA.
The phosphoinositide 3'-kinase delta inhibitor, CAL-101, inhibits B-cell receptor signaling and chemokine networks in chronic lymphocytic leukemia.
Blood
2011
118:3603-12
PubMed ID: 21803855
DOI: 10.1182/blood-2011-05-352492
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8207
Kaori Yoshino, Kaoru Saijo, Chikako Noro and Yukio Nakamura
Development of a simple method to determine the mouse strain from which cultured cell lines originated
Interdisciplinary Bio Central
2010
2:0014
DOI: 10.4051/ibc.2010.2.4.0014
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